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Invitrogen
MRPL12 Polyclonal Antibody
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FIGURE: 1 / 4
MRPL12 Antibody (PA5-30159) in ICC/IF
MRPL12 Polyclonal Antibody detects MRPL12 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min. Green: MRPL12 protein stained by MRPL12 Polyclonal Antibody (Product # PA5-30159) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-30159
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human MRPL12. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.56 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2547633
Product Specific Information
Recommended positive controls: A431, H1299, HeLa, HepG2, mouse brain.
Predicted reactivity: Mouse (84%), Rat (82%), Rhesus Monkey (94%), Bovine (86%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
MRPL12 (mitochondrial ribosomal protein L12), also referred to as 5c5-2, L12mt, MRPL7 or RPML12, is a mammalian mitochondrial ribosomal protein that is involved in protein synthesis within the mitochondrion. MRPL12 is enhanced in growth-stimulated cells as a result of transcriptional activation, suggesting that it may function as a translational regulator of mitochondrial mRNAs. Impairment of MRPL12 leads to reduction in cell growth rate, decreased mitochondrial ATP production and abolition of mitochondrial oxidative phosphorylation. MRPL12 is cleaved during its translocation across the mitochondrial membrane and it exists as dimers that bind the large ribosomal subunit. MRPL12 is 198 amino acids in length, belongs to the ribosomal protein L12P family and is highly expressed in the colon.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 39S ribosomal protein L12, mitochondrial; 5c5-2; FLJ60124; L12mt; Large ribosomal subunit protein bL12m; MGC8610; MRP-L12
Gene Aliases: 0610034O11Rik; 1500031N16Rik; 5c5-2; L12mt; MRP-L12; MRP-L31/34; MRPL12; MRPL7; MRPL7/L12; RPML12
UniProt ID: (Human) P52815, (Mouse) Q9DB15
Entrez Gene ID: (Human) 6182, (Mouse) 56282
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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