Invitrogen

MMP2 Monoclonal Antibody (F14 P4 D3)

4 Published Figures

6 References

View all (44) MMP2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (4)

FIGURE: 1 / 6

MMP2 Antibody (MA1-772) in WB

Western blot analysis of MMP2 was performed by loading 25 µg of the indicated whole cell lysates and 10 µL Prestained Protein Ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. MMP2 was detected at ~74 kD using a MMP2 mouse monoclonal antibody (Product # MA1-772) at a concentration of 5 µg/mL in blocking buffer overnight at 4°C on a rocking platform, followed by... View More

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Product Details

MA1-772

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:500

View 5 publications 5 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

1-2 µg

Product Specifications

Species Reactivity

Human, Rat

Published species

Bovine, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

F14 P4 D3

Immunogen

Synthetic peptide corresponding to residues T(557) S L G L P P D V Q R V D(569) of human MMP-2.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_889473

Product Specific Information

MA1-772 contains 100 µg of in vitro produced, protein A purified antibody (1 mg/mL) in PBS containing 1 mg/mL BSA and 0.05% sodium azide.

MA1-772 detects MMP-2 from human and rat samples.

MA1-772 has been successfully used in Western blot applications. By Western blot, MA1-772 detects a ~50 kDa band representing MMP-2 from WI-38 cell lysate or rat lung samples.

The MA1-772 immunogen is a synthetic peptide corresponding to residues T(557) S L G L P P D V Q R V D(569) of human MMP-2.

MA1-772 has been successfully used in western blotting and ELISA analysis of MMP2 in human and rat samples.

By Western blot, MA1-772 detects a ~74 kDa band representing MMP2 in MCF7 and HT-1080 cell lysate or rat lung.

Target Information

MMP (matrix metalloproteinase) are proteolytic enzymes capable of degrading connective tissue components. MMP have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role in regulating angiogenesis. MMP2 is synthesized as a 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids, and contains the basic structure of propeptide, catalytic, and hemopexin domains. The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. Functionally, MMP2 is involved in tissue remodeling. Mutations in MMP-2 gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms of MMP-2 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Embryonic Trophectoderm Secretomics Reveals Chemotactic Migration and Intercellular Communication of Endometrial and Circulating MSCs in Embryonic Implantation.

International journal of molecular sciences

Calle A,Toribio V,Yáñez-Mó M,Ramírez MÁ

MA1-772 was used in Flow Cytometry to identify epithelial-mesenchymal transition stages using bovine blastocyst embryonic trophectoderm primary cultures.

Wed May 26 00:00:00 UTC 2021

Embryonic Trophectoderm Secretomics Reveals Chemotactic Migration and Intercellular Communication of Endometrial and Circulating MSCs in Embryonic Implantation.

International journal of molecular sciences

Calle A,Toribio V,Yáñez-Mó M,Ramírez MÁ

MA1-772 was used in Flow Cytometry to identify epithelial-mesenchymal transition stages using bovine blastocyst embryonic trophectoderm primary cultures.

Wed May 26 00:00:00 UTC 2021

miR‑205 suppresses cell migration, invasion and EMT of colon cancer by targeting mouse double minute 4.

Molecular medicine reports

Fan Y,Wang K

MA1-772 was used in Western Blotting to explore the effects of miR‚205 on human colon cancer and its targeting mechanism.

Sat Aug 01 00:00:00 UTC 2020

miR‑205 suppresses cell migration, invasion and EMT of colon cancer by targeting mouse double minute 4.

Molecular medicine reports

Fan Y,Wang K

MA1-772 was used in Western Blotting to explore the effects of miR‚205 on human colon cancer and its targeting mechanism.

Sat Aug 01 00:00:00 UTC 2020

Forkhead box C2 promotes the invasion ability of human trophoblast cells through Hedgehog (Hh) signaling pathway.

Cell biology international

Zhang Y,Zhang Y

Published figure using MMP2 monoclonal antibody (Product # MA1-772) in Western Blot

Sun Jul 01 00:00:00 UTC 2018

Silence of Stomatin-Like Protein 2 Represses Migration and Invasion Ability of Human Liver Cancer Cells via Inhibiting the Nuclear Factor Kappa B (NF-κB) Pathway.

Medical science monitor : international medical journal of experimental and clinical research

Zhu W,Li W,Geng Q,Wang X,Sun W,Jiang H,Pu X

MA1-772 was used in Western Blotting to study the potential roles and mechanisms of si-STOML2 in the migration and invasion of human hepatoma LM3 cells.

Thu Oct 25 00:00:00 UTC 2018

MicroRNA-27a Inhibits Cell Migration and Invasion of Fibroblast-Like Synoviocytes by Targeting Follistatin-Like Protein 1 in Rheumatoid Arthritis.

Molecules and cells

Shi DL,Shi GR,Xie J,Du XZ,Yang H

MA1-772 was used in western blot to investigate the effects of miR-27a on cell migration and invasion in fibroblast-like synoviocytes from rheumatoid arthritis patients

Wed Aug 31 00:00:00 UTC 2016

In vitro assays of rod and cone opsin activity: retinoid analogs as agonists and inverse agonists.

Methods in molecular biology (Clifton, N.J.)

Kono M,Crouch RK

MA1-772 was used in western blot to characterize rod and cone opsin activity using retinoid analogs

Thu Sep 09 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: 72 kDa gelatinase; 72 kDa type IV collagenase; CLG 4A; Collagenase; collagenase type IV-A; Gelatinase A; Gelatinase alpha; Mat; matrix metallo protease; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); matrix metalloproteinase 2; Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP; MMP-2; MMPs; neutrophil gelatinase; Progelatinase A; TBE-1; TBE1

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Gene Aliases: CLG4; CLG4A; MMP-2; MMP-II; MMP2; MONA; TBE-1

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UniProt ID: (Human) P08253, (Rat) P33436

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Entrez Gene ID: (Human) 4313, (Rat) 81686

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Function(s)

metalloendopeptidase activity serine-type endopeptidase activity protein binding metallopeptidase activity zinc ion binding fibronectin binding peptidase activity

Process(es)

angiogenesis response to hypoxia blood vessel maturation intramembranous ossification proteolysis embryo implantation extracellular matrix disassembly collagen catabolic process endodermal cell differentiation cellular protein metabolic process positive regulation of innate immune response ephrin receptor signaling pathway face morphogenesis bone trabecula formation cellular response to amino acid stimulus positive regulation of vascular smooth muscle cell proliferation ovarian follicle development ovulation from ovarian follicle luteinization response to oxidative stress negative regulation of cell adhesion female pregnancy parturition aging response to radiation response to mechanical stimulus peripheral nervous system axon regeneration response to activity cell migration positive regulation of cell migration response to retinoic acid response to cytokine response to nicotine response to drug response to hydrogen peroxide positive regulation of apoptotic process response to estrogen negative regulation of vasoconstriction skeletal system morphogenesis tissue remodeling response to electrical stimulus response to hyperoxia prostate gland epithelium morphogenesis cellular response to cytokine stimulus cellular response to interleukin-1 cellular response to estradiol stimulus cellular response to fluid shear stress

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MMP2 Monoclonal Antibody (F14 P4 D3)

MMP2 Antibody (MA1-772) in WB

Product Details

MA1-772

Western Blot (WB)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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