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Bethyl Laboratories
MPRIP/M-RIP Polyclonal Antibody
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MPRIP/M-RIP Antibody (A304-823A) in IP
Detection of human MPRIP/M-RIP by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MPRIP/M-RIP antibody (Product # A304-823A) (lot 1) used for IP at 6 µg per reaction. MPRIP/M-RIP was also immunoprecipitated by rabbit anti-MPRIP/M-RIP antibody A304-822A. For blotting immunoprecipitated MPRIP/M-RIP, A304-823A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minu... View More
Product Details
A304-823A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 975 to 1025 of human Myosin phosphatase Rho-interacting protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat
Target Information
Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: M-RIP; Myosin phosphatase Rho-interacting protein; p116Rip; Rho interacting protein 3; Rho-interacting protein 3; RIP3
Gene Aliases: KIAA0864; M-RIP; MPRIP; MRIP; p116Rip; RHOIP3; RIP3
UniProt ID: (Human) Q6WCQ1
Entrez Gene ID: (Human) 23164
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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