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Invitrogen
MTHFD2 Polyclonal Antibody
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MTHFD2 Antibody (PA5-30407) in IHC (P)
MTHFD2 Polyclonal Antibody detects MTHFD2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. MTHFD2 stained by MTHFD2 Polyclonal Antibody (Product # PA5-30407) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Product Details
PA5-30407
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 141 and 350 of Human MTHFD2
Conjugate
Form
Liquid
Concentration
0.61 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2547881
Product Specific Information
Recommended positive controls: A549, HeLa, HepG2, HCT116.
Predicted reactivity: Mouse (96%), Rat (95%), Zebrafish (85%), Xenopus laevis (89%), Chicken (85%), Bovine (94%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial; methylenetetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase; methylenetetrahydrofolate dehydrogenase 2; NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase
Gene Aliases: AW558851; MTHFD2; NMDMC
UniProt ID: (Human) P13995, (Mouse) P18155
Entrez Gene ID: (Human) 10797, (Mouse) 17768, (Rat) 680308
magnesium ion binding
formate-tetrahydrofolate ligase activity
methenyltetrahydrofolate cyclohydrolase activity
methylenetetrahydrofolate dehydrogenase (NAD+) activity
methylenetetrahydrofolate dehydrogenase (NADP+) activity
phosphate ion binding
catalytic activity
oxidoreductase activity
hydrolase activity
molecular_function
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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