Invitrogen

MMP9 Polyclonal Antibody

2 Published Figures
5 References
View all (62) MMP-9 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-27191

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 2 publications 2 publications

Immunohistochemistry (IHC)

-
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

Immunomicroscopy (IM)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Bovine, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human MMP9. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.16 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2544667

Product Specific Information

Recommended positive controls: MCF-7 (starvation, 200 nM PMA treatment for 24 hr, and 5 µg/mL Brefeldin A treatment for 14.5 hr), Huh7.

Predicted reactivity: Mouse (82%), Rat (84%), Dog (81%), Pig (84%), Rabbit (89%), Rhesus Monkey (96%), Bovine (85%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 92 kDa gelatinase; 92 kDa type IV collagenase; 92-kDa type IV collagenase; 92kD gelatinase; 92kD type IV collagenase; 92kDa gelatinase; 92kDa type IV collagenase; collagenase type IVB; Gel B; Gelatinase B; Gelatinase-B; GELB; macrophage gelatinase; matrix metallo protease; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9; matrix metalloproteinase 9 (gelatinase B 92-kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92kDa matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); Matrix metalloproteinase-9; Matrix metalloproteinase9; MMP; MMP-9; MMPs; type V collagenase

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Gene Aliases: AW743869; B/MMP9; BOS_13297; CLG4B; GELB; MANDP2; MMP-9; MMP9; pro-MMP-9

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UniProt ID: (Bovine) P52176, (Human) P14780, (Rat) P50282, (Mouse) P41245

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Entrez Gene ID: (Bovine) 282871, (Human) 4318, (Rat) 81687, (Mouse) 17395

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Function(s)
metalloendopeptidase activity zinc ion binding endopeptidase activity serine-type endopeptidase activity protein binding collagen binding metallopeptidase activity identical protein binding fibronectin binding peptidase activity protein complex binding hydrolase activity metal ion binding
Process(es)
proteolysis collagen catabolic process skeletal system development ossification response to hypoxia kidney development positive regulation of leukocyte migration response to oxidative stress embryo implantation parturition aging response to nutrient response to radiation response to heat response to mechanical stimulus response to high light intensity response to organic substance response to organic cyclic compound transformation of host cell by virus extracellular matrix organization positive regulation of synaptic plasticity response to estradiol response to lipopolysaccharide response to retinoic acid response to vitamin A response to tumor necrosis factor response to nicotine endodermal cell differentiation response to drug positive regulation of apoptotic process response to ethanol positive regulation of angiogenesis negative regulation of fibroblast proliferation tissue remodeling leukocyte migration protein oligomerization positive regulation of keratinocyte migration response to hyperoxia cellular response to lipopolysaccharide cellular response to iron(III) ion cellular response to cytokine stimulus cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to low-density lipoprotein particle stimulus cellular response to cell-matrix adhesion positive regulation of receptor binding negative regulation of cation channel activity positive regulation of protein phosphorylation negative regulation of apoptotic process positive regulation of DNA binding positive regulation of epidermal growth factor receptor signaling pathway positive regulation of release of cytochrome c from mitochondria negative regulation of intrinsic apoptotic signaling pathway negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway extracellular matrix disassembly macrophage differentiation ephrin receptor signaling pathway positive regulation of vascular smooth muscle cell proliferation
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