Invitrogen

MTCO2 Monoclonal Antibody (4B12A5)

Advanced Verification

6 Published Figures

4 References

View all (17) MTCO2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (6)
Advanced Verification (1)

FIGURE: 1 / 7

MTCO2 Antibody (459150) in WB

PloS one 2011 - Figure 1 Analysis of puf3Delta Yeast Strains. Analysis of wild-type (wt) and an isogenic puf3Delta strains are shown. (A) Mitochondrial oxygen consumption assayed at 12 hours (log phase), 24 hours (early stationary phase), and 48 hours post-inoculation (1 day into stationary phase). The bars represent the mean +- SD with p-values as indicated (*<0.05, **<0.01, ***<0.001). Statistical... View More

Product Image Gallery

MTCO2 Antibody — Figure 6 Comparison of mitochondrial parameters in wild-type and puf3Delta mutant strains treated with the TORC1 inhibitor rapamycin. (A) Mitochondrial oxygen consumption of cells treated with rapamycin during early log phase and analyzed during late log phase. (B) Western blot analysis of protein extracts derived from late log phase cultures (representative of three biological replicates). (C) Steady-state levels of COX17 , COX12 , and RIP1 transcripts in late log phase. The values indicate the mean +/- SD with p-values indicated as described in the legend of Figure 1 . {TM}

Product Details

459150

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 4 publications 4 publications

Product Specifications

Species Reactivity

Yeast

Published species

Yeast

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

4B12A5

Immunogen

Yeast cytochrome c oxidase subunit II

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

IgG fraction

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2532228

Product Specific Information

Positive control: Mitochondria from yeast membrane extract

Target Information

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pbp1 associates with Puf3 and promotes translation of its target mRNAs involved in mitochondrial biogenesis.

PLoS genetics

van de Poll F,Sutter BM,Acoba MG,Caballero D,Jahangiri S,Yang YS,Lee CD,Tu BP

459150 was used in Western Blot to reveal a key role for Pbp1-containing assemblies in enabling the translation of mRNAs critical for mitochondrial biogenesis and respiration.

Mon May 01 00:00:00 UTC 2023

DarT-mediated mtDNA damage induces dynamic reorganization and selective segregation of mitochondria.

The Journal of cell biology

Dua N,Seshadri A,Badrinarayanan A

459150 was used in Western Blot to suggest that, under damage, partitioning of defective mtDNA and organelle are de-coupled, with emphasis on mitochondrial segregation independent of its DNA.

Mon Oct 03 00:00:00 UTC 2022

Mapping the Saccharomyces cerevisiae Spatial Proteome with High Resolution Using hyperLOPIT.

Methods in molecular biology (Clifton, N.J.)

Nightingale DJH,Oliver SG,Lilley KS

459150 was used in Western Blotting to describe a protocol for the unbiased and high-throughput study of protein subcellular localization in Saccharomyces cerevisiae via hyperLOPIT.

Mon Jun 22 00:00:00 UTC 2020

Repression of mitochondrial translation, respiration and a metabolic cycle-regulated gene, SLF1, by the yeast Pumilio-family protein Puf3p.

PloS one

Chatenay-Lapointe M,Shadel GS

459150 was used in western blot to study the coordination of gene expression between the nuclear and mitochondrial genomes.

Wed Oct 05 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: Co2; cyclooxygenase; Cytochrome c oxidase polypeptide II; Cytochrome c oxidase subunit 2

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Gene Aliases: COX2; OXI1; OXII; Q0250

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UniProt ID: (Baker's yeast) P00410

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Entrez Gene ID: (Baker's yeast) 854622

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Function(s)

cytochrome-c oxidase activity copper ion binding oxidoreductase activity metal ion binding

Process(es)

mitochondrial electron transport, cytochrome c to oxygen aerobic respiration electron transport chain oxidation-reduction process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MTCO2 Monoclonal Antibody (4B12A5)

MTCO2 Antibody (459150) in WB

Product Details

459150

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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