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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • MRPL12 Antibodies

          Proteintech

          MRPL12 Polyclonal Antibody

          31 References
          View all (16) MRPL12 antibodies

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          Datasheet
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          Datasheet
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          Cite MRPL12 Polyclonal Antibody

          • Antibody Testing Data (12)
          MRPL12 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          MRPL12 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 12

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          MRPL12 Antibody (14795-1-AP) in ICC/IF

          Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL12 antibody (Product # 14795-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          MRPL12 Antibody in Immunocytochemistry (ICC/IF)
          MRPL12 Antibody in Immunocytochemistry (ICC/IF)
          MRPL12 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          MRPL12 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          MRPL12 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          MRPL12 Antibody in Western Blot (WB)
          MRPL12 Antibody in Western Blot (WB)
          MRPL12 Antibody in Western Blot (WB)
          MRPL12 Antibody in Western Blot (WB)
          MRPL12 Antibody in Western Blot (WB)
          MRPL12 Antibody in Flow Cytometry (Flow)
          MRPL12 Antibody in Immunoprecipitation (IP)

          Product Details

          14795-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 22 publications 22 publications

          Immunohistochemistry (IHC)

          -
          View 3 publications 3 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          View 3 publications 3 publications

          Flow Cytometry (Flow)

          0.2 µg/1x10^6 cells
          -

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication

          Not applicable (N/A)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          MRPL12 Fusion Protein Ag6504 (1-198 aa encoded by BC002344)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.3 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          The antibody reacts specifically with 21 kDa MRPL12 protein.

          Immunogen sequence: MLPAAARPL WGPCLGLRAA AFRLARRQVP CVCAVRHMRS SGHQRCEALA GAPLDNAPKE YPPKIQQLVQ DIASLTLLEI SDLNELLKKT LKIQDVGLVP MGGVMSGAVP AAAAQEAVEE DIPIAKERTH FTVRLTEAKP VDKVKLIKEI KNYIQGINLV QAKKLVESLP QEIKANVAKA EAEKIKAALE AVGGTVVLE (1-198 aa encoded by BC002344 )

          Target Information

          MRPL12 (mitochondrial ribosomal protein L12), also referred to as 5c5-2, L12mt, MRPL7 or RPML12, is a mammalian mitochondrial ribosomal protein that is involved in protein synthesis within the mitochondrion. MRPL12 is enhanced in growth-stimulated cells as a result of transcriptional activation, suggesting that it may function as a translational regulator of mitochondrial mRNAs. Impairment of MRPL12 leads to reduction in cell growth rate, decreased mitochondrial ATP production and abolition of mitochondrial oxidative phosphorylation. MRPL12 is cleaved during its translocation across the mitochondrial membrane and it exists as dimers that bind the large ribosomal subunit. MRPL12 is 198 amino acids in length, belongs to the ribosomal protein L12P family and is highly expressed in the colon.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: 39S ribosomal protein L12, mitochondrial; 5c5-2; FLJ60124; L12mt; Large ribosomal subunit protein bL12m; MGC8610; MRP-L12; ribosomal protein, mitochondrial, L12

          View more View less

          Gene Aliases: 0610034O11Rik; 1500031N16Rik; 5c5-2; L12mt; MRP-L12; MRP-L31/34; MRPL12; MRPL7; MRPL7/L12; Rpm12; RPML12

          View more View less

          UniProt ID: (Human) P52815, (Mouse) Q9DB15

          View more View less

          Entrez Gene ID: (Human) 6182, (Mouse) 56282, (Rat) 303746

          View more View less

          Function(s)
          RNA binding structural constituent of ribosome protein binding
          Process(es)
          transcription from mitochondrial promoter translation positive regulation of transcription, DNA-templated mitochondrial translational elongation mitochondrial translational termination
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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