Proteintech

MTR Polyclonal Antibody

MTR Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

MTR Antibody (25896-1-AP) in ICC/IF

Immunofluorescent analysis of A375 cells using 25896-1-AP (MTR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MTR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

25896-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MTR Fusion Protein Ag23156 (917-1265 aa encoded by BC130616)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: RQGHYESLK ERRYLPLSQA RKSGFQMDWL SEPHPVKPTF IGTQVFEDYD LQKLVDYIDW KPFFDVWQLR GKYPNRGFPK IFNDKTVGGE ARKVYDDAHN MLNTLISQKK LRARGVVGFW PAQSIQDDIH LYAEAAVPQA AEPIATFYGL RQQAEKDSAS TEPYYCLSDF IAPLHSGIRD YLGLFAVACF GVEELSKAYE DDGDDYSSIM VKALGDRLAE AFAEELHERV RRELWAYCGS EQLDVADLRR LRYKGIRPAP GYPSQPDHTE KLTMWRLADI EQSTGIRLTE SLAMAPASAV SGLYFSNLKS KYFAVGKISK DQVEDYALRK NISVAEVEKW LGPILGYDTD (917-1265 aa encoded by BC130616)

Target Information

MTR encodes the enzyme 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Reduced methionine synthase expression results in uracil accumulation in mitochondrial DNA and impaired oxidative capacity.

PNAS nexus

Heyden KE,Fiddler JL,Xiu Y,Malysheva OV,Handzlik MK,Phinney WN,Stiles L,Stabler SP,Metallo CM,Caudill MA,Field MS

Sat Apr 01 00:00:00 UTC 2023

Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.

Cell reports

Sayles NM,Southwell N,McAvoy K,Kim K,Pesini A,Anderson CJ,Quinzii C,Cloonan S,Kawamata H,Manfredi G

Tue Mar 08 00:00:00 UTC 2022

Linkage of methionine addiction, histone lysine hypermethylation, and malignancy.

iScience

Yamamoto J,Inubushi S,Han Q,Tashiro Y,Sugisawa N,Hamada K,Aoki Y,Miyake K,Matsuyama R,Bouvet M,Clarke SG,Endo I,Hoffman RM

Fri Apr 15 00:00:00 UTC 2022

Mild Choline Deficiency and MTHFD1 Synthetase Deficiency Interact to Increase Incidence of Developmental Delays and Defects in Mice.

Nutrients

Christensen KE,Malysheva OV,Carlin S,Matias F,MacFarlane AJ,Jacobs RL,Caudill MA,Rozen R

Tue Dec 28 00:00:00 UTC 2021

Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Journal of pineal research

Cui L,Zhao X,Jin Z,Wang H,Yang SF,Hu S

Wed Dec 01 00:00:00 UTC 2021

mTORC1 stimulates cell growth through SAM synthesis and m⁶A mRNA-dependent control of protein synthesis.

Molecular cell

Villa E,Sahu U,O'Hara BP,Ali ES,Helmin KA,Asara JM,Gao P,Singer BD,Ben-Sahra I

Thu May 20 00:00:00 UTC 2021

Methionine synthase supports tumour tetrahydrofolate pools.

Nature metabolism

Ghergurovich JM,Xu X,Wang JZ,Yang L,Ryseck RP,Wang L,Rabinowitz JD

Mon Nov 01 00:00:00 UTC 2021

Exploring the protective effects of schizandrol A in acute myocardial ischemia mice by comprehensive metabolomics profiling integrated with molecular mechanism studies.

Acta pharmacologica Sinica

Lai Q,Yuan GY,Wang H,Liu ZL,Kou JP,Yu BY,Li F

Sat Aug 01 00:00:00 UTC 2020

Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation.

Cell metabolism

Zhu J,Berisa M,Schwörer S,Qin W,Cross JR,Thompson CB

Tue Nov 05 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: 5-methyltetrahydrofolate--homocysteine methyltransferase; 5-methyltetrahydrofolate-homocysteine methyltransferase 1; Cobalamin-dependent methionine synthase; FLJ33168; FLJ43216; FLJ45386; Methionine synthase; MS; Vitamin-B12 dependent methionine synthase

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Gene Aliases: AI894170; cblG; D830038K18Rik; HMAG; MS; MTR

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UniProt ID: (Human) Q99707, (Mouse) A6H5Y3

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Entrez Gene ID: (Human) 4548, (Mouse) 238505

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Function(s)

protein binding zinc ion binding methionine synthase activity S-adenosylmethionine-homocysteine S-methyltransferase activity cobalamin binding folic acid binding methyltransferase activity amino acid binding transferase activity metal ion binding

Process(es)

sulfur amino acid metabolic process nervous system development methionine biosynthetic process cobalamin metabolic process axon regeneration methylation pteridine-containing compound metabolic process response to axon injury cellular response to nitric oxide protein methylation methionine metabolic process cellular amino acid biosynthetic process cellular metabolic process tetrahydrofolate metabolic process homocysteine metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MTR Polyclonal Antibody

MTR Antibody (25896-1-AP) in ICC/IF

Product Details

25896-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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