Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
OriGene
MTHFR Monoclonal Antibody (OTI2B4), TrueMAB™
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 1
MTHFR Antibody (CF812671) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTHFR (RC208588, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-MTHFR. Positive lysates LY416960 (100 µg) and LC416960 (20 µg) can be purchased separately from OriGene.
Product Details
CF812671
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI2B4
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-230 of human MTHFR produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
The protein encoded by this gene catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular disease, neural tube defects, colon cancer and acute leukemia, and mutations in this gene are associated with methylenetetrahydrofolate reductase deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 5,10-methylenetetrahydrofolate reductase (NADPH); methylenetetrahydrofolate reductase; Methylenetetrahydrofolate reductase (NADPH); RP11-56N19.4
Gene Aliases: AI323986; MTHFR
UniProt ID: (Human) P42898, (Mouse) Q9WU20
Entrez Gene ID: (Human) 4524, (Rat) 362657, (Mouse) 17769
response to hypoxia
cellular amino acid metabolic process
methionine metabolic process
blood circulation
regulation of histone methylation
response to vitamin B2
tetrahydrofolate interconversion
response to drug
response to amino acid
S-adenosylmethionine metabolic process
folic acid metabolic process
homocysteine metabolic process
response to folic acid
oxidation-reduction process
response to interleukin-1
heterochromatin maintenance
one-carbon metabolic process
methionine biosynthetic process
tetrahydrofolate metabolic process
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support