Invitrogen

MMP9 Monoclonal Antibody (5G3)

MMP9 Antibody in Immunocytochemistry (ICC/IF)

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MMP9 Antibody (MA5-15886) in ICC/IF

Immunofluorescence analysis of Matrix metalloproteinase-9 was performed using 70% confluent log phase THP-1 cells differentiated to Macrophages using PMA (100nM 48 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MMP9 Monoclonal Antibody (5G3) (Product # MA5-15886) at 1:500 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+... View More

MMP9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MMP9 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

MMP9 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. THP-1 cells were transfected with Matrix metalloproteinase-9 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-MMP9 Monoclonal Antibody (5G3) (Product # MA5-15886). {KD}

Product Details

MA5-15886

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 18 publications 18 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:200-1:1,000

View 3 publications 3 publications

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

Mass Spectrometry (MS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

5G3

Immunogen

Purified recombinant fragment of human MMP9 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11157246

Product Specific Information

MA5-15886 targets MMP9 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15886 immunogen is purified recombinant fragment of human MMP9 expressed in E. Coli. .

MA5-15886 detects MMP9 which has a predicted molecular weight of approximately 92kDa.

Target Information

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Scar-Degrading Endothelial Cells as a Treatment for Advanced Liver Fibrosis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhao P,Sun T,Lyu C,Liang K,Niu Y,Zhang Y,Cao C,Xiang C,Du Y

Published figure using MMP9 monoclonal antibody (Product # MA5-15886) in Western Blot

Wed Feb 01 00:00:00 UTC 2023

Scar-Degrading Endothelial Cells as a Treatment for Advanced Liver Fibrosis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhao P,Sun T,Lyu C,Liang K,Niu Y,Zhang Y,Cao C,Xiang C,Du Y

Published figure using MMP9 monoclonal antibody (Product # MA5-15886) in Western Blot

Wed Feb 01 00:00:00 UTC 2023

Scar-Degrading Endothelial Cells as a Treatment for Advanced Liver Fibrosis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhao P,Sun T,Lyu C,Liang K,Niu Y,Zhang Y,Cao C,Xiang C,Du Y

Published figure using MMP9 monoclonal antibody (Product # MA5-15886) in Western Blot

Wed Feb 01 00:00:00 UTC 2023

EIF5A2 Is Involved in the Biological Process of Cervical Cancer Cells through AGR2.

Pharmacology

Shen X,Li L,He Y,Lv X,Ma J

MA5-15886 was used in Western Blotting to show that EIF5A2 knockdown inhibited the biological process of cervical cancer cells through modulation of AGR2.

Wed Jul 06 00:00:00 UTC 2022

Glucosidase inhibitor, Nimbidiol ameliorates renal fibrosis and dysfunction in type-1 diabetes.

Scientific reports

Juin SK,Pushpakumar S,Tyagi SC,Sen U

MA5-15886 was used in Western Blotting to suggest that Nimbidiol attenuates inflammation and ECM accumulation and thereby, protects kidney from fibrosis and dysfunction possibly by inhibiting TGF-β/Smad and MAPK signaling pathways in type-1 diabetes.

Thu Dec 15 00:00:00 UTC 2022

Glucosidase inhibitor, Nimbidiol ameliorates renal fibrosis and dysfunction in type-1 diabetes.

Scientific reports

Juin SK,Pushpakumar S,Tyagi SC,Sen U

Thu Dec 15 00:00:00 UTC 2022

Silencing of circCYP51A1 represses cell progression and glycolysis by regulating miR-490-3p/KLF12 axis in osteosarcoma under hypoxia.

Journal of bone oncology

Yang J,Liu Z,Liu B,Sun L

Published figure using MMP9 monoclonal antibody (Product # MA5-15886) in Immunohistochemistry

Thu Dec 01 00:00:00 UTC 2022

Methamphetamine induces transcriptional changes in cultured HIV-infected mature monocytes that may contribute to HIV neuropathogenesis.

Frontiers in immunology

Chilunda V,Weiselberg J,Martinez-Meza S,Mhamilawa LE,Cheney L,Berman JW

MA5-15886 was used in Western Blotting to highlight potential molecules that may be targeted for therapy to limit the effects of meth on HIV neuropathogenesis.

Thu Sep 08 00:00:00 UTC 2022

Methamphetamine induces transcriptional changes in cultured HIV-infected mature monocytes that may contribute to HIV neuropathogenesis.

Frontiers in immunology

Chilunda V,Weiselberg J,Martinez-Meza S,Mhamilawa LE,Cheney L,Berman JW

MA5-15886 was used in Western Blotting to highlight potential molecules that may be targeted for therapy to limit the effects of meth on HIV neuropathogenesis.

Thu Sep 08 00:00:00 UTC 2022

TGF-β1/SH2B3 axis regulates anoikis resistance and EMT of lung cancer cells by modulating JAK2/STAT3 and SHP2/Grb2 signaling pathways.

Cell death & disease

Wang LN,Zhang ZT,Wang L,Wei HX,Zhang T,Zhang LM,Lin H,Zhang H,Wang SQ

Published figure using MMP9 monoclonal antibody (Product # MA5-15886) in Western Blot

Thu May 19 00:00:00 UTC 2022

Hsa_circ_0134111 promotes intervertebral disc degeneration via sponging miR-578.

Cell death discovery

Yan P,Sun C,Luan L,Han J,Qu Y,Zhou C,Xu D

Published figure using MMP9 monoclonal antibody (Product # MA5-15886) in Western Blot

Tue Feb 08 00:00:00 UTC 2022

Hsa_circ_0134111 promotes intervertebral disc degeneration via sponging miR-578.

Cell death discovery

Yan P,Sun C,Luan L,Han J,Qu Y,Zhou C,Xu D

Published figure using MMP9 monoclonal antibody (Product # MA5-15886) in Western Blot

Tue Feb 08 00:00:00 UTC 2022

Antitumor Potential of Sericite Treatment Mediated by Cell Cycle Arrest in Triple-Negative MDA-MB231 Breast Cancer Cells.

Evidence-based complementary and alternative medicine : eCAM

Kim S,Nagar H,Lee I,Choi SJ,Piao S,Jeon BH,Kang SK,Song HJ,Kim CS

Published figure using MMP9 monoclonal antibody (Product # MA5-15886) in Western Blot

Fri Oct 07 00:00:00 UTC 2022

Ozone combined with doxorubicin exerts cytotoxic and anticancer effects on Luminal-A subtype human breast cancer cell line.

Revista da Associacao Medica Brasileira (1992)

Karagulle OO,Yurttas AG

MA5-15886 was used in Immunocytochemistry to examine the potential anticancer effects of ozone applied after chemotherapeutic treatment with different concentrations of doxorubicin in Luminal-A subtype of human breast cancer cell line (MCF-7) and compare the results with effects on L929 fibroblast cell line.

Fri Apr 01 00:00:00 UTC 2022

Testicular orphan receptor 4 (TR4) promotes papillary thyroid cancer invasion via activating circ-FNLA/miR-149-5p/MMP9 signaling.

Molecular therapy. Nucleic acids

Ouyang X,Feng L,Yao L,Xiao Y,Hu X,Zhang G,Liu G,Wang Z

MA5-15886 was used in Western Blot to investigate the oncogenic role of testicular orphan receptor 4 in papillary thyroid cancer development.

Fri Jun 04 00:00:00 UTC 2021

Testicular orphan receptor 4 (TR4) promotes papillary thyroid cancer invasion via activating circ-FNLA/miR-149-5p/MMP9 signaling.

Molecular therapy. Nucleic acids

Ouyang X,Feng L,Yao L,Xiao Y,Hu X,Zhang G,Liu G,Wang Z

MA5-15886 was used in Western Blot to investigate the oncogenic role of testicular orphan receptor 4 in papillary thyroid cancer development.

Fri Jun 04 00:00:00 UTC 2021

Exogenous hydrogen sulfide and miR-21 antagonism attenuates macrophage-mediated inflammation in ischemia reperfusion injury of the aged kidney.

GeroScience

Pushpakumar S,Kundu S,Weber G,Sen U

MA5-15886 was used in Western Blot to determine the effects of age-induced reduction in hydrogen sulfide and mir-21 antagonism in acute kidney injury.

Tue Jun 01 00:00:00 UTC 2021

Silencing of hsa_circ_0009035 Suppresses Cervical Cancer Progression and Enhances Radiosensitivity through MicroRNA 889-3p-Dependent Regulation of HOXB7.

Molecular and cellular biology

Zhao X,Dong W,Luo G,Xie J,Liu J,Yu F

MA5-15886 was used in Western Blotting to investigate circular RNAs and microRNAs influencing HoXB7 in cervical cancer.

Fri May 21 00:00:00 UTC 2021

MUC 15 Promotes Osteosarcoma Cell Proliferation, Migration and Invasion through Livin, MMP-2/MMP-9 and Wnt/β-Catenin Signal Pathway.

Journal of Cancer

Chen T,Chen Z,Lian X,Wu W,Chu L,Zhang S,Wang L

MA5-15886 was used in Western Blotting to investigate the high expression of MUC15 in promoting proliferation, migration and invasion in osteosarcoma cells and its potential mechanism.

Tue Jan 05 00:00:00 UTC 2021

MUC 15 Promotes Osteosarcoma Cell Proliferation, Migration and Invasion through Livin, MMP-2/MMP-9 and Wnt/β-Catenin Signal Pathway.

Journal of Cancer

Chen T,Chen Z,Lian X,Wu W,Chu L,Zhang S,Wang L

MA5-15886 was used in Western Blotting to investigate the high expression of MUC15 in promoting proliferation, migration and invasion in osteosarcoma cells and its potential mechanism.

Tue Jan 05 00:00:00 UTC 2021

Identification of circ_0058357 as a regulator in non-small cell lung cancer cells resistant to cisplatin by miR-361-3p/ABCC1 axis.

Thoracic cancer

Chu D,Li P,Li Y,Shi J,Huang S,Jiao P

MA5-15886 was used in Immunohistochemistry-immunofluorescence to identify that inhibition of circ_0058357 suppresses the growth and metastasis of H1299/DDP and A549/DDP cells and sensitizes them to DDP therapy partially by targeting the miR-361-3p/ABCC1 axis.

Mon Nov 01 00:00:00 UTC 2021

Identification of circ_0058357 as a regulator in non-small cell lung cancer cells resistant to cisplatin by miR-361-3p/ABCC1 axis.

Thoracic cancer

Chu D,Li P,Li Y,Shi J,Huang S,Jiao P

Mon Nov 01 00:00:00 UTC 2021

GYY4137 Regulates Extracellular Matrix Turnover in the Diabetic Kidney by Modulating Retinoid X Receptor Signaling.

Biomolecules

Juin SK,Pushpakumar S,Sen U

MA5-15886 was used in Western Blot to suggest that RXR signaling plays a significant role in ECM turnover, and GYY4137 modulates PPAR/RAR-mediated RXR signaling to ameliorate PAI-1-dependent adverse ECM turnover in DN.

Thu Oct 07 00:00:00 UTC 2021

GYY4137 Regulates Extracellular Matrix Turnover in the Diabetic Kidney by Modulating Retinoid X Receptor Signaling.

Biomolecules

Juin SK,Pushpakumar S,Sen U

Thu Oct 07 00:00:00 UTC 2021

Adiponectin and Leptin Exert Antagonizing Effects on HUVEC Tube Formation and Migration Modulating the Expression of CXCL1, VEGF, MMP-2 and MMP-9.

International journal of molecular sciences

Nigro E,Mallardo M,Polito R,Scialò F,Bianco A,Daniele A

MA5-15886 was used in Western Blotting to investigate the effects following the treatment of primary human umbilical vein endothelial cells with AdipoRon and/or leptin on proliferation, angiogenesis, and wound healing.

Tue Jul 13 00:00:00 UTC 2021

Adiponectin and Leptin Exert Antagonizing Effects on HUVEC Tube Formation and Migration Modulating the Expression of CXCL1, VEGF, MMP-2 and MMP-9.

International journal of molecular sciences

Nigro E,Mallardo M,Polito R,Scialò F,Bianco A,Daniele A

Tue Jul 13 00:00:00 UTC 2021

Sevoflurane inhibits the proliferation and invasion of hepatocellular carcinoma cells through regulating the PTEN/Akt/GSK‑3β/β‑catenin signaling pathway by downregulating miR‑25‑3p.

International journal of molecular medicine

Cao Y,Lv W,Ding W,Li J

MA5-15886 was used in Western Blotting to determine the molecular mechanisms governing sevoflurane's action in hepatocellular carcinoma cells.

Wed Jul 01 00:00:00 UTC 2020

Sevoflurane inhibits the proliferation and invasion of hepatocellular carcinoma cells through regulating the PTEN/Akt/GSK‑3β/β‑catenin signaling pathway by downregulating miR‑25‑3p.

International journal of molecular medicine

Cao Y,Lv W,Ding W,Li J

MA5-15886 was used in Western Blotting to determine the molecular mechanisms governing sevoflurane's action in hepatocellular carcinoma cells.

Wed Jul 01 00:00:00 UTC 2020

miR-939-3p promotes epithelial-mesenchymal transition and may be used as a prognostic marker in hepatocellular carcinoma.

Oncology letters

Chen F,Ni X,Chen L,Wang X,Xu J

Published figure using MMP9 monoclonal antibody (Product # MA5-15886) in Western Blot

Wed Apr 01 00:00:00 UTC 2020

Hydrogen sulphide mitigates homocysteine-induced apoptosis and matrix remodelling in mesangial cells through Akt/FOXO1 signalling cascade.

Cellular signalling

Majumder S,Ren L,Pushpakumar S,Sen U

MA5-15886 was used in Western Blot to conclude that mesangial cell damage and extracellular remodelling caused by hyperhomocysteinemia is regulated by the Akt/FOXO1 pathway.

Sun Sep 01 00:00:00 UTC 2019

MMP-2 and MMP-9 as prognostic markers for the early detection of urinary bladder cancer.

Journal of biochemical and molecular toxicology

Fouad H,Salem H,Ellakwa DE,Abdel-Hamid M

Published figure using MMP9 monoclonal antibody (Product # MA5-15886) in Western Blot

Mon Apr 01 00:00:00 UTC 2019

Characterization of metastatic tumor antigen 1 and its interaction with hepatitis B virus X protein in NF-κB signaling and tumor progression in a woodchuck hepatocellular carcinoma model.

Oncotarget

Li YT,Liu CJ,Su TH,Cheng HR,Jeng YM,Lin HL,Wang CC,Kao JH,Chen PJ,Chen DS,Wu HL

MA515886 was used in western blot to explore the role of metastatic tumor antigen 1 in hepatitis B-associated hepatocarcinogenesis in the woodchuck model

Tue Jul 26 00:00:00 UTC 2016

An essential role of syntaxin 3 protein for granule exocytosis and secretion of IL-1α, IL-1β, IL-12b, and CCL4 from differentiated HL-60 cells.

Journal of leukocyte biology

Naegelen I,Plançon S,Nicot N,Kaoma T,Muller A,Vallar L,Tschirhart EJ,Bréchard S

MA5-15886 was used in immunocytochemistry to elucidate the intracellular SNARE machinery responsible for the regulation of cytokine secretion and degranulation.

Sun Mar 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: 92 kDa gelatinase; 92 kDa type IV collagenase; 92kD gelatinase; 92kD type IV collagenase; 92kDa gelatinase; 92kDa type IV collagenase; collagenase type IVB; Gel B; Gelatinase B; Gelatinase-B; GELB; macrophage gelatinase; matrix metallo protease; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); Matrix metalloproteinase-9; Matrix metalloproteinase9; MMP; MMP-9; MMPs; type V collagenase

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Gene Aliases: AW743869; B/MMP9; CLG4B; GELB; MANDP2; MMP-9; MMP9; pro-MMP-9

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UniProt ID: (Human) P14780, (Mouse) P41245

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Entrez Gene ID: (Human) 4318, (Mouse) 17395

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Function(s)

endopeptidase activity metalloendopeptidase activity serine-type endopeptidase activity protein binding collagen binding metallopeptidase activity zinc ion binding identical protein binding fibronectin binding peptidase activity hydrolase activity protein complex binding metal ion binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MMP9 Monoclonal Antibody (5G3)

MMP9 Antibody (MA5-15886) in ICC/IF

Product Details

MA5-15886

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Mass Spectrometry (MS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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