Invitrogen

MTCO1 Polyclonal Antibody

MTCO1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

MTCO1 Antibody (PA5-26688) in ICC/IF

Immunocytochemistry analysis of MTCO1 in U-2 OS (human osteosarcoma cell line) cells. Samples were incubated with MTCO1 polyclonal antibody (Product # PA5-26688) using a dilution of 1:25 followed by Dylight® 488-conjugated goat anti-rabbit IgG at a dilution of 1:200 (green). Cells were 4% paraformaldehyde-fixed and 0.1% Triton X-100 permeabilized. Immunofluorescence image showing endoplasmic reticulum staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:100 dilution (red). The nuclear counter stain ... View More

Product Image Gallery

MTCO1 Antibody — FIG. 6. Disruption of differentiation in Tfam-deficient ES cells. (A , B) Tfam and HO-1 mRNA expression by qPCR and Tfam protein by immunoblot in WT and Tfam -/- cell EBs at day 7. Asterisks indicate significant differences in iCORM versus CORM-2, and crosses are WT versus Tfam -/- cells ( p < 0.05) by T -test. CORM-2 is ineffective in Tfam -/- cells. Values are mean +- SD of three independent experiments. (C) Tfam-deficient ES cells cannot differentiate into beating cardiomyocytes and CORM-2 cannot rescue them. Differences between iCORM and CORM-2 treatment and WT and Tfam -/- EBs are significant ( p < 0.05). N = 3-4 experiments per group. (D) Western analysis of alpha-actinin, MLC-2a, and cTnI protein expression in Tfam -/- cells compared with WT cells (tubulin control). (E) Immunoblot analysis of mitochondrial ETC member proteins, COI and COII, and assembly factors, Ndufa1 and Ndufaf4 (porin control). (F) Histogram for quantification of protein in (D , E) . Two-way ANOVA was used for data analysis. Values are mean +- SD of n = 4 independent batches of cells (* p < 0.05 WT iCORM vs. CORM-2; + p < 0.05 WT vs. Tfam -/- ). ETC, electron transport complex. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars {TM}

Product Details

PA5-26688

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:25

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 195-224 amino acids of human COXI

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2544188

Product Specific Information

This antibody is predicted to react with bovine, chicken, mouse, porcine, rat, rabbit and Xenopus and zebrafish based on sequence homology.

Target Information

Cytochrome c oxidase subunit I (CO1 or MTCO1) is 1 of 3 mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al. (2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase (COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant; cultured cells can maintain several mtDNA haplotypes at stable frequencies; the respiratory chain has little spare COX capacity; and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mitochondrial RNA modifications shape metabolic plasticity in metastasis.

Nature

Delaunay S,Pascual G,Feng B,Klann K,Behm M,Hotz-Wagenblatt A,Richter K,Zaoui K,Herpel E,Münch C,Dietmann S,Hess J,Benitah SA,Frye M

PA5-26688 was used in Western Blotting to reveal that site-specific mitochondrial RNA modifications could be therapeutic targets to combat metastasis.

Fri Jul 01 00:00:00 UTC 2022

Augmented Liver Uptake of the Membrane Voltage Sensor Tetraphenylphosphonium Distinguishes Early Fibrosis in a Mouse Model.

Frontiers in physiology

Pandita H,Mezey E,Ganapathy-Kanniappan S

Published figure using MTCO1 polyclonal antibody (Product # PA5-26688) in Western Blot

Mon Jan 10 00:00:00 UTC 2022

Dual-Specificity Phosphatase 1 (DUSP1) Has a Central Role in Redox Homeostasis and Inflammation in the Mouse Cochlea.

Antioxidants (Basel, Switzerland)

Bermúdez-Muñoz JM,Celaya AM,García-Mato Á,Muñoz-Espín D,Rodríguez-de la Rosa L,Serrano M,Varela-Nieto I

PA5-26688 was used in Immunohistochemistry to investigate the link between inflammation and redox imbalance by analysing the cochlear transcriptome in Dusp1-/- mice.

Wed Aug 25 00:00:00 UTC 2021

Deletion of the Mitochondrial Complex-IV Cofactor Heme A:Farnesyltransferase Causes Focal Segmental Glomerulosclerosis and Interferon Response.

The American journal of pathology

Baek JH,Gomez IG,Wada Y,Roach A,Mahad D,Duffield JS

PA5-26688 was used in Western Blotting to determine the impact of mitochondrial dysfunction in focal segmental glomerulosclerosis and secondary innate immune responses, we generated Cre/loxP transgenic mice to generate a loss-of-function deletion mutation of the complex IV assembly cofactor heme A:farnesyltransferase restricted to cells of the developing nephrons.

Sat Dec 01 00:00:00 UTC 2018

Evidence of Oxidative Phosphorylation in Zebrafish Photoreceptor Outer Segments at Different Larval Stages.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Calzia D,Garbarino G,Caicci F,Pestarino M,Manni L,Traverso CE,Panfoli I,Candiani S

PA5-26688 was used in Immunohistochemistry to report fluorescence and Transmission Electron Microscopy colocalization analyses of respiratory complexes I and IV and ATP synthase with zpr3, the rod OS marker, in adult and larval zebrafish retinas.

Sun Jul 01 00:00:00 UTC 2018

RB1 deficiency in triple-negative breast cancer induces mitochondrial protein translation.

The Journal of clinical investigation

Jones RA,Robinson TJ,Liu JC,Shrestha M,Voisin V,Ju Y,Chung PE,Pellecchia G,Fell VL,Bae S,Muthuswamy L,Datti A,Egan SE,Jiang Z,Leone G,Bader GD,Schimmer A,Zacksenhaus E

PA526688 was used in western blot to study the role of RB1 in cancer cell proliferation.

Mon Oct 03 00:00:00 UTC 2016

Depletion of TDP-43 decreases fibril and plaque β-amyloid and exacerbates neurodegeneration in an Alzheimer's mouse model.

Acta neuropathologica

LaClair KD,Donde A,Ling JP,Jeong YH,Chhabra R,Martin LJ,Wong PC

PA526688 was used in western blot to ask if nuclear depletion of TDP-43 contributes to Alzheimer's disease pathogenesis

Thu Dec 01 00:00:00 UTC 2016

Heme Oxygenase-1/Carbon Monoxide System and Embryonic Stem Cell Differentiation and Maturation into Cardiomyocytes.

Antioxidants & redox signaling

Suliman HB,Zobi F,Piantadosi CA

PA5-26688 was used in western blot to assess the impact of the heme oxygenase-1/carbon monoxide system on embryonic stem cell differentiation and cardiomyocyte maturation

Tue Mar 01 00:00:00 UTC 2016

Heme Oxygenase-1/Carbon Monoxide System and Embryonic Stem Cell Differentiation and Maturation into Cardiomyocytes.

Antioxidants & redox signaling

Suliman HB,Zobi F,Piantadosi CA

PA5-26688 was used in western blot to assess the impact of the heme oxygenase-1/carbon monoxide system on embryonic stem cell differentiation and cardiomyocyte maturation

Tue Mar 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: Co1; Cytochrome c oxidase polypeptide I; Cytochrome c oxidase subunit 1

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Gene Aliases: COI; COXI; MT-CO1; MTCO1

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UniProt ID: (Mouse) P00397

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Entrez Gene ID: (Mouse) 17708

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MTCO1 Polyclonal Antibody

MTCO1 Antibody (PA5-26688) in ICC/IF

Product Details

PA5-26688

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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