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Invitrogen
MPST Polyclonal Antibody
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FIGURE: 1 / 1
MPST Antibody (PA5-88156) in WB
Western blot analysis of MPST in extracts of various cell lines. Samples were incubated with MPST Polyclonal antibody (Product # PA5-88156) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Product Details
PA5-88156
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-170 of human MPST (NP_0669492)
Conjugate
Form
Liquid
Concentration
2.28 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804698
Product Specific Information
Immunogen sequence: LVSAQWVAEA LRAPRAGQPL QLLDASWYLP KLGRDARREF EERHIPGAAF FDIDQCSDRT SPYDHMLPGA EHFAEYAGRL GVGAATHVVI YDASDQGLYS APRVWWMFRA FGHHAVSLLD GGLRHWLRQN LPLSSGKSQP A; Positive Samples: Mouse liver, Rat liver; Cellular Location: Cell junction, Cytoplasm, Mitochondrion, synapse, synaptosome
Target Information
This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3-mercaptopyruvate sulfurtransferase; MST
Gene Aliases: Mpst; Mst
UniProt ID: (Mouse) Q99J99, (Rat) P97532
Entrez Gene ID: (Mouse) 246221, (Rat) 192172
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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