Proteintech

MMP2 Monoclonal Antibody (2B10D1)

MMP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

MMP2 Antibody (66366-1-IG) in IHC (P)

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66366-1-IG (MMP2 antibody) at dilution of 1:300 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

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Product Details

66366-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 42 publications 42 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:150-1:600

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2B10D1

Immunogen

MMP2 Fusion Protein Ag25039 (34-469 aa encoded by BC002576)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: IIKFPGDVA PKTDKELAVQ YLNTFYGCPK ESCNLFVLKD TLKKMQKFFG LPQTGDLDQN TIETMRKPRC GNPDVANYNF FPRKPKWDKN QITYRIIGYT PDLDPETVDD AFARAFQVWS DVTPLRFSRI HDGEADIMIN FGRWEHGDGY PFDGKDGLLA HAFAPGTGVG GDSHFDDDEL WTLGEGQVVR VKYGNADGEY CKFPFLFNGK EYNSCTDTGR SDGFLWCSTT YNFEKDGKYG FCPHEALFTM GGNAEGQPCK FPFRFQGTSY DSCTTEGRTD GYRWCGTTED YDRDKKYGFC PETAMSTVGG NSEGAPCVFP FTFLGNKYES CTSAGRSDGK MWCATTANYD DDRKWGFCPD QGYSLFLVAA HEFGHAMGLE HSQDPGALMA PIYTYTKNFR LSQDDIKGIQ ELYGASPDID LGTGPTPTLG PVTPEIC (34-469 aa encoded by BC002576)

Target Information

MMP (matrix metalloproteinase) are proteolytic enzymes capable of degrading connective tissue components. MMP have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role in regulating angiogenesis. MMP2 is synthesized as a 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids, and contains the basic structure of propeptide, catalytic, and hemopexin domains. The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. Functionally, MMP2 is involved in tissue remodeling. Mutations in MMP-2 gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms of MMP-2 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 72 kDa gelatinase; 72 kDa type IV collagenase; 72kD gelatinase; 72kD type IV collagenase; 72kDa gelatinase; 72kDa type IV collagenase; CLG 4A; Collagenase; collagenase type IV-A; Gelatinase A; Gelatinase alpha; Mat; matrix metallo protease; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); matrix metalloproteinase 2; Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP; MMP-2; MMPs; neutrophil gelatinase; Progelatinase A; TBE-1; TBE1

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Gene Aliases: CLG4; CLG4A; GelA; MMP-2; MMP-II; MMP2; MONA; TBE-1

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UniProt ID: (Human) P08253, (Rat) P33436, (Mouse) P33434

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Entrez Gene ID: (Human) 4313, (Pig) 397391, (Rat) 81686, (Mouse) 17390

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Function(s)

metalloendopeptidase activity serine-type endopeptidase activity protein binding metallopeptidase activity zinc ion binding fibronectin binding peptidase activity hydrolase activity metal ion binding

Process(es)

angiogenesis response to hypoxia blood vessel maturation intramembranous ossification proteolysis embryo implantation extracellular matrix disassembly collagen catabolic process endodermal cell differentiation cellular protein metabolic process positive regulation of innate immune response ephrin receptor signaling pathway face morphogenesis bone trabecula formation cellular response to amino acid stimulus positive regulation of vascular smooth muscle cell proliferation ovarian follicle development ovulation from ovarian follicle luteinization response to oxidative stress negative regulation of cell adhesion female pregnancy parturition aging response to radiation response to mechanical stimulus peripheral nervous system axon regeneration response to activity cell migration positive regulation of cell migration response to retinoic acid response to cytokine response to nicotine response to drug response to hydrogen peroxide positive regulation of apoptotic process response to estrogen negative regulation of vasoconstriction skeletal system morphogenesis tissue remodeling response to electrical stimulus response to hyperoxia prostate gland epithelium morphogenesis cellular response to cytokine stimulus cellular response to interleukin-1 cellular response to estradiol stimulus cellular response to fluid shear stress

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MMP2 Monoclonal Antibody (2B10D1)

MMP2 Antibody (66366-1-IG) in IHC (P)

Product Details

66366-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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