Bethyl Laboratories

MST1-2/STK3-4 Polyclonal Antibody

View all (14) MST1/MST2 antibodies

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Datasheet
Protocols
Questions & Answers

Product Details

IHC-00432

Applications
Tested Dilution
Publications

Immunohistochemistry (IHC)

1:100-1:500
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

C-terminus between residues 450-491 of human Mammalian Sterile 20-like 2 (Serine/Threonine Kinase 3).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.0 to 8.0, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, Zebrafish, X. laevis, X. tropicalis, Turkey, Bovine, Horse, Rabbit, Guinea pig_10141, Panda, Orangutan and Monkey

Target Information

STK3 and STK4 are stress-activated, pro-apoptotic kinases which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. They are key components of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: KB-1458E12.1; kinase responsive to stress 2; Mammalian STE20-like protein kinase 1; Mammalian STE20-like protein kinase 2; mammalian sterile 20-like 1; MST-1; MST-2; MST1/C STK4; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog); Serine/threonine-protein kinase 3; Serine/threonine-protein kinase 4; Serine/threonine-protein kinase Krs-1; Serine/threonine-protein kinase Krs-2; STE20-like kinase MST1; STE20-like kinase MST2

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Gene Aliases: KRS1; KRS2; MST1; MST2; STK3; STK4; YSK3

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UniProt ID: (Human) Q13188, (Human) Q13043

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Entrez Gene ID: (Human) 6788, (Human) 6789

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Function(s)
magnesium ion binding protein kinase activity protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity protein binding ATP binding protein serine/threonine kinase activator activity protein dimerization activity transcription factor binding identical protein binding protein homodimerization activity
Process(es)
neural tube formation endocardium development protein phosphorylation apoptotic process signal transduction central nervous system development negative regulation of cell proliferation signal transduction by protein phosphorylation positive regulation of protein binding hippo signaling intracellular signal transduction positive regulation of apoptotic process positive regulation of fat cell differentiation positive regulation of JNK cascade negative regulation of organ growth protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of protein kinase B signaling primitive hemopoiesis cell differentiation involved in embryonic placenta development regulation of cell differentiation involved in embryonic placenta development positive regulation of protein serine/threonine kinase activity negative regulation of canonical Wnt signaling pathway hepatocyte apoptotic process positive regulation of extrinsic apoptotic signaling pathway via death domain receptors cell morphogenesis patterning of blood vessels positive regulation of protein phosphorylation peptidyl-serine phosphorylation keratinocyte differentiation positive regulation of peptidyl-serine phosphorylation protein autophosphorylation
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