Proteintech

MTO1 Polyclonal Antibody

MTO1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

MTO1 Antibody (15650-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA) fixed MCF-7 cells using 15650-1-AP (MTO1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Product Image Gallery

Product Details

15650-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

1:50-1:500

Immunoprecipitation (IP)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MTO1 Fusion Protein Ag8192 (368-717 aa encoded by BC005808)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LEKAKVIQP DGVLLLLPRM ECNGAISAHH NLPLPGYGVQ YDYLDPRQIT PSLETHLVQR LFFAGQINGT TGYEEAAAQG VIAGINASLR VSRKPPFVVS RTEGYIGVLI DDLTTLGTSE PYRMFTSRVE FRLSLRPDNA DSRLTLRGYK DAGCVSQQRY ERACWMKSSL EEGISVLKSI EFLSSKWKKL IPEASISTSR SLPVRALDVL KYEEVDMDSL AKAVPEPLKK YTKCRELAER LKIEATYESV LFHQLQEIKG VQQDEALQLP KDLDYLTIRD VSLSHEVREK LHFSRPQTIG AASRIPGVTP AAIINLLRFV KTTQRRQSAM NESSKTDQYL CDADRLQERE L (368-717 aa encoded by BC005808)

Target Information

MTO1 encodes a mitochondrial protein thought to be involved in mitochondrial tRNA modification. The encoded protein may also play a role in the expression of the non-syndromic and aminoglycoside-induced deafness phenotypes associated with a specific mutation in the mitochondrial 12S rRNA gene. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Restoration of mitochondrial function through activation of hypomodified tRNAs with pathogenic mutations associated with mitochondrial diseases.

Nucleic acids research

Tomoda E,Nagao A,Shirai Y,Asano K,Suzuki T,Battersby BJ,Suzuki T

Fri Aug 11 00:00:00 UTC 2023

The human tRNA taurine modification enzyme GTPBP3 is an active GTPase linked to mitochondrial diseases.

Nucleic acids research

Peng GX,Zhang Y,Wang QQ,Li QR,Xu H,Wang ED,Zhou XL

Thu Mar 18 00:00:00 UTC 2021

The MELAS mutation m.3243A>G alters the expression of mitochondrial tRNA fragments.

Biochimica et biophysica acta. Molecular cell research

Meseguer S,Navarro-González C,Panadero J,Villarroya M,Boutoual R,Sánchez-Alcázar JA,Armengod ME

Sun Sep 01 00:00:00 UTC 2019

RNA modification landscape of the human mitochondrial tRNA^Lys regulates protein synthesis.

Nature communications

Richter U,Evans ME,Clark WC,Marttinen P,Shoubridge EA,Suomalainen A,Wredenberg A,Wedell A,Pan T,Battersby BJ

Thu Sep 27 00:00:00 UTC 2018

RNA modification landscape of the human mitochondrial tRNA^Lys regulates protein synthesis.

Nature communications

Richter U,Evans ME,Clark WC,Marttinen P,Shoubridge EA,Suomalainen A,Wredenberg A,Wedell A,Pan T,Battersby BJ

Thu Sep 27 00:00:00 UTC 2018

Defects in the mitochondrial-tRNA modification enzymes MTO1 and GTPBP3 promote different metabolic reprogramming through a HIF-PPARγ-UCP2-AMPK axis.

Scientific reports

Boutoual R,Meseguer S,Villarroya M,Martín-Hernández E,Errami M,Martín MA,Casado M,Armengod ME

Thu Jan 18 00:00:00 UTC 2018

Defects in the mitochondrial-tRNA modification enzymes MTO1 and GTPBP3 promote different metabolic reprogramming through a HIF-PPARγ-UCP2-AMPK axis.

Scientific reports

Boutoual R,Meseguer S,Villarroya M,Martín-Hernández E,Errami M,Martín MA,Casado M,Armengod ME

Thu Jan 18 00:00:00 UTC 2018

microRNA-mediated differential expression of TRMU, GTPBP3 and MTO1 in cell models of mitochondrial-DNA diseases.

Scientific reports

Meseguer S,Boix O,Navarro-González C,Villarroya M,Boutoual R,Emperador S,García-Arumí E,Montoya J,Armengod ME

Mon Jul 24 00:00:00 UTC 2017

Severe respiratory complex III defect prevents liver adaptation to prolonged fasting.

Journal of hepatology

Kremer LS,L'hermitte-Stead C,Lesimple P,Gilleron M,Filaut S,Jardel C,Haack TB,Strom TM,Meitinger T,Azzouz H,Tebib N,Ogier de Baulny H,Touati G,Prokisch H,Lombès A

Mon Aug 01 00:00:00 UTC 2016

Severe respiratory complex III defect prevents liver adaptation to prolonged fasting.

Journal of hepatology

Kremer LS,L'hermitte-Stead C,Lesimple P,Gilleron M,Filaut S,Jardel C,Haack TB,Strom TM,Meitinger T,Azzouz H,Tebib N,Ogier de Baulny H,Touati G,Prokisch H,Lombès A

Mon Aug 01 00:00:00 UTC 2016

The ROS-sensitive microRNA-9/9* controls the expression of mitochondrial tRNA-modifying enzymes and is involved in the molecular mechanism of MELAS syndrome.

Human molecular genetics

Meseguer S,Martínez-Zamora A,García-Arumí E,Andreu AL,Armengod ME

Thu Jan 01 00:00:00 UTC 2015

The ROS-sensitive microRNA-9/9* controls the expression of mitochondrial tRNA-modifying enzymes and is involved in the molecular mechanism of MELAS syndrome.

Human molecular genetics

Meseguer S,Martínez-Zamora A,García-Arumí E,Andreu AL,Armengod ME

Thu Jan 01 00:00:00 UTC 2015

Altered 2-thiouridylation impairs mitochondrial translation in reversible infantile respiratory chain deficiency.

Human molecular genetics

Boczonadi V,Smith PM,Pyle A,Gomez-Duran A,Schara U,Tulinius M,Chinnery PF,Horvath R

Fri Nov 15 00:00:00 UTC 2013

Altered 2-thiouridylation impairs mitochondrial translation in reversible infantile respiratory chain deficiency.

Human molecular genetics

Boczonadi V,Smith PM,Pyle A,Gomez-Duran A,Schara U,Tulinius M,Chinnery PF,Horvath R

Fri Nov 15 00:00:00 UTC 2013

Mutations of the mitochondrial-tRNA modifier MTO1 cause hypertrophic cardiomyopathy and lactic acidosis.

American journal of human genetics

Ghezzi D,Baruffini E,Haack TB,Invernizzi F,Melchionda L,Dallabona C,Strom TM,Parini R,Burlina AB,Meitinger T,Prokisch H,Ferrero I,Zeviani M

Fri Jun 08 00:00:00 UTC 2012

Mutations of the mitochondrial-tRNA modifier MTO1 cause hypertrophic cardiomyopathy and lactic acidosis.

American journal of human genetics

Ghezzi D,Baruffini E,Haack TB,Invernizzi F,Melchionda L,Dallabona C,Strom TM,Parini R,Burlina AB,Meitinger T,Prokisch H,Ferrero I,Zeviani M

Fri Jun 08 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: CGI02; homolog of yeast Mto1; mitochondrial MTO1-3; mitochondrial translation optimization 1 homolog; Protein MTO1 homolog, mitochondrial

View more View less

Gene Aliases: CGI-02; COXPD10; MTO1

View more View less

UniProt ID: (Human) Q9Y2Z2

View more View less

Entrez Gene ID: (Human) 25821

View more View less

Function(s)

oxidoreductase activity poly(A) RNA binding flavin adenine dinucleotide binding

Process(es)

tRNA methylation oxidation-reduction process mitochondrial tRNA wobble uridine modification

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

MTO1 Polyclonal Antibody

MTO1 Antibody (15650-1-AP) in ICC/IF

Product Details

15650-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!