Proteintech

MPP8 Polyclonal Antibody

FIGURE: 1 / 7

MPP8 Antibody (16796-1-AP) in WB

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 16796-1-AP (MPP8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

MPP8 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16796-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 4 publications 4 publications

ChIP assay (ChIP)

View 3 publications 3 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MPP8 Fusion Protein Ag10268 (1-301 aa encoded by BC003542)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MEQVAEGAR VTAVPVSAAD STEELAEVEE GVGVVGEDND AAARGAEAFG DSEEDGEDVF EVEKILDMKT EGGKVLYKVR WKGYTSDDDT WEPEIHLEDC KEVLLEFRKK IAENKAKAVR KDIQRLSLNN DIFEANSDSD QQSETKEDTS PKKKKKKLRQ REEKSPDDLK KKKAKAGKLK DKSKPDLESS LESLVFDLRT KKRISEAKEE LKESKKPKKD EVKETKELKK VKKGEIRDLK TKTREDPKEN RKTKKEKFVE SQVESESSVL NDSPFPEDDS EGLHSDSREE KQNTKSARER AG (1-301 aa encoded by BC003542)

Target Information

Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2 (PubMed:26022416, PubMed:28581500). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592). Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression (PubMed:29211708). The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed (PubMed:30487602). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Co-transcriptional genome surveillance by HUSH is coupled to termination machinery.

Molecular cell

Spencley AL,Bar S,Swigut T,Flynn RA,Lee CH,Chen LF,Bassik MC,Wysocka J

Thu May 18 00:00:00 UTC 2023

Characterization of Hepatoma-Derived Growth Factor-Related Protein 2 Interactions with Heterochromatin.

Cells

Wistner SC,MacDonald IA,Stanley KA,Hathaway NA

Sat Jan 14 00:00:00 UTC 2023

Epigenetic Silencing of Recombinant Adeno-associated Virus Genomes by NP220 and the HUSH Complex.

Journal of virology

Das A,Vijayan M,Walton EM,Stafford VG,Fiflis DN,Asokan A

Wed Feb 23 00:00:00 UTC 2022

Discovery of Potent Peptidomimetic Antagonists for Heterochromatin Protein 1 Family Proteins.

ACS omega

Lamb KN,Dishman SN,Waybright JM,Engelberg IA,Rectenwald JM,Norris-Drouin JL,Cholensky SH,Pearce KH,James LI,Frye SV

Tue Jan 11 00:00:00 UTC 2022

Chromatin modifier HUSH co-operates with RNA decay factor NEXT to restrict transposable element expression.

Molecular cell

Garland W,Müller I,Wu M,Schmid M,Imamura K,Rib L,Sandelin A,Helin K,Jensen TH

Thu May 05 00:00:00 UTC 2022

Chromatin modifier HUSH co-operates with RNA decay factor NEXT to restrict transposable element expression.

Molecular cell

Garland W,Müller I,Wu M,Schmid M,Imamura K,Rib L,Sandelin A,Helin K,Jensen TH

Thu May 05 00:00:00 UTC 2022

Genome surveillance by HUSH-mediated silencing of intronless mobile elements.

Nature

Seczynska M,Bloor S,Cuesta SM,Lehner PJ

Sat Jan 01 00:00:00 UTC 2022

Silencing of LINE-1 retrotransposons is a selective dependency of myeloid leukemia.

Nature genetics

Gu Z,Liu Y,Zhang Y,Cao H,Lyu J,Wang X,Wylie A,Newkirk SJ,Jones AE,Lee M,Botten GA,Deng M,Dickerson KE,Zhang CC,An W,Abrams JM,Xu J

Sat May 01 00:00:00 UTC 2021

A Peptidomimetic Ligand Targeting the Chromodomain of MPP8 Reveals HRP2's Association with the HUSH Complex.

ACS chemical biology

Waybright JM,Clinkscales SE,Barnash KD,Budziszewski GR,Rectenwald JM,Chiarella AM,Norris-Drouin JL,Cholensky SH,Pearce KH,Herring LE,McGinty RK,Hathaway NA,James LI

Fri Sep 17 00:00:00 UTC 2021

MPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells.

Nature communications

Müller I,Moroni AS,Shlyueva D,Sahadevan S,Schoof EM,Radzisheuskaya A,Højfeldt JW,Tatar T,Koche RP,Huang C,Helin K

Mon May 24 00:00:00 UTC 2021

MPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells.

Nature communications

Müller I,Moroni AS,Shlyueva D,Sahadevan S,Schoof EM,Radzisheuskaya A,Højfeldt JW,Tatar T,Koche RP,Huang C,Helin K

Mon May 24 00:00:00 UTC 2021

The HUSH complex is a gatekeeper of type I interferon through epigenetic regulation of LINE-1s.

Nature communications

Tunbak H,Enriquez-Gasca R,Tie CHC,Gould PA,Mlcochova P,Gupta RK,Fernandes L,Holt J,van der Veen AG,Giampazolias E,Burns KH,Maillard PV,Rowe HM

Tue Nov 03 00:00:00 UTC 2020

M-phase phosphoprotein 8 promotes gastric cancer growth and metastasis via p53/Bcl-2 and EMT-related signaling pathways.

Journal of cellular biochemistry

Wang Y,Xiao H,Wang C,Wu H,He H,Yao C,Cui J,Li W

Sun Mar 01 00:00:00 UTC 2020

TASOR is a pseudo-PARP that directs HUSH complex assembly and epigenetic transposon control.

Nature communications

Douse CH,Tchasovnikarova IA,Timms RT,Protasio AV,Seczynska M,Prigozhin DM,Albecka A,Wagstaff J,Williamson JC,Freund SMV,Lehner PJ,Modis Y

Fri Oct 02 00:00:00 UTC 2020

Comprehensive nucleosome interactome screen establishes fundamental principles of nucleosome binding.

Nucleic acids research

Skrajna A,Goldfarb D,Kedziora KM,Cousins EM,Grant GD,Spangler CJ,Barbour EH,Yan X,Hathaway NA,Brown NG,Cook JG,Major MB,McGinty RK

Fri Sep 25 00:00:00 UTC 2020

Pathway-Based High-Throughput Chemical Screen Identifies Compounds That Decouple Heterochromatin Transformations.

SLAS discovery : advancing life sciences R & D

MacDonald IA,Butler KV,Herring LE,Clinkscales SE,Yelagandula R,Stecher K,Bell O,Graves LM,Jin J,Hathaway NA

Sun Sep 01 00:00:00 UTC 2019

The H3K9 Methylation Writer SETDB1 and its Reader MPP8 Cooperate to Silence Satellite DNA Repeats in Mouse Embryonic Stem Cells.

Genes

Cruz-Tapias P,Robin P,Pontis J,Maestro LD,Ait-Si-Ali S

Wed Sep 25 00:00:00 UTC 2019

The H3K9 Methylation Writer SETDB1 and its Reader MPP8 Cooperate to Silence Satellite DNA Repeats in Mouse Embryonic Stem Cells.

Genes

Cruz-Tapias P,Robin P,Pontis J,Maestro LD,Ait-Si-Ali S

Wed Sep 25 00:00:00 UTC 2019

The H3K9 Methylation Writer SETDB1 and its Reader MPP8 Cooperate to Silence Satellite DNA Repeats in Mouse Embryonic Stem Cells.

Genes

Cruz-Tapias P,Robin P,Pontis J,Maestro LD,Ait-Si-Ali S

Wed Sep 25 00:00:00 UTC 2019

Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex.

Nature microbiology

Yurkovetskiy L,Guney MH,Kim K,Goh SL,McCauley S,Dauphin A,Diehl WE,Luban J

Sat Dec 01 00:00:00 UTC 2018

Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex.

Nature microbiology

Yurkovetskiy L,Guney MH,Kim K,Goh SL,McCauley S,Dauphin A,Diehl WE,Luban J

Sat Dec 01 00:00:00 UTC 2018

Tri-methylation of ATF7IP by G9a/GLP recruits the chromodomain protein MPP8.

Epigenetics & chromatin

Tsusaka T,Kikuchi M,Shimazu T,Suzuki T,Sohtome Y,Akakabe M,Sodeoka M,Dohmae N,Umehara T,Shinkai Y

Thu Oct 04 00:00:00 UTC 2018

Tri-methylation of ATF7IP by G9a/GLP recruits the chromodomain protein MPP8.

Epigenetics & chromatin

Tsusaka T,Kikuchi M,Shimazu T,Suzuki T,Sohtome Y,Akakabe M,Sodeoka M,Dohmae N,Umehara T,Shinkai Y

Thu Oct 04 00:00:00 UTC 2018

Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2.

Nature genetics

Tchasovnikarova IA,Timms RT,Douse CH,Roberts RC,Dougan G,Kingston RE,Modis Y,Lehner PJ

Sat Jul 01 00:00:00 UTC 2017

Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2.

Nature genetics

Tchasovnikarova IA,Timms RT,Douse CH,Roberts RC,Dougan G,Kingston RE,Modis Y,Lehner PJ

Sat Jul 01 00:00:00 UTC 2017

Characterization of a core region in the A2UCOE that confers effective anti-silencing activity.

Scientific reports

Zhang F,Santilli G,Thrasher AJ

Thu Aug 31 00:00:00 UTC 2017

Characterization of a core region in the A2UCOE that confers effective anti-silencing activity.

Scientific reports

Zhang F,Santilli G,Thrasher AJ

Thu Aug 31 00:00:00 UTC 2017

GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.

Science (New York, N.Y.)

Tchasovnikarova IA,Timms RT,Matheson NJ,Wals K,Antrobus R,Göttgens B,Dougan G,Dawson MA,Lehner PJ

Fri Jun 26 00:00:00 UTC 2015

GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.

Science (New York, N.Y.)

Tchasovnikarova IA,Timms RT,Matheson NJ,Wals K,Antrobus R,Göttgens B,Dougan G,Dawson MA,Lehner PJ

Fri Jun 26 00:00:00 UTC 2015

GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.

Science (New York, N.Y.)

Tchasovnikarova IA,Timms RT,Matheson NJ,Wals K,Antrobus R,Göttgens B,Dougan G,Dawson MA,Lehner PJ

Fri Jun 26 00:00:00 UTC 2015

GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.

Science (New York, N.Y.)

Tchasovnikarova IA,Timms RT,Matheson NJ,Wals K,Antrobus R,Göttgens B,Dougan G,Dawson MA,Lehner PJ

Fri Jun 26 00:00:00 UTC 2015

Physical interaction between MPP8 and PRC1 complex and its implication for regulation of spermatogenesis.