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MRPL2 Antibody (PA5-89805) in WB
Western blot analysis of MRPL2 in extracts of various cell lines. Samples were incubated with MRPL2 Polyclonal antibody (Product # PA5-89805) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-89805
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-305 of human MRPL2 (NP_0570342)
Conjugate
Form
Liquid
Concentration
1.54 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805783
Product Specific Information
Immunogen sequence: MALCALTRAL RSLNLAPPTV AAPAPSLFPA AQMMNNGLLQ QPSALMLLPC RPVLTSVALN ANFVSWKSRT KYTITPVKMR KSGGRDHTGR IRVHGIGGGH KQRYRMIDFL RFRPEETKSG PFEEKVIQVR YDPCRSADIA LVAGGSRKRW IIATENMQAG DTILNSNHIG RMAVAAREGD AHPLGALPVG TLINNVESEP GRGAQYIRAA GTCGVLLRKV NGTAIIQLPS KRQMQVLETC VATVGRVSNV DHNKRVIGKA GRNRWLGKRP NSGRWHRKGG WAGRKIRPLP PMKSYVKLPS ASAQS; Positive Samples: Mouse kidney, Mouse lung; Cellular Location: Mitochondrion
Target Information
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 12q. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 39S ribosomal protein L2, mitochondrial; L2mt; Large ribosomal subunit protein uL2m; MRP-L2; ribosomal protein, mitochondrial, L14
Gene Aliases: CGI-22; MRP-L14; MRPL2; RPML14
UniProt ID: (Human) Q5T653, (Mouse) Q9D773
Entrez Gene ID: (Human) 51069, (Mouse) 27398
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