Invitrogen

MSH2 Monoclonal Antibody (1B3=3A2B8C)

MSH2 Antibody in Immunocytochemistry (ICC/IF)

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MSH2 Antibody (MA5-15740) in ICC/IF

Immunofluorescence analysis of MSH2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MSH2 Mouse Monoclonal Antibody (Product # MA5-15740) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at... View More

MSH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

MSH2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with MSH2 siRNA and reduction in signal was observed in Western Blot using MSH2 Monoclonal Antibody (1B3=3A2B8C) (Product # MA5-15740). {KD}

Product Details

MA5-15740

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:100

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1B3=3A2B8C

Immunogen

Purified recombinant fragment of human MSH2 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10983927

Product Specific Information

MA5-15740 targets MSH2 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human and Non-human primate samples.

The MA5-15740 immunogen is purified recombinant fragment of human MSH2 expressed in E. Coli.

MA5-15740 detects MSH2 which has a predicted molecular weight of approximately 105kDa.

Target Information

Plays a role in neurogenesis and neuronal migration. Necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Necessary for cytokinesis and cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Focal amplifications are associated with chromothripsis events and diverse prognoses in gastric cardia adenocarcinoma.

Nature communications

Zhao XK,Xing P,Song X,Zhao M,Zhao L,Dang Y,Lei LL,Xu RH,Han WL,Wang PP,Yang MM,Hu JF,Zhong K,Zhou FY,Han XN,Meng CL,Ji JJ,Chen X,Wang LD

MA5-15740 was used in Immunohistochemistry to identify frequent focal amplifications and ecDNAs in Chinese GCA patient samples, and find focal amplifications in the GCA cohort are associated with the chromothripsis process and may be induced by accumulated DNA damage due to local dietary habits.

Thu Nov 11 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: DNA mismatch repair protein Msh2; hMSH2; Msh2p; mutS homolog 2, colon cancer, nonpolyposis type 1; MutS protein homolog 2

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Gene Aliases: COCA1; FCC1; HNPCC; HNPCC1; LCFS2; MSH2

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UniProt ID: (Human) P43246

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Entrez Gene ID: (Human) 4436

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Function(s)

magnesium ion binding four-way junction DNA binding Y-form DNA binding heteroduplex DNA loop binding double-strand/single-strand DNA junction binding DNA binding double-stranded DNA binding single-stranded DNA binding protein binding ATP binding protein C-terminus binding ATPase activity centromeric DNA binding enzyme binding protein kinase binding mismatched DNA binding guanine/thymine mispair binding dinucleotide insertion or deletion binding single guanine insertion binding single thymine insertion binding dinucleotide repeat insertion binding oxidized purine DNA binding MutLalpha complex binding protein homodimerization activity ADP binding

Process(es)

meiotic mismatch repair in utero embryonic development oxidative phosphorylation DNA repair mismatch repair postreplication repair double-strand break repair meiotic gene conversion cell cycle arrest reciprocal meiotic recombination germ cell development determination of adult lifespan male gonad development response to X-ray response to UV-B somatic hypermutation of immunoglobulin genes somatic recombination of immunoglobulin gene segments B cell mediated immunity B cell differentiation intra-S DNA damage checkpoint intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of neuron apoptotic process maintenance of DNA repeat elements negative regulation of reciprocal meiotic recombination isotype switching negative regulation of DNA recombination positive regulation of helicase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MSH2 Monoclonal Antibody (1B3=3A2B8C)

MSH2 Antibody (MA5-15740) in ICC/IF

Product Details

MA5-15740

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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