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Invitrogen
MPI Polyclonal Antibody
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MPI Antibody (PA5-95402) in IHC (P)
Immunohistochemical analysis of MPI in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-MPI antibody (Product # PA5-95402) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as ... View More
Product Details
PA5-95402
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human MPI recombinant protein (Position: A2-K99).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2807205
Product Specific Information
Human MPI shares 88.8% and 86.7% amino acid (aa) sequence identity with mouse and rat MPI, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human Caco-2 whole cell, human HEK293 whole cell, rat brain tissue, rat lung tissue, rat heart tissue, mouse brain tissue, mouse lung tissue. IHC: human intestinal cancer tissue, human lung cancer tissue, rat brain tissue. Flow: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: mannose phosphate isomerase 1; Mannose-6-phosphate isomerase; Phosphohexomutase; Phosphomannose isomerase; phosphomannose isomerase 1; PMI
Gene Aliases: 1110002E17Rik; AI315153; CDG1B; MPI; Mpi-1; Mpi1; PMI; PMI1
UniProt ID: (Human) P34949, (Rat) Q68FX1, (Mouse) Q924M7
Entrez Gene ID: (Human) 4351, (Rat) 300741, (Mouse) 110119
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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