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MRGPRD (GPCR TGR7) Antibody (AMR-061-200UL) in IHC
Expression of MRGD in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) sections using Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061), (1:200), followed by goat-anti rabbit-AlexaFluor-488. MRGD staining (green) appears in several DRGs (arrows). Cell nuclei are stained with DAPI (blue).
Product Details
AMR-061-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)KSHRLQESLGAVLGR, corresponding to amino acid residues 285-299 of rat MRGPRD, Intracellular, C-terminus
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
May regulate nociceptor function and/or development, including the sensation or modulation of pain. Functions as a specific membrane receptor for beta-alanine. Beta-alanine at micromolar doses specifically evoked Ca(2+) influx in cells expressing the receptor. Beta-alanine decreases forskolin-stimulated cAMP production in cells expressing the receptor, suggesting that the receptor couples with G-protein G(q) and G(i). Localized at the plasma membrane but internalized into the cytoplasm after treatment with beta-alanine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Beta-alanine receptor; G protein-coupled receptor MrgD; G-protein coupled receptor TGR7; Mas-related G protein-coupled receptor d; Mas-related G-protein coupled receptor member D; MAS-related G-protein coupled receptor, member D; MAS-related gene D; MRGD G protein-coupled receptor
Gene Aliases: Gm499; Mgrd; MrgD; Mrgprd; TGR7
UniProt ID: (Rat) Q7TN41, (Mouse) Q91ZB8
Entrez Gene ID: (Rat) 293648, (Mouse) 211578
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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