Proteintech

MRPS35 Polyclonal Antibody

MRPS35 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

MRPS35 Antibody (16457-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MRPS35 antibody (Product # 16457-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

MRPS35 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MRPS35 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16457-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 25 publications 25 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MRPS35 Fusion Protein Ag9430 (1-323 aa encoded by BC015862)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against the full length MRPS35 protein. It specifically reacts with the 37 kDa MRPS35protein.

Immunogen sequence: MAAAALPAW LSLQSRARTL RAFSTAVYSA TPVPTPSLPE RTPGNERPPR RKALPPRTEK MAVDQDWPSV YPVAAPFKPS AVPLPVRMGY PVKKGVPMAK EGNLELLKIP NFLHLTPVAI KKHCEALKDF CTEWPAALDS DEKCEKHFPI EIDSTDYVSS GPSVRNPRAR VVVLRVKLSS LNLDDHAKKK LIKLVGERYC KTTDVLTIKT DRCPLRRQNY DYAVYLLTVL YHESWNTEEW EKSKTEADME EYIWENSSSE RNILETLLQM KAAEKNMEIN KEELLGTKEI EEYKKSVVSL KNEEENENSI SQYKESVKRL LNVT (1-323 aa encoded by BC015862)

Target Information

Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for protein synthesis within the mitochondrion. The mitoribosomes are composed of a 4:1 ratio of protein to RNA, with the proteins forming two subunits, the 28S subunit and the 39S subunit. Across species, the proteins that make up the mitoribosome subunits vary greatly in sequence, preventing easy recognition by sequence homology. MRP-S35 (mitochondrial ribosomal protein S35), also known as MDS023, MRPS28 or HDCMD11P, is a 323 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 28S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. Existing as two alternatively spliced isoforms, MRP-S35 is encoded by a gene located on human chromosome 10, which houses over 1,200 genes and comprises nearly 4. 5% of the human genome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease.

Human molecular genetics

Amarasekera SSC,Hock DH,Lake NJ,Calvo SE,Grønborg SW,Krzesinski EI,Amor DJ,Fahey MC,Simons C,Wibrand F,Mootha VK,Lek M,Lunke S,Stark Z,Østergaard E,Christodoulou J,Thorburn DR,Stroud DA,Compton AG

Thu Jul 20 00:00:00 UTC 2023

Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals.

Cell metabolism

Kenny TC,Khan A,Son Y,Yue L,Heissel S,Sharma A,Pasolli HA,Liu Y,Gamazon ER,Alwaseem H,Hite RK,Birsoy K

Tue Jun 06 00:00:00 UTC 2023

Sucrose Gradient Analysis of Human Mitochondrial Ribosomes and RNA.

Methods in molecular biology (Clifton, N.J.)

Ng KY,Battersby BJ

Mon May 15 00:00:00 UTC 2023

circMRPS35 promotes malignant progression and cisplatin resistance in hepatocellular carcinoma.

Molecular therapy : the journal of the American Society of Gene Therapy

Li P,Song R,Yin F,Liu M,Liu H,Ma S,Jia X,Lu X,Zhong Y,Yu L,Li X,Li X

Wed Jan 05 00:00:00 UTC 2022

circMRPS35 promotes malignant progression and cisplatin resistance in hepatocellular carcinoma.

Molecular therapy : the journal of the American Society of Gene Therapy

Li P,Song R,Yin F,Liu M,Liu H,Ma S,Jia X,Lu X,Zhong Y,Yu L,Li X,Li X

Wed Jan 05 00:00:00 UTC 2022

Nonstop mRNAs generate a ground state of mitochondrial gene expression noise.

Science advances

Ng KY,Lutfullahoglu Bal G,Richter U,Safronov O,Paulin L,Dunn CD,Paavilainen VO,Richer J,Newman WG,Taylor RW,Battersby BJ

Wed Nov 16 00:00:00 UTC 2022

Dysfunctional mitochondrial translation and combined oxidative phosphorylation deficiency in a mouse model of hepatoencephalopathy due to Gfm1 mutations.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Molina-Berenguer M,Vila-Julià F,Pérez-Ramos S,Salcedo-Allende MT,Cámara Y,Torres-Torronteras J,Martí R

Sat Jan 01 00:00:00 UTC 2022

Non-canonical phosphoglycerate dehydrogenase activity promotes liver cancer growth via mitochondrial translation and respiratory metabolism.

The EMBO journal

Shu Y,Hao Y,Feng J,Liu H,Li ST,Feng J,Jiang Z,Ye L,Zhou Y,Sun Y,Zhou Z,Wei H,Gao P,Zhang H,Sun L

Thu Dec 01 00:00:00 UTC 2022

Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice.

Methods in molecular biology (Clifton, N.J.)

Ruzzenente B,Metodiev MD

Thu Apr 01 00:00:00 UTC 2021

Cardiolipin is required for membrane docking of mitochondrial ribosomes and protein synthesis.

Journal of cell science

Lee RG,Gao J,Siira SJ,Shearwood AM,Ermer JA,Hofferek V,Mathews JC,Zheng M,Reid GE,Rackham O,Filipovska A

Fri Jul 24 00:00:00 UTC 2020

TRMT2B is responsible for both tRNA and rRNA m⁵U-methylation in human mitochondria.

RNA biology

Powell CA,Minczuk M

Wed Apr 01 00:00:00 UTC 2020

Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.

The EMBO journal

Ferreira N,Perks KL,Rossetti G,Rudler DL,Hughes LA,Ermer JA,Scott LH,Kuznetsova I,Richman TR,Narayana VK,Abudulai LN,Shearwood AJ,Cserne Szappanos H,Tull D,Yeoh GC,Hool LC,Filipovska A,Rackham O

Mon Dec 16 00:00:00 UTC 2019

MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition.

Cell reports

Busch JD,Cipullo M,Atanassov I,Bratic A,Silva Ramos E,Schöndorf T,Li X,Pearce SF,Milenkovic D,Rorbach J,Larsson NG

Tue Nov 05 00:00:00 UTC 2019

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

Science advances

Rudler DL,Hughes LA,Perks KL,Richman TR,Kuznetsova I,Ermer JA,Abudulai LN,Shearwood AJ,Viola HM,Hool LC,Siira SJ,Rackham O,Filipovska A

Sun Dec 01 00:00:00 UTC 2019

RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA.

Frontiers in genetics

Reyes A,Rusecka J,Tońska K,Zeviani M

Thu Feb 09 00:00:00 UTC 2023

Concerted regulation of mitochondrial and nuclear non-coding RNAs by a dual-targeted RNase Z.

EMBO reports

Siira SJ,Rossetti G,Richman TR,Perks K,Ermer JA,Kuznetsova I,Hughes L,Shearwood AJ,Viola HM,Hool LC,Rackham O,Filipovska A

Mon Oct 01 00:00:00 UTC 2018

Concerted regulation of mitochondrial and nuclear non-coding RNAs by a dual-targeted RNase Z.

EMBO reports

Siira SJ,Rossetti G,Richman TR,Perks K,Ermer JA,Kuznetsova I,Hughes L,Shearwood AJ,Viola HM,Hool LC,Rackham O,Filipovska A

Mon Oct 01 00:00:00 UTC 2018

Biallelic Mutations in MRPS34 Lead to Instability of the Small Mitoribosomal Subunit and Leigh Syndrome.

American journal of human genetics

Lake NJ,Webb BD,Stroud DA,Richman TR,Ruzzenente B,Compton AG,Mountford HS,Pulman J,Zangarelli C,Rio M,Boddaert N,Assouline Z,Sherpa MD,Schadt EE,Houten SM,Byrnes J,McCormick EM,Zolkipli-Cunningham Z,Haude K,Zhang Z,Retterer K,Bai R,Calvo SE,Mootha VK,Christodoulou J,Rötig A,Filipovska A,Cristian I,Falk MJ,Metodiev MD,Thorburn DR

Thu Aug 03 00:00:00 UTC 2017

Biallelic Mutations in MRPS34 Lead to Instability of the Small Mitoribosomal Subunit and Leigh Syndrome.

American journal of human genetics

Thu Aug 03 00:00:00 UTC 2017

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Science advances

Perks KL,Ferreira N,Richman TR,Ermer JA,Kuznetsova I,Shearwood AJ,Lee RG,Viola HM,Johnstone VPA,Matthews V,Hool LC,Rackham O,Filipovska A

Tue Aug 01 00:00:00 UTC 2017

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Science advances

Perks KL,Ferreira N,Richman TR,Ermer JA,Kuznetsova I,Shearwood AJ,Lee RG,Viola HM,Johnstone VPA,Matthews V,Hool LC,Rackham O,Filipovska A

Tue Aug 01 00:00:00 UTC 2017

Transcriptomic and proteomic landscape of mitochondrial dysfunction reveals secondary coenzyme Q deficiency in mammals.

eLife

Kühl I,Miranda M,Atanassov I,Kuznetsova I,Hinze Y,Mourier A,Filipovska A,Larsson NG

Tue Nov 14 00:00:00 UTC 2017

Transcriptomic and proteomic landscape of mitochondrial dysfunction reveals secondary coenzyme Q deficiency in mammals.

eLife

Kühl I,Miranda M,Atanassov I,Kuznetsova I,Hinze Y,Mourier A,Filipovska A,Larsson NG

Tue Nov 14 00:00:00 UTC 2017

CLPP coordinates mitoribosomal assembly through the regulation of ERAL1 levels.

The EMBO journal

Szczepanowska K,Maiti P,Kukat A,Hofsetz E,Nolte H,Senft K,Becker C,Ruzzenente B,Hornig-Do HT,Wibom R,Wiesner RJ,Krüger M,Trifunovic A

Thu Dec 01 00:00:00 UTC 2016

CLPP coordinates mitoribosomal assembly through the regulation of ERAL1 levels.

The EMBO journal

Szczepanowska K,Maiti P,Kukat A,Hofsetz E,Nolte H,Senft K,Becker C,Ruzzenente B,Hornig-Do HT,Wibom R,Wiesner RJ,Krüger M,Trifunovic A

Thu Dec 01 00:00:00 UTC 2016

Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly.

Cell reports

Rackham O,Busch JD,Matic S,Siira SJ,Kuznetsova I,Atanassov I,Ermer JA,Shearwood AM,Richman TR,Stewart JB,Mourier A,Milenkovic D,Larsson NG,Filipovska A

Tue Aug 16 00:00:00 UTC 2016

Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly.

Cell reports

Rackham O,Busch JD,Matic S,Siira SJ,Kuznetsova I,Atanassov I,Ermer JA,Shearwood AM,Richman TR,Stewart JB,Mourier A,Milenkovic D,Larsson NG,Filipovska A

Tue Aug 16 00:00:00 UTC 2016

Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.

Nature communications

Richman TR,Spåhr H,Ermer JA,Davies SM,Viola HM,Bates KA,Papadimitriou J,Hool LC,Rodger J,Larsson NG,Rackham O,Filipovska A

Mon Jun 20 00:00:00 UTC 2016

Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.

Nature communications

Richman TR,Spåhr H,Ermer JA,Davies SM,Viola HM,Bates KA,Papadimitriou J,Hool LC,Rodger J,Larsson NG,Rackham O,Filipovska A

Mon Jun 20 00:00:00 UTC 2016

Mutation in MRPS34 compromises protein synthesis and causes mitochondrial dysfunction.

PLoS genetics

Richman TR,Ermer JA,Davies SM,Perks KL,Viola HM,Shearwood AM,Hool LC,Rackham O,Filipovska A

Sun Mar 01 00:00:00 UTC 2015

Mutation in MRPS34 compromises protein synthesis and causes mitochondrial dysfunction.

PLoS genetics

Richman TR,Ermer JA,Davies SM,Perks KL,Viola HM,Shearwood AM,Hool LC,Rackham O,Filipovska A

Sun Mar 01 00:00:00 UTC 2015

SLIRP Regulates the Rate of Mitochondrial Protein Synthesis and Protects LRPPRC from Degradation.

PLoS genetics

Lagouge M,Mourier A,Lee HJ,Spåhr H,Wai T,Kukat C,Silva Ramos E,Motori E,Busch JD,Siira S,Kremmer E,Filipovska A,Larsson NG

Sat Aug 01 00:00:00 UTC 2015

SLIRP Regulates the Rate of Mitochondrial Protein Synthesis and Protects LRPPRC from Degradation.

PLoS genetics

Lagouge M,Mourier A,Lee HJ,Spåhr H,Wai T,Kukat C,Silva Ramos E,Motori E,Busch JD,Siira S,Kremmer E,Filipovska A,Larsson NG

Sat Aug 01 00:00:00 UTC 2015

Tissue-specific loss of DARS2 activates stress responses independently of respiratory chain deficiency in the heart.

Cell metabolism

Dogan SA,Pujol C,Maiti P,Kukat A,Wang S,Hermans S,Senft K,Wibom R,Rugarli EI,Trifunovic A

Tue Mar 04 00:00:00 UTC 2014

AFG3L2 supports mitochondrial protein synthesis and Purkinje cell survival.

The Journal of clinical investigation

Almajan ER,Richter R,Paeger L,Martinelli P,Barth E,Decker T,Larsson NG,Kloppenburg P,Langer T,Rugarli EI

Thu Nov 01 00:00:00 UTC 2012

AFG3L2 supports mitochondrial protein synthesis and Purkinje cell survival.

The Journal of clinical investigation

Almajan ER,Richter R,Paeger L,Martinelli P,Barth E,Decker T,Larsson NG,Kloppenburg P,Langer T,Rugarli EI

Thu Nov 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: 28S ribosomal protein S28, mitochondrial; 28S ribosomal protein S35, mitochondrial; mitochondrial ribosomal protein S28; mitochondrial ribosomal protein S34; MRP-S28; MRP-S35; MRPS35; S28mt; S35mt; Small ribosomal subunit protein mS35

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Gene Aliases: HDCMD11P; MDS023; MDSO23; MRP-S28; MRPS28; MRPS35; PSEC0213

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UniProt ID: (Human) P82673, (Mouse) Q8BJZ4

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Entrez Gene ID: (Human) 60488, (Rat) 297727, (Mouse) 232536

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Function(s)

structural constituent of ribosome poly(A) RNA binding

Process(es)

DNA damage response, detection of DNA damage mitochondrial translational elongation mitochondrial translational termination biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MRPS35 Polyclonal Antibody

MRPS35 Antibody (16457-1-AP) in ICC/IF

Product Details

16457-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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