Proteintech

MRPS25 Polyclonal Antibody

FIGURE: 1 / 5

MRPS25 Antibody (15277-1-AP) in WB

HEK-293 cells were subjected to SDS PAGE followed by western blot with 15277-1-AP (MRPS25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

MRPS25 Antibody in Immunoprecipitation (IP)

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Product Details

15277-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MRPS25 Fusion Protein Ag7395 (1-173 aa encoded by BC003590)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody specifically recognizes the 18 kDa MRPS25 protein.

Immunogen sequence: MPMKGRFPI RRTLQYLSQG NVVFKDSVKV MTVNYNTHGE LGEGARKFVF FNIPQIQYKN PWVQIMMFKN MTPSPFLRFY LDSGEQVLVD VETKSNKEIM EHIRKILGKN EETLREEEEE KKQLSHPANF GPRKYCLREC ICEVEGQVPC PSLVPLPKEM RGKYKAALKA DAQD (1-173 aa encoded by BC003590)

Target Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice.

Methods in molecular biology (Clifton, N.J.)

Ruzzenente B,Metodiev MD

Thu Apr 01 00:00:00 UTC 2021

Mutation in MRPS34 compromises protein synthesis and causes mitochondrial dysfunction.

PLoS genetics

Richman TR,Ermer JA,Davies SM,Perks KL,Viola HM,Shearwood AM,Hool LC,Rackham O,Filipovska A

Sun Mar 01 00:00:00 UTC 2015

Mutation in MRPS34 compromises protein synthesis and causes mitochondrial dysfunction.

PLoS genetics

Richman TR,Ermer JA,Davies SM,Perks KL,Viola HM,Shearwood AM,Hool LC,Rackham O,Filipovska A

Sun Mar 01 00:00:00 UTC 2015

Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency.

Biochimica et biophysica acta

Serre V,Rozanska A,Beinat M,Chretien D,Boddaert N,Munnich A,Rötig A,Chrzanowska-Lightowlers ZM

Thu Aug 01 00:00:00 UTC 2013

Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency.

Biochimica et biophysica acta

Serre V,Rozanska A,Beinat M,Chretien D,Boddaert N,Munnich A,Rötig A,Chrzanowska-Lightowlers ZM

Thu Aug 01 00:00:00 UTC 2013

Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit.

The Biochemical journal

Dennerlein S,Rozanska A,Wydro M,Chrzanowska-Lightowlers ZM,Lightowlers RN

Wed Sep 15 00:00:00 UTC 2010

Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit.

The Biochemical journal

Dennerlein S,Rozanska A,Wydro M,Chrzanowska-Lightowlers ZM,Lightowlers RN

Wed Sep 15 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: 28S ribosomal protein S25, mitochondrial; mitochondrial 28S ribosomal protein S25; MRP-S25; rt25; S25mt; Small ribosomal subunit protein mS25

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Gene Aliases: 2810429N01Rik; MRP-S25; MRPS25; RPMS25; Shd10

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UniProt ID: (Human) P82663, (Mouse) Q9D125, (Rat) Q4QR80

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Entrez Gene ID: (Human) 64432, (Mouse) 64658, (Rat) 297459

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Function(s)

structural constituent of ribosome

Process(es)

mitochondrial translation mitochondrial translational elongation mitochondrial translational termination

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MRPS25 Polyclonal Antibody

MRPS25 Antibody (15277-1-AP) in WB

Product Details

15277-1-AP

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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