Proteintech

MMP12 Polyclonal Antibody

MMP12 Antibody in Immunocytochemistry (ICC/IF)

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MMP12 Antibody (22989-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed A549 cells using 22989-1-AP (MMP12 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MMP12 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

22989-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 5 publications 5 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Hamster, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MMP12 Fusion Protein Ag19068 (231-402 aa encoded by BC112301)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.52 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: DPKAVMFPT YKYVDINTFR LSADDIRGIQ SLYGDPKENQ RLPNPDNSEP ALCDPNLSFD AVTTVGNKIF FFKDRFFWLK VSERPKTSVN LISSLWPTLP SGIEAAYEIE ARNQVFLFKD DKYWLISNLR PEPNYPKSIH SFGFPNFVKK IDAAVFNPRF YRTYFFVDNQ YWR (231-402 aa encoded by BC112301)

Target Information

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22. 3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Characterization of a novel rabbit model of Peyronie's disease.

International journal of impotence research

Gundogdu G,Nguyen T,Namasivayam A,Starek S,Gelman J,Mauney JR

Wed May 01 00:00:00 UTC 2024

MicroRNA Let-7 Induces M2 Macrophage Polarization in COPD Emphysema Through the IL-6/STAT3 Pathway.

International journal of chronic obstructive pulmonary disease

Liu T,Zhang Z,Shen W,Wu Y,Bian T

Thu May 11 00:00:00 UTC 2023

Metformin Directly Binds to MMP-9 to Improve Plaque Stability.

Journal of cardiovascular development and disease

Chen X,Wang S,Xu W,Zhao M,Zhang Y,Xiao H

Mon Jan 30 00:00:00 UTC 2023

NCOA4-Mediated Ferroptosis in Bronchial Epithelial Cells Promotes Macrophage M2 Polarization in COPD Emphysema.

International journal of chronic obstructive pulmonary disease

Liu J,Zhang Z,Yang Y,Di T,Wu Y,Bian T

Fri Apr 08 00:00:00 UTC 2022

In-depth analysis of the expression and functions of signal transducers and activators of transcription in human ovarian cancer.

Frontiers in oncology

Gong X,Liu X

Thu Dec 22 00:00:00 UTC 2022

Tranexamic acid reduces endometrial cancer effects through the production of angiostatin.

Journal of Cancer

Hiramoto K,Yamate Y

Tue Apr 05 00:00:00 UTC 2022

Fusobacterium nucleatum exacerbates chronic obstructive pulmonary disease in elastase-induced emphysematous mice.

FEBS open bio

Suzuki R,Kamio N,Kaneko T,Yonehara Y,Imai K

Tue Mar 01 00:00:00 UTC 2022

Macrophage elastase (MMP12) critically contributes to the development of subretinal fibrosis.

Journal of neuroinflammation

Yi C,Liu J,Deng W,Luo C,Qi J,Chen M,Xu H

Tue Apr 05 00:00:00 UTC 2022

Matrix Metalloproteinase MMP-12 Promotes Macrophage Transmigration Across Intestinal Epithelial Tight Junctions and Increases Severity of Experimental Colitis.

Journal of Crohn's & colitis

Nighot M,Ganapathy AS,Saha K,Suchanec E,Castillo EF,Gregory A,Shapiro S,Ma T,Nighot P

Thu Oct 07 00:00:00 UTC 2021

Matrix Metalloproteinase MMP-12 Promotes Macrophage Transmigration Across Intestinal Epithelial Tight Junctions and Increases Severity of Experimental Colitis.

Journal of Crohn's & colitis

Nighot M,Ganapathy AS,Saha K,Suchanec E,Castillo EF,Gregory A,Shapiro S,Ma T,Nighot P

Thu Oct 07 00:00:00 UTC 2021

Metalloelastase-12 is involved in the temporomandibular joint inflammatory response as well as cartilage degradation by aggrecanases in STR/Ort mice.

Biomedical reports

Yamashita-Futani Y,Jokaji R,Ooi K,Kobayashi K,Kanakis I,Liu K,Kawashiri S,Bou-Gharios G,Nakamura H

Tue Jun 01 00:00:00 UTC 2021

Emerging Role of Dermal White Adipose Tissue in Modulating Hair Follicle Development During Aging.

Frontiers in cell and developmental biology

Chen J,Fan ZX,Zhu DC,Guo YL,Ye K,Dai D,Guo Z,Hu ZQ,Miao Y,Qu Q

Tue Nov 02 00:00:00 UTC 2021

MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.

Biomaterials

Song M,Zhang S,Tao Z,Li J,Shi Y,Xiong Y,Zhang W,Liu C,Chen S

Mon Nov 01 00:00:00 UTC 2021

MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.

Biomaterials

Song M,Zhang S,Tao Z,Li J,Shi Y,Xiong Y,Zhang W,Liu C,Chen S

Mon Nov 01 00:00:00 UTC 2021

MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.

Biomaterials

Song M,Zhang S,Tao Z,Li J,Shi Y,Xiong Y,Zhang W,Liu C,Chen S

Mon Nov 01 00:00:00 UTC 2021

MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.

Biomaterials

Song M,Zhang S,Tao Z,Li J,Shi Y,Xiong Y,Zhang W,Liu C,Chen S

Mon Nov 01 00:00:00 UTC 2021

Hydrogen sulfide attenuates cigarette smoke-induced airway remodeling by upregulating SIRT1 signaling pathway.

Redox biology

Guan R,Wang J,Cai Z,Li Z,Wang L,Li Y,Xu J,Li D,Yao H,Liu W,Deng B,Lu W

Wed Jan 01 00:00:00 UTC 2020

Severe elastolysis in hereditary gelsolin (AGel) amyloidosis.

Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis

Koskelainen S,Zhao F,Kalimo H,Baumann M,Kiuru-Enari S

Mon Jun 01 00:00:00 UTC 2020

Splenectomy enhances the Ly6C^low phenotype in hepatic macrophages by activating the ERK1/2 pathway during liver fibrosis.

International immunopharmacology

Zheng Z,Wang H,Li L,Zhang S,Zhang C,Zhang H,Ji F,Liu X,Zhu K,Kong G,Li Z

Tue Sep 01 00:00:00 UTC 2020

Andrographolide attenuates oxidative stress injury in cigarette smoke extract exposed macrophages through inhibiting SIRT1/ERK signaling.

International immunopharmacology

Zhang XF,Ding MJ,Cheng C,Zhang Y,Xiang SY,Lu J,Liu ZB

Wed Apr 01 00:00:00 UTC 2020

Matrix metalloprotease-mediated cleavage of neural glial-related cell adhesion molecules activates quiescent olfactory stem cells via EGFR.

Molecular and cellular neurosciences

Chen ZH,Luo XC,Yu CR,Huang L

Thu Oct 01 00:00:00 UTC 2020

Margatoxin mitigates CCl4‑induced hepatic fibrosis in mice via macrophage polarization, cytokine secretion and STAT signaling.

International journal of molecular medicine

Wu BM,Liu JD,Li YH,Li J

Wed Jan 01 00:00:00 UTC 2020

Margatoxin mitigates CCl4‑induced hepatic fibrosis in mice via macrophage polarization, cytokine secretion and STAT signaling.

International journal of molecular medicine

Wu BM,Liu JD,Li YH,Li J

Wed Jan 01 00:00:00 UTC 2020

Lung-derived HMGB1 is detrimental for vascular remodeling of metabolically imbalanced arterial macrophages.

Nature communications

Boytard L,Hadi T,Silvestro M,Qu H,Kumpfbeck A,Sleiman R,Fils KH,Alebrahim D,Boccalatte F,Kugler M,Corsica A,Gelb BE,Jacobowitz G,Miller G,Bellini C,Oakes J,Silvestre JS,Zangi L,Ramkhelawon B

Thu Aug 27 00:00:00 UTC 2020

Lung-derived HMGB1 is detrimental for vascular remodeling of metabolically imbalanced arterial macrophages.

Nature communications

Boytard L,Hadi T,Silvestro M,Qu H,Kumpfbeck A,Sleiman R,Fils KH,Alebrahim D,Boccalatte F,Kugler M,Corsica A,Gelb BE,Jacobowitz G,Miller G,Bellini C,Oakes J,Silvestre JS,Zangi L,Ramkhelawon B

Thu Aug 27 00:00:00 UTC 2020

Role of PM_2.5 in the development and progression of COPD and its mechanisms.

Respiratory research

Zhao J,Li M,Wang Z,Chen J,Zhao J,Xu Y,Wei X,Wang J,Xie J

Thu Jun 13 00:00:00 UTC 2019

Exosomal zinc transporter ZIP4 promotes cancer growth and is a novel diagnostic biomarker for pancreatic cancer.

Cancer science

Jin H,Liu P,Wu Y,Meng X,Wu M,Han J,Tan X

Sat Sep 01 00:00:00 UTC 2018

Exosomal zinc transporter ZIP4 promotes cancer growth and is a novel diagnostic biomarker for pancreatic cancer.

Cancer science

Jin H,Liu P,Wu Y,Meng X,Wu M,Han J,Tan X

Sat Sep 01 00:00:00 UTC 2018

The Role of Angiotensin II in Parietal Epithelial Cell Proliferation and Crescent Formation in Glomerular Diseases.

The American journal of pathology

Rizzo P,Novelli R,Rota C,Gagliardini E,Ruggiero B,Rottoli D,Benigni A,Remuzzi G

Wed Nov 01 00:00:00 UTC 2017

The Role of Angiotensin II in Parietal Epithelial Cell Proliferation and Crescent Formation in Glomerular Diseases.

The American journal of pathology

Rizzo P,Novelli R,Rota C,Gagliardini E,Ruggiero B,Rottoli D,Benigni A,Remuzzi G

Wed Nov 01 00:00:00 UTC 2017

Oridonin inhibits gefitinib-resistant lung cancer cells by suppressing EGFR/ERK/MMP-12 and CIP2A/Akt signaling pathways.

International journal of oncology

Xiao X,He Z,Cao W,Cai F,Zhang L,Huang Q,Fan C,Duan C,Wang X,Wang J,Liu Y

Wed Jun 01 00:00:00 UTC 2016

Oridonin inhibits gefitinib-resistant lung cancer cells by suppressing EGFR/ERK/MMP-12 and CIP2A/Akt signaling pathways.

International journal of oncology

Xiao X,He Z,Cao W,Cai F,Zhang L,Huang Q,Fan C,Duan C,Wang X,Wang J,Liu Y

Wed Jun 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: 3.4.24.65; Macrophage elastase; Macrophage metalloelastase; macrophage-metalloelastase; matrix metallo protease; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12; matrix metalloproteinase 12 (macrophage elastase); Matrix metalloproteinase-12; ME; MGC138506; MME; MMP; MMP-12; MMPs

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Gene Aliases: AV378681; HME; ME; MME; Mmel; MMP-12; MMP12

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UniProt ID: (Human) P39900, (Mouse) P34960, (Rat) Q63341

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Entrez Gene ID: (Human) 4321, (Mouse) 17381, (Rat) 117033

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Function(s)

endopeptidase activity metalloendopeptidase activity serine-type endopeptidase activity calcium ion binding zinc ion binding peptidase activity metallopeptidase activity hydrolase activity metal ion binding

Process(es)

proteolysis wound healing, spreading of epidermal cells wound healing positive regulation of epithelial cell proliferation involved in wound healing response to organic cyclic compound extracellular matrix disassembly collagen catabolic process response to drug positive regulation of epithelial cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MMP12 Polyclonal Antibody

MMP12 Antibody (22989-1-AP) in ICC/IF

Product Details

22989-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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