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MPHOSPH1 Antibody (PA5-120351) in WB
Western blot analysis of MPHOSPH1 in extracts of various cell lines. Samples were incubated with MPHOSPH1 Polyclonal antibody (Product # PA5-120351) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-120351
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1500 of human KIF20B (NP_057279.2).
Conjugate
Form
Liquid
Concentration
0.53 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913923
Product Specific Information
Positive test controls include: U-251MG, OVCAR3, 293T, HT-29, NIH/3T3, Mouse brain, Rat brain. The target is usually found in the following locations: Cell projection, Cytoplasm, Midbody, Nucleus, axon, centrosome, cytoskeleton, growth cone, microtubule organizing center, nucleolus, nucleoplasm, spindle, spindle pole.
Immunogen sequence: KHLLQLKEEE EETNRQETEK LKEELSASSA RTQNLKADLQ RKEEDYADLK EKLTDAKKQI KQVQKEVSVM RDEDKLLRIK INELEKKKNQ CSQELDMKQR TIQQLKEQLN NQKVEEAIQQ YERACKDLNV KEKIIEDMRM TLEEQEQTQV EQDQVLEAKL EEVERLATEL EKWKEKCNDL ETKNNQRSNK EHENNTDVLG KLTNLQDELQ ESEQKYNADR KKWLEEKMML ITQAKEAENI RNKEMKKYAE DRERFFKQQN EMEILTAQLT EKDSDLQKWR EERDQLVAAL EIQLKALISS N
Target Information
KIF20B is a plus-end-directed motor enzyme that is required for completion of cytokinesis.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cancer/testis antigen 90; CT90; Kinesin family member 20B; Kinesin-like protein KIF20B; Kinesin-related motor interacting with PIN1; M-phase phosphoprotein 1; mitotic kinesin-like protein; mitotic kinesin-related protein; MPP1; N-6 kinesin
Gene Aliases: 33cex; B130024C23; C330014J10Rik; CT90; KIF20B; KRMP1; magoo; MPHOSPH1; MPP-1; MPP1
UniProt ID: (Human) Q96Q89, (Mouse) Q80WE4
Entrez Gene ID: (Human) 9585, (Mouse) 240641, (Rat) 309523
neural tube closure
microtubule-based movement
cell cycle
mitotic nuclear division
regulation of mitotic nuclear division
metabolic process
cell division
regulation of establishment of protein localization
regulation of establishment of cell polarity
regulation of neuron migration
cell cycle arrest
positive regulation of cell proliferation
positive regulation of cytokinesis
protein localization to microtubule
neuron projection morphogenesis
positive regulation of intracellular protein transport
positive regulation of mitotic cytokinetic process
positive regulation of neuron migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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