Proteintech

MRPS18B Polyclonal Antibody

MRPS18B Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

MRPS18B Antibody (16139-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 andand Rhodamine-labeled goat anti-rabbit IGG (red).

MRPS18B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MRPS18B Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16139-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 45 publications 45 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MRPS18B Fusion Protein Ag9198 (1-258 aa encoded by BC005373)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.38 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognizes specifically the 29 kDa MRPS18B protein.

Immunogen sequence: MAASVLNTV LRRLPMLSLF RGSHRVQVPL QTLCTKAPSE EDSLSSVPIS PYKDEPWKYL ESEEYQERYG SRPVWADYRR NHKGGVPPQR TRKTCIRRNK VVGNPCPICR DHKLHVDFRN VKLLEQFVCA HTGIIFYAPY TGVCVKQHKR LTQAIQKARD HGLLIYHIPQ VEPRDLDFST SHGAVSATPP APTLVSSDPW YPWYNWKQPP ERELSRLRRL YQGHLQEESG PPPESMPKMP PRTPAEASST GQTGPQSAL (1-258 aa encoded by BC005373 )

Target Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Human Mitoribosome Profiling: A Re-engineered Approach Tailored to Study Mitochondrial Translation.

Methods in molecular biology (Clifton, N.J.)

Soto I,Couvillion M,Stirling Churchman L

Mon May 15 00:00:00 UTC 2023

A kinetic dichotomy between mitochondrial and nuclear gene expression drives OXPHOS biogenesis.

bioRxiv : the preprint server for biology

McShane E,Couvillion M,Ietswaart R,Prakash G,Smalec BM,Soto I,Baxter-Koenigs AR,Choquet K,Churchman LS

Wed Sep 06 00:00:00 UTC 2023

Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes.

Genome biology

Soto I,Couvillion M,Hansen KG,McShane E,Moran JC,Barrientos A,Churchman LS

Tue Aug 09 00:00:00 UTC 2022

Ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) is a novel complex III-specific assembly factor in mitochondria.

Molecular biology of the cell

Chojnacka KJ,Elancheliyan P,Mussulini BHM,Mohanraj K,Callegari S,Gosk A,Banach T,Góral T,Szczepanowska K,Rehling P,Serwa RA,Chacinska A

Fri Apr 01 00:00:00 UTC 2022

Mitochondrial microproteins link metabolic cues to respiratory chain biogenesis.

Cell reports

Liang C,Zhang S,Robinson D,Ploeg MV,Wilson R,Nah J,Taylor D,Beh S,Lim R,Sun L,Muoio DM,Stroud DA,Ho L

Tue Aug 16 00:00:00 UTC 2022

A deafness-associated tRNA mutation caused pleiotropic effects on the m1G37 modification, processing, stability and aminoacylation of tRNAIle and mitochondrial translation.

Nucleic acids research

Meng F,Zhou M,Xiao Y,Mao X,Zheng J,Lin J,Lin T,Ye Z,Cang X,Fu Y,Wang M,Guan MX

Mon Jan 25 00:00:00 UTC 2021

Messenger RNA delivery to mitoribosomes - hints from a bacterial toxin.

The FEBS journal

Bruni F,Proctor-Kent Y,Lightowlers RN,Chrzanowska-Lightowlers ZM

Fri Jan 01 00:00:00 UTC 2021

Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors.

eLife

Dennerlein S,Poerschke S,Oeljeklaus S,Wang C,Richter-Dennerlein R,Sattmann J,Bauermeister D,Hanitsch E,Stoldt S,Langer T,Jakobs S,Warscheid B,Rehling P

Fri Dec 31 00:00:00 UTC 2021

YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.

European journal of immunology

Nandi S,Liang G,Sindhava V,Angireddy R,Basu A,Banerjee S,Hodawadekar S,Zhang Y,Avadhani NG,Sen R,Atchison ML

Mon Jun 01 00:00:00 UTC 2020

Inducible Germline IgMs Bridge Trypanosome Lytic Factor Assembly and Parasite Recognition.

Cell host & microbe

Verdi J,Zipkin R,Hillman E,Gertsch RA,Pangburn SJ,Thomson R,Papavasiliou N,Sternberg J,Raper J

Wed Jul 08 00:00:00 UTC 2020

Inducible Germline IgMs Bridge Trypanosome Lytic Factor Assembly and Parasite Recognition.

Cell host & microbe

Verdi J,Zipkin R,Hillman E,Gertsch RA,Pangburn SJ,Thomson R,Papavasiliou N,Sternberg J,Raper J

Wed Jul 08 00:00:00 UTC 2020

MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex.

EMBO reports

Wang C,Richter-Dennerlein R,Pacheu-Grau D,Liu F,Zhu Y,Dennerlein S,Rehling P

Tue Jan 07 00:00:00 UTC 2020

RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.

PLoS genetics

Reyes A,Favia P,Vidoni S,Petruzzella V,Zeviani M

Wed Jul 01 00:00:00 UTC 2020

FMRP Control of Ribosome Translocation Promotes Chromatin Modifications and Alternative Splicing of Neuronal Genes Linked to Autism.

Cell reports

Shah S,Molinaro G,Liu B,Wang R,Huber KM,Richter JD

Tue Mar 31 00:00:00 UTC 2020

Expression pattern of MRPS18 family genes in malignantly transformed b-cells.

Experimental oncology

Kovalevska L,Kashuba E

Tue Dec 01 00:00:00 UTC 2020

TRMT2B is responsible for both tRNA and rRNA m⁵U-methylation in human mitochondria.

RNA biology

Powell CA,Minczuk M

Wed Apr 01 00:00:00 UTC 2020

METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis.

Nucleic acids research

Van Haute L,Hendrick AG,D'Souza AR,Powell CA,Rebelo-Guiomar P,Harbour ME,Ding S,Fearnley IM,Andrews B,Minczuk M

Mon Nov 04 00:00:00 UTC 2019

Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.

Human molecular genetics

Pulman J,Ruzzenente B,Bianchi L,Rio M,Boddaert N,Munnich A,Rötig A,Metodiev MD

Wed May 01 00:00:00 UTC 2019

Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.

Human molecular genetics

Pulman J,Ruzzenente B,Bianchi L,Rio M,Boddaert N,Munnich A,Rötig A,Metodiev MD

Wed May 01 00:00:00 UTC 2019

A variant in MRPS14 (uS14m) causes perinatal hypertrophic cardiomyopathy with neonatal lactic acidosis, growth retardation, dysmorphic features and neurological involvement.

Human molecular genetics

Jackson CB,Huemer M,Bolognini R,Martin F,Szinnai G,Donner BC,Richter U,Battersby BJ,Nuoffer JM,Suomalainen A,Schaller A

Fri Feb 15 00:00:00 UTC 2019

A variant in MRPS14 (uS14m) causes perinatal hypertrophic cardiomyopathy with neonatal lactic acidosis, growth retardation, dysmorphic features and neurological involvement.

Human molecular genetics

Jackson CB,Huemer M,Bolognini R,Martin F,Szinnai G,Donner BC,Richter U,Battersby BJ,Nuoffer JM,Suomalainen A,Schaller A

Fri Feb 15 00:00:00 UTC 2019

Mitochondrial stress response triggered by defects in protein synthesis quality control.

Life science alliance

Richter U,Ng KY,Suomi F,Marttinen P,Turunen T,Jackson C,Suomalainen A,Vihinen H,Jokitalo E,Nyman TA,Isokallio MA,Stewart JB,Mancini C,Brusco A,Seneca S,Lombès A,Taylor RW,Battersby BJ

Fri Feb 01 00:00:00 UTC 2019

RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA.

Frontiers in genetics

Reyes A,Rusecka J,Tońska K,Zeviani M

Thu Feb 09 00:00:00 UTC 2023

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Nucleic acids research

Lavdovskaia E,Kolander E,Steube E,Mai MM,Urlaub H,Richter-Dennerlein R

Wed Sep 19 00:00:00 UTC 2018

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Nucleic acids research

Lavdovskaia E,Kolander E,Steube E,Mai MM,Urlaub H,Richter-Dennerlein R

Wed Sep 19 00:00:00 UTC 2018

Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis.

Human mutation

Ruzzenente B,Assouline Z,Barcia G,Rio M,Boddaert N,Munnich A,Rötig A,Metodiev MD

Sat Dec 01 00:00:00 UTC 2018

Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis.

Human mutation

Ruzzenente B,Assouline Z,Barcia G,Rio M,Boddaert N,Munnich A,Rötig A,Metodiev MD

Sat Dec 01 00:00:00 UTC 2018

LONP1 Is Required for Maturation of a Subset of Mitochondrial Proteins, and Its Loss Elicits an Integrated Stress Response.

Molecular and cellular biology

Zurita Rendón O,Shoubridge EA

Mon Oct 15 00:00:00 UTC 2018

The MRPS18-2 protein levels correlate with prostate tumor progression and it induces CXCR4-dependent migration of cancer cells.

Scientific reports