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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • MTAP Antibodies

          Invitrogen

          MTAP Polyclonal Antibody

          View all (26) MTAP antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
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          Cite MTAP Polyclonal Antibody

          • Antibody Testing Data (7)
          MTAP Antibody in Immunocytochemistry (ICC/IF)
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          MTAP Antibody in Immunocytochemistry (ICC/IF)
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          FIGURE: 1 / 7

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          MTAP Antibody (PA5-116435) in ICC/IF

          Immunocytochemical analysis of MTAP in F9 cells using a MTAP polyclonal antibody (Product #PA5-116435). Formalin fixed cells, as seen in green, were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody ( 1:50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          MTAP Antibody in Immunocytochemistry (ICC/IF)
          MTAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          MTAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          MTAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          MTAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          MTAP Antibody in Western Blot (WB)
          MTAP Antibody in Flow Cytometry (Flow)

          Product Details

          PA5-116435

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:100
          -

          Flow Cytometry (Flow)

          1:50-1:100
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein within Human MTAP aa 44-283
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol, 0.2% BSA

          Contains

          0.05% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2901067

          Product Specific Information

          Positive controls: PC-3M cell lysates, F9, rat large intestine tissue, rat kidney tissue, mouse lung tissue, mouse colon tissue, JAR.

          Target Information

          This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: 5'-methylthioadenosine phosphorylase; epididymis luminal protein 249; MeSAdo phosphorylase; MTA phosphorylase; MTAP; MTAPase; S-methyl-5'-thioadenosine phosphorylase

          View more View less

          Gene Aliases: 1300019I21Rik; BDMF; c86fus; Cc1-6; DMSFH; DMSMFH; HEL-249; LGMBF; MSAP; MTAP

          View more View less

          UniProt ID: (Human) Q13126, (Mouse) Q9CQ65

          View more View less

          Entrez Gene ID: (Human) 4507, (Mouse) 66902, (Rat) 298227

          View more View less

          Function(s)
          phosphorylase activity protein binding S-methyl-5-thioadenosine phosphorylase activity catalytic activity transferase activity transferase activity, transferring glycosyl groups transferase activity, transferring pentosyl groups
          Process(es)
          nucleobase-containing compound metabolic process purine ribonucleoside salvage nicotinamide riboside catabolic process L-methionine biosynthetic process from methylthioadenosine methylation response to testosterone purine-containing compound salvage nucleoside metabolic process
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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