Invitrogen

MMP9 Polyclonal Antibody

13 Published Figures

19 References

View all (62) MMP-9 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (13)

MMP9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 17

MMP9 Antibody (PA5-13199) in IHC (P)

Immunohistochemistry analysis of MMP9 in formalin-fixed and paraffin-embedded human cancer tissue. Samples were incubated with MMP9 polyclonal antibody (Product # PA5-13199) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepato... View More

MMP9 Antibody in Immunocytochemistry (ICC/IF)

MMP9 Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-13199

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 644-673 amino acids from the C-terminal region of human MMP9

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

Ammonium sulfate precipitation, Size-exclusion - Dialysis

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2144757

Target Information

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RAGE ablation attenuates glioma progression and enhances tumor immune responses by suppressing galectin-3 expression.

Neuro-oncology

Zhang IY,Liu S,Zhang L,Liang R,Fang Q,Zhao J,Ren L,Medina EF,Filippov A,Bonjoc KJ,Chaudhry A,Dayyani M,Bild AH,Badie B

PA5-13199 was used in Western Blot to support the development of RAGE ablation as a treatment strategy for malignant gliomas.

Thu May 04 00:00:00 UTC 2023

TRIM66 Promotes Malignant Progression of Non-Small-Cell Lung Cancer Cells via Targeting MMP9.

Computational and mathematical methods in medicine

Xu Y,Yang Q,Fang Z,Tan X,Zhang M,Chen W

Published figure using MMP9 polyclonal antibody (Product # PA5-13199) in Western Blot

Tue Aug 02 00:00:00 UTC 2022

Tofacitinib Inhibits STAT Phosphorylation and Matrix Metalloproteinase-3, -9 and -13 Production by C28/I2 Human Juvenile Chondrocytes.

Open access rheumatology : research and reviews

Thorpe JR,Wilson RA,Mesiano S,Malemud CJ

PA5-13199 was used in Immunohistochemistry (Paraffin), Western Blot to show ed that Tofacitinib, a pan-JAK small molecule inhibitor was a potent inhibitor of rhIL-6-induced STAT phosphorylation that appeared to be coupled to the inhibition of MMP-3, -9 and -13 production by C28/I2 chondrocytes.

Sun May 26 00:00:00 UTC 2024

Tofacitinib Inhibits STAT Phosphorylation and Matrix Metalloproteinase-3, -9 and -13 Production by C28/I2 Human Juvenile Chondrocytes.

Open access rheumatology : research and reviews

Thorpe JR,Wilson RA,Mesiano S,Malemud CJ

Sun May 26 00:00:00 UTC 2024

Acute Hyperglycemia Exacerbates Hemorrhagic Transformation after Embolic Stroke and Reperfusion with tPA: A Possible Role of TXNIP-NLRP3 Inflammasome.

Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association

Salman M,Ismael S,Li L,Ahmed HA,Puchowicz MA,Ishrat T

PA5-13199 was used in Western Blotting to suggest a potential role for TXNIP in the occurrence of HT in hyperglycemic conditions following eMCAO.

Tue Feb 01 00:00:00 UTC 2022

Neuroprotective effects of violacein in a model of inherited amyotrophic lateral sclerosis.

Scientific reports

Olivera-Bravo S,Bolatto C,Otero Damianovich G,Stancov M,Cerri S,Rodríguez P,Boragno D,Hernández Mir K,Cuitiño MN,Larrambembere F,Isasi E,Alem D,Canclini L,Marco M,Davyt D,Díaz-Amarilla P

PA5-13199 was used in Immunocytochemistry to study if the pigment violacein with several reported protective/antiproliferative properties may control highly neurotoxic astrocytes (AbAs) obtained from spinal cord cultures of symptomatic hSOD1G93A rats.

Tue Mar 15 00:00:00 UTC 2022

Hsa_circ_0074032 promotes prostate cancer progression through elevating homeobox A1 expression by serving as a microRNA-198 decoy.

Andrologia

Feng C,Wang Q,Deng L,Peng N,Yang M,Wang X

PA5-13199 was used in Immunohistochemistry (Paraffin), Western Blot to demonstrate that high circ_0074032 level in PCa is associated with worse PCa-related prognosis. Circ_0074032 induced cell apoptosis, curbed cell proliferation, migration and invasion in PCa cells and was identified as miR-198 decoy. Circ_0074032 regulates HOXA1 expression and promotes PCa progression via elevating HOXA1 expression through acting as a miR-198 sponge.

Tue Feb 01 00:00:00 UTC 2022

Transmembrane protein ADAM29 facilitates cell proliferation, invasion and migration in clear cell renal cell carcinoma.

Journal of chemotherapy (Florence, Italy)

Li SL,Jiang TQ,Cao QW,Liu SM

Published figure using MMP9 polyclonal antibody (Product # PA5-13199) in Western Blot

Mon Feb 01 00:00:00 UTC 2021

Endothelial Thioredoxin-Interacting Protein Depletion Reduces Hemorrhagic Transformation in Hyperglycemic Mice after Embolic Stroke and Thrombolytic Therapy.

Pharmaceuticals (Basel, Switzerland)

Salman M,Ismael S,Li L,Ahmed HA,Puchowicz MA,Ishrat T

PA5-13199 was used in Western Blot to test the hypothesis that endothelial-specific thioredoxin-interacting protein knock-out mice will be more resistant to the neurovascular damage associated with hyperglycemia in embolic stroke.

Mon Sep 27 00:00:00 UTC 2021

Endothelial Thioredoxin-Interacting Protein Depletion Reduces Hemorrhagic Transformation in Hyperglycemic Mice after Embolic Stroke and Thrombolytic Therapy.

Pharmaceuticals (Basel, Switzerland)

Salman M,Ismael S,Li L,Ahmed HA,Puchowicz MA,Ishrat T

Mon Sep 27 00:00:00 UTC 2021

Inhibition of Matrix Metalloproteinases and Cancer Cell Detachment by Ru(II) Polypyridyl Complexes Containing 4,7-Diphenyl-1,10-phenanthroline Ligands-New Candidates for Antimetastatic Agents.

Pharmaceuticals (Basel, Switzerland)

Gajda-Morszewski P,Gurgul I,Janczy-Cempa E,Mazuryk O,Łomzik M,Brindell M

PA5-13199 was used in Immunohistochemistry (Paraffin), Western Blot to show a reduction of metastatic cells escaping from the primary lesion to the surrounding tissue by prevention of their detachment and by influencing the activity of MMP-2 and MMP-9.

Fri Oct 01 00:00:00 UTC 2021

Inhibition of Matrix Metalloproteinases and Cancer Cell Detachment by Ru(II) Polypyridyl Complexes Containing 4,7-Diphenyl-1,10-phenanthroline Ligands-New Candidates for Antimetastatic Agents.

Pharmaceuticals (Basel, Switzerland)

Gajda-Morszewski P,Gurgul I,Janczy-Cempa E,Mazuryk O,Łomzik M,Brindell M

Fri Oct 01 00:00:00 UTC 2021

Ginsenoside Rg3 inhibits osteosarcoma progression by reducing circ_0003074 expression in a miR-516b-5p/KPNA4-dependent manner.

Journal of orthopaedic surgery and research

Wang T,Zhang C,Wang S

Published figure using MMP9 polyclonal antibody (Product # PA5-13199) in Western Blot

Mon Dec 20 00:00:00 UTC 2021

Cardiac Protective Effect of Kirenol against Doxorubicin-Induced Cardiac Hypertrophy in H9c2 Cells through Nrf2 Signaling via PI3K/AKT Pathways.