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MRGPRE Antibody (PA5-102103) in ICC/IF
Immunocytochemistry analysis of MRGPRE in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MRGPRE polyclonal antibody (Product # PA5-102103) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-102103
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MRGPRE(Accession Q86SM8), corresponding to amino acid residues V180-G230.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2851530
Product Specific Information
Antibody detects endogenous levels of total MRGRE.
Target Information
Orphan receptor. May regulate nociceptor function and/or development, including the sensation or modulation of pain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Evolutionary breakpoint transcript 3 protein; G protein-coupled receptor 167; G protein-coupled receptor MrgE; G-protein coupled receptor 167; mas-related G protein-coupled MRGE; Mas-related G protein-coupled receptor F; Mas-related G-protein coupled receptor member E; MAS-related G-protein coupled receptor, member E; MAS-related gene E; MAS-related GPR, member E; MGC138408
Gene Aliases: C130069N09Rik; Ebrt3; GPR167; MGRF; MRGE; MRGPRE
UniProt ID: (Human) Q86SM8, (Mouse) Q91ZB7, (Rat) Q7TN40
Entrez Gene ID: (Human) 116534, (Mouse) 244238, (Rat) 404660
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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