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OriGene
MTDH Monoclonal Antibody (OTI2A6), TrueMAB™
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FIGURE: 1 / 1
MTDH Antibody (CF804132) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTDH (RC207238, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-MTDH. Positive lysates LY403614 (100 µg) and LC403614 (20 µg) can be purchased separately from OriGene.
Product Details
CF804132
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI2A6
Immunogen
Human recombinant protein fragment corresponding to amino acids 70-582 of human MTDH produced in SF9 cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3D3/LYRIC; AEG-1; astrocyte elevated gene 1; Astrocyte elevated gene-1 protein; LYRIC/ MTDH; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein; Protein LYRIC
Gene Aliases: 3D3; AEG-1; AEG1; LYRIC; LYRIC/3D3; MTDH
UniProt ID: (Human) Q86UE4
Entrez Gene ID: (Human) 92140
negative regulation of transcription from RNA polymerase II promoter
positive regulation of autophagy
lipopolysaccharide-mediated signaling pathway
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of angiogenesis
positive regulation of NF-kappaB transcription factor activity
positive regulation of protein kinase B signaling
bicellular tight junction assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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