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Invitrogen
MRPS35 Polyclonal Antibody
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FIGURE: 1 / 2
MRPS35 Antibody (PA5-101349) in ICC/IF
Immunocytochemistry analysis of MRPS35 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MRPS35 polyclonal antibody (Product # PA5-101349) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-101349
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MRPS35(Accession P82673), corresponding to amino acid residues T18-S68.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2850785
Product Specific Information
Antibody detects endogenous levels of total MRPS35.
Target Information
Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for protein synthesis within the mitochondrion. The mitoribosomes are composed of a 4:1 ratio of protein to RNA, with the proteins forming two subunits, the 28S subunit and the 39S subunit. Across species, the proteins that make up the mitoribosome subunits vary greatly in sequence, preventing easy recognition by sequence homology. MRP-S35 (mitochondrial ribosomal protein S35), also known as MDS023, MRPS28 or HDCMD11P, is a 323 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 28S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. Existing as two alternatively spliced isoforms, MRP-S35 is encoded by a gene located on human chromosome 10, which houses over 1,200 genes and comprises nearly 4. 5% of the human genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 28S ribosomal protein S28, mitochondrial; 28S ribosomal protein S35, mitochondrial; mitochondrial ribosomal protein S28; MRP-S28; MRP-S35; MRPS35; S28mt; S35mt; Small ribosomal subunit protein mS35
Gene Aliases: HDCMD11P; MDS023; MRP-S28; MRPS28; MRPS35; PSEC0213
UniProt ID: (Human) P82673
Entrez Gene ID: (Human) 60488
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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