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Invitrogen
MMP13 Polyclonal Antibody
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MMP13 Antibody (PA5-95486) in IHC (P)
Immunohistochemistry analysis of MMP13 in paraffin-embedded mouse small intestine tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with MMP13 polyclonal antibody (Product # PA5-95486) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Comple... View More
Product Details
PA5-95486
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived rat MMP13 recombinant protein (Position: Y99-H335).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807288
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human A549 whole cell, human PC-3 whole cell, human U2OS whole cell, human HEK293 whole cell, rat testicular tissue, mouse testicular tissue. IHC: mouse lung tissue, mouse small intestine tissue, rat lung tissue, rat small intestine tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
MMP13 (collagense 3) plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC, and ACAN. MMP13 cleaves tripal helical collagens, including type I, II, and III collagen - but has the highest activity with soluble type II collagen. It can degrade collagen type IV, XIV, and X. MMP13 plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization, and ossification. It is required for normal embryonic bone development and ossificaiton. Mutations affecting the gene can lead to spondyloepimetaphyseal dysplasia Missouri type, metaphyseal anadysplasia 1, and metaphyseal dysplasia Sphar type.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Collagenase; Collagenase 3; collagenase-1; Collagenase-3; Collagenase3; interstitial collagenase; matrix metallo protease; matrix metalloproteinase 13; matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MMP; MMP-13; MMPs; Procollagenase 3; UMRCASE
Gene Aliases: Clg; CLG3; MANDP1; MMP-13; Mmp1; MMP13
UniProt ID: (Human) P45452, (Mouse) P33435
Entrez Gene ID: (Human) 4322, (Mouse) 17386, (Rat) 171052
fibronectin binding
metalloendopeptidase activity
serine-type endopeptidase activity
calcium ion binding
collagen binding
zinc ion binding
calcium-dependent protein binding
low-density lipoprotein particle receptor binding
peptidase activity
metallopeptidase activity
hydrolase activity
metal ion binding
endochondral ossification
growth plate cartilage development
proteolysis
extracellular matrix disassembly
bone mineralization
collagen catabolic process
receptor internalization
peptide catabolic process
cellular protein metabolic process
cartilage development
bone morphogenesis
ossification
luteolysis
response to hypoxia
response to mechanical stimulus
embryonic hindlimb morphogenesis
response to drug
response to estrogen
osteoblast differentiation
heart development
parturition
estrous cycle
cellular response to fluid shear stress
positive regulation of pancreatic trypsinogen secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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