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          • Primary Antibodies ›
          • MSH2 Antibodies

          Invitrogen

          MSH2 Monoclonal Antibody (FE11)

          Advanced Verification
          3 Published Figures
          42 References
          View all (52) MSH2 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
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          Cite MSH2 Monoclonal Antibody (FE11)

          • Antibody Testing Data (6)
          • Published Figures (3)
          • Advanced Verification (3)
          MSH2 Antibody in Immunocytochemistry (ICC/IF)
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          MSH2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 12

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          MSH2 Antibody (33-7900) in ICC/IF

          Immunofluorescence analysis of MSH2 was performed using 90% confluent log phase Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MSH2 (FE11) Mouse Monoclonal Antibody (Product # 33-7900) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          MSH2 Antibody in Immunocytochemistry (ICC/IF)
          MSH2 Antibody in Immunocytochemistry (ICC/IF)
          MSH2 Antibody in Immunohistochemistry (IHC)
          MSH2 Antibody in Western Blot (WB)
          MSH2 Antibody in Western Blot (WB)
          MSH2 Antibody in Western Blot (WB)
          MSH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          MSH2 Antibody in Immunohistochemistry (IHC)
          MSH2 Antibody in Immunohistochemistry (IHC)
          MSH2 Antibody
          MSH2 Antibody
          MSH2 Antibody

          Product Details

          33-7900

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-2 µg/mL
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          Assay-dependent
          View 9 publications 9 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 23 publications 23 publications

          Immunocytochemistry (ICC/IF)

          2-3 µg/mL
          View 2 publications 2 publications

          Immunoprecipitation (IP)

          Assay-dependent
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 6 publications 6 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          FE11

          Immunogen

          C-terminal fragment of the human MSH2 protein
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533139

          Target Information

          Plays a role in neurogenesis and neuronal migration. Necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Necessary for cytokinesis and cell proliferation.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: DNA mismatch repair protein Msh2; hMSH2; mismatch repair protein; Msh2p; mutS homolog 2, colon cancer, nonpolyposis type 1; MutS protein homolog 2

          View more View less

          Gene Aliases: AI788990; COCA1; FCC1; HNPCC; HNPCC1; LCFS2; MSH2

          View more View less

          UniProt ID: (Human) P43246, (Mouse) P43247, (Rat) P54275

          View more View less

          Entrez Gene ID: (Human) 4436, (Mouse) 17685, (Rat) 81709

          View more View less

          Function(s)
          magnesium ion binding four-way junction DNA binding Y-form DNA binding heteroduplex DNA loop binding double-strand/single-strand DNA junction binding DNA binding double-stranded DNA binding single-stranded DNA binding protein binding ATP binding protein C-terminus binding ATPase activity centromeric DNA binding enzyme binding protein kinase binding mismatched DNA binding guanine/thymine mispair binding dinucleotide insertion or deletion binding single guanine insertion binding single thymine insertion binding dinucleotide repeat insertion binding oxidized purine DNA binding MutLalpha complex binding protein homodimerization activity ADP binding nucleotide binding damaged DNA binding DNA-dependent ATPase activity single base insertion or deletion binding
          Process(es)
          meiotic mismatch repair in utero embryonic development somatic recombination of immunoglobulin genes involved in immune response oxidative phosphorylation DNA repair mismatch repair postreplication repair double-strand break repair meiotic gene conversion cellular response to DNA damage stimulus cell cycle cell cycle arrest reciprocal meiotic recombination germ cell development determination of adult lifespan male gonad development intrinsic apoptotic signaling pathway in response to DNA damage response to X-ray response to UV-B somatic hypermutation of immunoglobulin genes somatic recombination of immunoglobulin gene segments B cell mediated immunity B cell differentiation intra-S DNA damage checkpoint intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of neuron apoptotic process maintenance of DNA repeat elements negative regulation of reciprocal meiotic recombination isotype switching negative regulation of DNA recombination positive regulation of helicase activity spermatogenesis response to organic cyclic compound response to drug response to amino acid
          It has to be done as per old AB suggested Products section.
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