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Invitrogen
MMP14 Recombinant Rabbit Monoclonal Antibody (6T7L3)
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FIGURE: 1 / 2
MMP14 Antibody (MA5-35044) in WB
Western blot analysis of MMP14 in extracts of various cell lines. Samples were incubated with MMP14 Monoclonal antibody (Product # MA5-35044) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
MA5-35044
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
6T7L3
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human MMP14/MMP14/MT1-MMP (P50281)
Conjugate
Form
Liquid
Concentration
0.67 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848949
Product Specific Information
Immunogen sequence: GAEIKANVRR KRYAIQGLKW QHNEITFCIQ NYTPKVGEYA TYEAIRKAFR VWESATPLRF REVPYAYIRE GHEKQADIMI FFAEGFHGDS TPFDGEGGFL A
Target Information
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 3.4.24.80; matrix metallo protease; matrix metallopeptidase 14 (membrane-inserted); matrix metalloproteinase 14 (membrane-inserted); matrix metalloproteinase 14 membrane-inserted; matrix metalloproteinase 14, membrane-inserted; Matrix metalloproteinase-14; membrane type 1 metalloprotease; membrane type 1-matrix metalloproteinase; Membrane type 1-MMP; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP; MMP-14; MMP-X MT-MMP; MMP-X1; MMPs; MT-MMP; MT-MMP 1; MT-MMP MT1-MMP; MT1-MMP; MT1MMP; MTMMP1; type 1 matrix metalloprotease 14
Gene Aliases: AI325305; MMP-14; MMP-X1; MMP14; MT-MMP; MT-MMP 1; MT-MMP-1; MT1-MMP; MT1MMP; Mtmmp; MTMMP1; sabe; WNCHRS
UniProt ID: (Human) P50281, (Mouse) P53690, (Rat) Q10739
Entrez Gene ID: (Human) 4323, (Mouse) 17387, (Rat) 81707
metalloendopeptidase activity
serine-type endopeptidase activity
integrin binding
calcium ion binding
protein binding
zinc ion binding
peptidase activator activity
transcription factor activity, sequence-specific DNA binding
peptidase activity
metallopeptidase activity
hydrolase activity
metal ion binding
ossification
endochondral ossification
regulation of transcription, DNA-templated
proteolysis
response to hormone
positive regulation of peptidase activity
cell migration
positive regulation of cell growth
lung development
positive regulation of cell migration
collagen catabolic process
zymogen activation
chondrocyte proliferation
embryonic cranial skeleton morphogenesis
branching morphogenesis of an epithelial tube
tissue remodeling
negative regulation of focal adhesion assembly
bone development
craniofacial suture morphogenesis
angiogenesis
ovarian follicle development
response to hypoxia
endothelial cell proliferation
response to oxidative stress
male gonad development
response to mechanical stimulus
response to organic cyclic compound
endodermal cell differentiation
astrocyte cell migration
response to estrogen
positive regulation of myotube differentiation
extracellular matrix disassembly
positive regulation of B cell differentiation
negative regulation of Notch signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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