Proteintech

MRPS16 Polyclonal Antibody

MRPS16 Antibody in Immunocytochemistry (ICC/IF)

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MRPS16 Antibody (16735-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA ) fixed HepG2 cells using 16735-1-AP (MRPS16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MRPS16 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16735-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 16 publications 16 publications

Immunocytochemistry (ICC/IF)

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MRPS16 Fusion Protein Ag9749 (1-137 aa encoded by BC021106)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.14 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MVHLTTLLC KAYRGGHLTI RLALGGCTNR PFYRIVAAHN KCPRDGRFVE QLGSYDPLPN SHGEKLVALN LDRIRHWIGC GAHLSKPMEK LLGLAGFFPL HPMMITNAER LRRKRAREVL LASQKTDAEA TDTEATET (1-137 aa encoded by BC021106)

Target Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S16P family. The encoded protein is one of the most highly conserved ribosomal proteins between mammalian and yeast mitochondria. Three pseudogenes (located at 8q21. 3, 20q13. 32, 22q12-q13. 1) for this gene have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease.

Human molecular genetics

Amarasekera SSC,Hock DH,Lake NJ,Calvo SE,Grønborg SW,Krzesinski EI,Amor DJ,Fahey MC,Simons C,Wibrand F,Mootha VK,Lek M,Lunke S,Stark Z,Østergaard E,Christodoulou J,Thorburn DR,Stroud DA,Compton AG

Thu Jul 20 00:00:00 UTC 2023

Tetracycline-dependent inhibition of mitoribosome protein elongation in mitochondrial disease mutant cells suppresses IRE1α to promote cell survival.

bioRxiv : the preprint server for biology

Ronayne CT,Bennett CF,Perry EA,Kantorovich N,Puigserver P

Tue Mar 21 00:00:00 UTC 2023

Non-canonical phosphoglycerate dehydrogenase activity promotes liver cancer growth via mitochondrial translation and respiratory metabolism.

The EMBO journal

Shu Y,Hao Y,Feng J,Liu H,Li ST,Feng J,Jiang Z,Ye L,Zhou Y,Sun Y,Zhou Z,Wei H,Gao P,Zhang H,Sun L

Thu Dec 01 00:00:00 UTC 2022

Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan.

Aging cell

Richman TR,Ermer JA,Siira SJ,Kuznetsova I,Brosnan CA,Rossetti G,Baker J,Perks KL,Cserne Szappanos H,Viola HM,Gray N,Larance M,Hool LC,Zuryn S,Rackham O,Filipovska A

Thu Jul 01 00:00:00 UTC 2021

Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors.

eLife

Dennerlein S,Poerschke S,Oeljeklaus S,Wang C,Richter-Dennerlein R,Sattmann J,Bauermeister D,Hanitsch E,Stoldt S,Langer T,Jakobs S,Warscheid B,Rehling P

Fri Dec 31 00:00:00 UTC 2021

Cardiolipin is required for membrane docking of mitochondrial ribosomes and protein synthesis.

Journal of cell science

Lee RG,Gao J,Siira SJ,Shearwood AM,Ermer JA,Hofferek V,Mathews JC,Zheng M,Reid GE,Rackham O,Filipovska A

Fri Jul 24 00:00:00 UTC 2020

Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.

The EMBO journal

Ferreira N,Perks KL,Rossetti G,Rudler DL,Hughes LA,Ermer JA,Scott LH,Kuznetsova I,Richman TR,Narayana VK,Abudulai LN,Shearwood AJ,Cserne Szappanos H,Tull D,Yeoh GC,Hool LC,Filipovska A,Rackham O

Mon Dec 16 00:00:00 UTC 2019

MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition.

Cell reports

Busch JD,Cipullo M,Atanassov I,Bratic A,Silva Ramos E,Schöndorf T,Li X,Pearce SF,Milenkovic D,Rorbach J,Larsson NG

Tue Nov 05 00:00:00 UTC 2019

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

Science advances

Rudler DL,Hughes LA,Perks KL,Richman TR,Kuznetsova I,Ermer JA,Abudulai LN,Shearwood AJ,Viola HM,Hool LC,Siira SJ,Rackham O,Filipovska A

Sun Dec 01 00:00:00 UTC 2019

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Nucleic acids research

Lavdovskaia E,Kolander E,Steube E,Mai MM,Urlaub H,Richter-Dennerlein R

Wed Sep 19 00:00:00 UTC 2018

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Nucleic acids research

Lavdovskaia E,Kolander E,Steube E,Mai MM,Urlaub H,Richter-Dennerlein R

Wed Sep 19 00:00:00 UTC 2018

Concerted regulation of mitochondrial and nuclear non-coding RNAs by a dual-targeted RNase Z.

EMBO reports

Siira SJ,Rossetti G,Richman TR,Perks K,Ermer JA,Kuznetsova I,Hughes L,Shearwood AJ,Viola HM,Hool LC,Rackham O,Filipovska A

Mon Oct 01 00:00:00 UTC 2018

Concerted regulation of mitochondrial and nuclear non-coding RNAs by a dual-targeted RNase Z.

EMBO reports

Siira SJ,Rossetti G,Richman TR,Perks K,Ermer JA,Kuznetsova I,Hughes L,Shearwood AJ,Viola HM,Hool LC,Rackham O,Filipovska A

Mon Oct 01 00:00:00 UTC 2018

Biallelic Mutations in MRPS34 Lead to Instability of the Small Mitoribosomal Subunit and Leigh Syndrome.

American journal of human genetics

Lake NJ,Webb BD,Stroud DA,Richman TR,Ruzzenente B,Compton AG,Mountford HS,Pulman J,Zangarelli C,Rio M,Boddaert N,Assouline Z,Sherpa MD,Schadt EE,Houten SM,Byrnes J,McCormick EM,Zolkipli-Cunningham Z,Haude K,Zhang Z,Retterer K,Bai R,Calvo SE,Mootha VK,Christodoulou J,Rötig A,Filipovska A,Cristian I,Falk MJ,Metodiev MD,Thorburn DR

Thu Aug 03 00:00:00 UTC 2017

Biallelic Mutations in MRPS34 Lead to Instability of the Small Mitoribosomal Subunit and Leigh Syndrome.

American journal of human genetics

Thu Aug 03 00:00:00 UTC 2017

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Science advances

Perks KL,Ferreira N,Richman TR,Ermer JA,Kuznetsova I,Shearwood AJ,Lee RG,Viola HM,Johnstone VPA,Matthews V,Hool LC,Rackham O,Filipovska A

Tue Aug 01 00:00:00 UTC 2017

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Science advances

Perks KL,Ferreira N,Richman TR,Ermer JA,Kuznetsova I,Shearwood AJ,Lee RG,Viola HM,Johnstone VPA,Matthews V,Hool LC,Rackham O,Filipovska A

Tue Aug 01 00:00:00 UTC 2017

Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly.

Cell reports

Rackham O,Busch JD,Matic S,Siira SJ,Kuznetsova I,Atanassov I,Ermer JA,Shearwood AM,Richman TR,Stewart JB,Mourier A,Milenkovic D,Larsson NG,Filipovska A

Tue Aug 16 00:00:00 UTC 2016

Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly.

Cell reports

Rackham O,Busch JD,Matic S,Siira SJ,Kuznetsova I,Atanassov I,Ermer JA,Shearwood AM,Richman TR,Stewart JB,Mourier A,Milenkovic D,Larsson NG,Filipovska A

Tue Aug 16 00:00:00 UTC 2016

Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.

Nature communications

Richman TR,Spåhr H,Ermer JA,Davies SM,Viola HM,Bates KA,Papadimitriou J,Hool LC,Rodger J,Larsson NG,Rackham O,Filipovska A

Mon Jun 20 00:00:00 UTC 2016

Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.

Nature communications

Richman TR,Spåhr H,Ermer JA,Davies SM,Viola HM,Bates KA,Papadimitriou J,Hool LC,Rodger J,Larsson NG,Rackham O,Filipovska A

Mon Jun 20 00:00:00 UTC 2016

Mutation in MRPS34 compromises protein synthesis and causes mitochondrial dysfunction.

PLoS genetics

Richman TR,Ermer JA,Davies SM,Perks KL,Viola HM,Shearwood AM,Hool LC,Rackham O,Filipovska A

Sun Mar 01 00:00:00 UTC 2015

Mutation in MRPS34 compromises protein synthesis and causes mitochondrial dysfunction.

PLoS genetics

Richman TR,Ermer JA,Davies SM,Perks KL,Viola HM,Shearwood AM,Hool LC,Rackham O,Filipovska A

Sun Mar 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: 28S ribosomal protein S16, mitochondrial; MRP-S16; S16mt; Small ribosomal subunit protein bS16m

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Gene Aliases: 1500011E11Rik; CGI-132; COXPD2; Mprs16; MRP-S16; MRPS16; RPMS16

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UniProt ID: (Human) Q9Y3D3, (Mouse) Q9CPX7

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Entrez Gene ID: (Human) 51021, (Rat) 688912, (Mouse) 66242

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Function(s)

structural constituent of ribosome protein binding molecular_function

Process(es)

mitochondrial translation translation mitochondrial translational elongation mitochondrial translational termination biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MRPS16 Polyclonal Antibody

MRPS16 Antibody (16735-1-AP) in ICC/IF

Product Details

16735-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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