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Invitrogen
MMP13 Recombinant Rabbit Monoclonal Antibody (JF0893)
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FIGURE: 1 / 8
MMP13 Antibody (MA5-42462) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of MMP13 in SW480 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with MMP13 Recombinant Monoclonal Antibody (Product # MA5-42462) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluor 594 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
Product Details
MA5-42462
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JF0893
Immunogen
Synthetic peptide within human MMP13 aa 390-430
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911603
Product Specific Information
Positive Control: SW480 cell lysate, MCF-7 cell lysate, Hela, MCF-7, SW480, human lung carcinoma tissue, human breast tissue, human breast carcinoma tissue.
Subcellular Location: Extracellular matrix, Secreted.
Target Information
MMP13 (collagense 3) plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC, and ACAN. MMP13 cleaves tripal helical collagens, including type I, II, and III collagen - but has the highest activity with soluble type II collagen. It can degrade collagen type IV, XIV, and X. MMP13 plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization, and ossification. It is required for normal embryonic bone development and ossificaiton. Mutations affecting the gene can lead to spondyloepimetaphyseal dysplasia Missouri type, metaphyseal anadysplasia 1, and metaphyseal dysplasia Sphar type.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Collagenase; Collagenase 3; Collagenase3; matrix metallo protease; matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MMP; MMP-13; MMPs; Procollagenase 3
Gene Aliases: CLG3; MANDP1; MMP-13; MMP13
UniProt ID: (Human) P45452
Entrez Gene ID: (Human) 4322
luteolysis
osteoblast differentiation
response to hypoxia
endochondral ossification
growth plate cartilage development
proteolysis
heart development
parturition
response to mechanical stimulus
extracellular matrix disassembly
bone mineralization
collagen catabolic process
embryonic hindlimb morphogenesis
response to drug
peptide catabolic process
response to estrogen
cellular protein metabolic process
estrous cycle
bone morphogenesis
cellular response to fluid shear stress
positive regulation of pancreatic trypsinogen secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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