Proteintech

MMP9 Polyclonal Antibody

MMP9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MMP9 Antibody (27306-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human tonsilitis tissue slide using 27306-1-AP (MMP9 (Middle) antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MMP9 Antibody in Immunoprecipitation (IP)

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Product Details

27306-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:600

View 20 publications 20 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunoprecipitation (IP)

1:300

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MMP9 Fusion Protein Ag26132

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

267 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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IL-17A drives cognitive aging probably via inducing neuroinflammation and theta oscillation disruption in the hippocampus.

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CUX1 Facilitates the Development of Oncogenic Properties Via Activating Wnt/β-Catenin Signaling Pathway in Glioma.

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Therapeutic effect of neohesperidin on TNF-α-stimulated human rheumatoid arthritis fibroblast-like synoviocytes.

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Differentially expressed genes induced by β-caryophyllene in a rat model of cerebral ischemia-reperfusion injury.

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Bioinformatics

Protein Aliases: 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; Gelatinase-B; GELB; macrophage gelatinase; matrix metallo protease; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); Matrix metalloproteinase-9; Matrix metalloproteinase9; MMP; MMP-9; MMPs; type V collagenase

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Gene Aliases: CLG4B; GELB; MANDP2; MMP-9; MMP9

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UniProt ID: (Human) P14780

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Entrez Gene ID: (Human) 4318

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Function(s)

endopeptidase activity metalloendopeptidase activity serine-type endopeptidase activity protein binding collagen binding metallopeptidase activity zinc ion binding identical protein binding

Process(es)

skeletal system development ossification positive regulation of protein phosphorylation proteolysis embryo implantation extracellular matrix disassembly macrophage differentiation collagen catabolic process endodermal cell differentiation negative regulation of apoptotic process positive regulation of DNA binding positive regulation of epidermal growth factor receptor signaling pathway ephrin receptor signaling pathway leukocyte migration positive regulation of keratinocyte migration positive regulation of release of cytochrome c from mitochondria positive regulation of receptor binding positive regulation of vascular smooth muscle cell proliferation negative regulation of intrinsic apoptotic signaling pathway negative regulation of cation channel activity negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MMP9 Polyclonal Antibody

MMP9 Antibody (27306-1-AP) in IHC (P)

Product Details

27306-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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