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Bioss
MSK1/2 Polyclonal Antibody
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Product Details
BS-2995R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human MSK1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
MSK1 is a mitogen and stress activated protein kinase 1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK1 can be activated by ERK1/2 and SAPK2/p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG14 in response to mitogen and stress. Similar to RSK, MSK1 contains two kinase domains (N term and a C term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C terminal kinase domain of MSK1 which is essential for the catalytic activity of the N terminal kinase domain. MSK2 plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 90 kDa ribosomal protein S6 kinase 4; 90 kDa ribosomal protein S6 kinase 5; coiled-coil domain containing 88B; mitogen and stress-activated protein kinase 1; mitogen- and stress-activated protein kinase 2; mitogen- and stress-activated protein kinase-2; Nuclear mitogen- and stress-activated protein kinase 1; Nuclear mitogen- and stress-activated protein kinase 2; Ribosomal protein kinase B; ribosomal protein S6 kinase alpha 4; Ribosomal protein S6 kinase alpha-4; Ribosomal protein S6 kinase alpha-5; ribosomal protein S6 kinase, 90kD, polypeptide 4; ribosomal protein S6 kinase, 90kDa, polypeptide 4; ribosomal protein S6 kinase, 90kDa, polypeptide 5; RLSK; RSK-like protein kinase; RSKB; RSKL; S6K-alpha-4; S6K-alpha-5
Gene Aliases: 1110069D02Rik; 3110005L17Rik; 6330404E13Rik; 90kDa; AI848992; AI854034; Ccdc88b; mMSK2; MSK1; MSK2; MSPK1; RLPK; RPS6KA4; RPS6KA5; RSK-B; S6K-alpha-4
UniProt ID: (Human) O75676, (Human) O75582, (Mouse) Q9Z2B9, (Mouse) Q8C050
Entrez Gene ID: (Human) 8986, (Human) 9252, (Mouse) 56613, (Rat) 361715, (Mouse) 73086, (Rat) 314384
magnesium ion binding
protein serine/threonine kinase activity
ribosomal protein S6 kinase activity
protein binding
ATP binding
histone kinase activity (H3-S10 specific)
mitogen-activated protein kinase p38 binding
protein kinase activity
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
regulation of transcription, DNA-templated
protein phosphorylation
inflammatory response
phosphorylation
histone phosphorylation
positive regulation of histone phosphorylation
positive regulation of histone acetylation
intracellular signal transduction
histone H3-S10 phosphorylation
histone H3-S28 phosphorylation
histone H2A-S1 phosphorylation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of NF-kappaB transcription factor activity
interleukin-1-mediated signaling pathway
negative regulation of cytokine production
stimulatory C-type lectin receptor signaling pathway
epidermal growth factor receptor signaling pathway
axon guidance
positive regulation of CREB transcription factor activity
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