Invitrogen

MUC2 Monoclonal Antibody (996/1)

7 Published Figures

17 References

View all (40) MUC2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (7)
Published Figures (7)

MUC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 14

MUC2 Antibody (MA5-12345) in IHC (P)

Immunohistochemistry analysis of Mucin 2 showing staining in the cytoplasm of paraffin-embedded human colon tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, w... View More

MUC2 Antibody in Immunocytochemistry (ICC/IF)

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Product Details

MA5-12345

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:500

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 9 publications 9 publications

Flow Cytometry (Flow)

0.5-1 µg/test

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

996/1

Immunogen

MUC2 tandem repeat peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

0.2% BSA

Contains

?0.1% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10975230

Product Specific Information

MA5-12345 targets Mucin 2 in IHC (P), ICC/IF and FACS applications and shows reactivity with human and mouse samples.

The MA5-12345 immunogen is mUC2 tandem repeat peptide.

Target Information

Secreted epithelial mucins are large macromolecules which exhibit extreme polydispersity. Mucin 2 is the major intestinal mucin. O-glycans are attached to MUC2 in a potentially diverse arrangement, which is crucial for their interaction with endogeneous and exogeneous lectins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Novel 3D Flipwell system that models gut mucosal microenvironment for studying interactions between gut microbiota, epithelia and immunity.

Scientific reports

Beamer MA,Zamora C,Nestor-Kalinoski AL,Fernando V,Sharma V,Furuta S

MA5-12345 was used in Flow Cytometry, Immunohistochemistry (Paraffin), Immunocytochemistry to demonstrate that the novel co-culture may serve as a robust in vitro system to recapitulate the effects of pharmacological agents on the gut mucosal microenvironment, and could potentially be expanded to test the effects outside the gut.

Tue Jan 17 00:00:00 UTC 2023

Glutamine promotes O-GlcNAcylation of G6PD and inhibits AGR2 S-glutathionylation to maintain the intestinal mucus barrier in burned septic mice.

Redox biology

Wu D,Su S,Zha X,Wei Y,Yang G,Huang Q,Yang Y,Xia L,Fan S,Peng X

MA5-12345 was used in Immunocytochemistry to demonstrate that the central mechanisms of glutamine in promoting MUC2 maturation and maintaining the intestinal mucus barrier are the enhancement of glucose-6-phosphate dehydrogenase (G6PD) glycosylation and inhibition of anterior gradient-2 (AGR2) S-glutathionylation.

Wed Feb 01 00:00:00 UTC 2023

Glutamine promotes O-GlcNAcylation of G6PD and inhibits AGR2 S-glutathionylation to maintain the intestinal mucus barrier in burned septic mice.

Redox biology

Wu D,Su S,Zha X,Wei Y,Yang G,Huang Q,Yang Y,Xia L,Fan S,Peng X

Wed Feb 01 00:00:00 UTC 2023

A platform to reproducibly evaluate human colon permeability and damage.

Scientific reports

Marr EE,Mulhern TJ,Welch M,Keegan P,Caballero-Franco C,Johnson BG,Kasaian M,Azizgolshani H,Petrie T,Charest J,Wiellette E

MA5-12345 was used in Immunocytochemistry to develop an Inflammatory Bowel Disease-relevant model through cytokine induced damage that can support studies of disease mechanisms and putative therapeutics.

Thu Jun 01 00:00:00 UTC 2023

Human intestinal bitter taste receptors regulate innate immune responses and metabolic regulators in obesity.

The Journal of clinical investigation

Liszt KI,Wang Q,Farhadipour M,Segers A,Thijs T,Nys L,Deleus E,Van der Schueren B,Gerner C,Neuditschko B,Ceulemans LJ,Lannoo M,Tack J,Depoortere I

Published figure using MUC2 monoclonal antibody (Product # MA5-12345) in Immunocytochemistry

Tue Feb 01 00:00:00 UTC 2022

A Novel In Vitro Membrane Permeability Methodology Using Three-dimensional Caco-2 Tubules in a Microphysiological System Which Better Mimics In Vivo Physiological Conditions.

Journal of pharmaceutical sciences

Hagiwara Y,Kumagai H,Ouwerkerk N,Gijzen L,Annida R,Bokkers M,van Vught R,Yoshinari K,Katakawa Y,Motonaga K,Tajiri T

MA5-12345 was used in Immunohistochemistry to develop an in vitro drug permeability methodology which mimics the gastrointestinal environment more accurately than conventional 2D methodologies through 3D Caco-2 tubules using a microphysiological system.

Sat Jan 01 00:00:00 UTC 2022

Small proline-rich protein 2A is a gut bactericidal protein deployed during helminth infection.

Science (New York, N.Y.)

Hu Z,Zhang C,Sifuentes-Dominguez L,Zarek CM,Propheter DC,Kuang Z,Wang Y,Pendse M,Ruhn KA,Hassell B,Behrendt CL,Zhang B,Raj P,Harris-Tryon TA,Reese TA,Hooper LV

MA5-12345 was used in Immunocytochemistry-immunoflourescence to show that SPRR2A is a distinctive AMP triggered by type 2 immunity that protects the intestinal barrier during helminth infection.

Fri Nov 05 00:00:00 UTC 2021

Hyperglycemia minimally alters primary self-renewing human colonic epithelial cells while TNFα-promotes severe intestinal epithelial dysfunction.

Integrative biology : quantitative biosciences from nano to macro

Dutton JS,Hinman SS,Kim R,Attayek PJ,Maurer M,Sims CS,Allbritton NL

MA5-12345 was used in Immunocytochemistry-immunoflourescence to suggest that TNFα acted as the dominant modulator of the epithelium relative to glucose, and that control of inflammation rather than glucose may be key to maintaining intestinal homeostasis.

Tue Jun 15 00:00:00 UTC 2021

Parechovirus A Infection of the Intestinal Epithelium: Differences Between Genotypes A1 and A3.

Frontiers in cellular and infection microbiology

García-Rodríguez I,van Eijk H,Koen G,Pajkrt D,Sridhar A,Wolthers KC

Published figure using MUC2 monoclonal antibody (Product # MA5-12345) in Immunocytochemistry

Wed Nov 24 00:00:00 UTC 2021

Evaluation of a Live Attenuated S. sonnei Vaccine Strain in the Human Enteroid Model.

Pathogens (Basel, Switzerland)

Pilla G,Wu T,Grassel C,Moon J,Foulke-Abel J,Tang CM,Barry EM

MA5-12345 was used in Immunocytochemistry to evaluate a plasmid-stabilized Shigella sonnei live attenuated vaccine strain and a multivalent derivative which expresses antigens from enterotoxigenic Escherichia coli.

Wed Aug 25 00:00:00 UTC 2021

Evaluation of a Live Attenuated S. sonnei Vaccine Strain in the Human Enteroid Model.

Pathogens (Basel, Switzerland)

Pilla G,Wu T,Grassel C,Moon J,Foulke-Abel J,Tang CM,Barry EM

Wed Aug 25 00:00:00 UTC 2021

Metformin Exerts Anti-inflammatory and Mucus Barrier Protective Effects by Enriching Akkermansia muciniphila in Mice With Ulcerative Colitis.

Frontiers in pharmacology

Ke H,Li F,Deng W,Li Z,Wang S,Lv P,Chen Y

MA5-12345 was used in Immunohistochemistry to provide evidence that A. muciniphila as a probiotic has potential benefits for ulcerative colitis.

Fri Oct 22 00:00:00 UTC 2021

Metformin Exerts Anti-inflammatory and Mucus Barrier Protective Effects by Enriching Akkermansia muciniphila in Mice With Ulcerative Colitis.

Frontiers in pharmacology

Ke H,Li F,Deng W,Li Z,Wang S,Lv P,Chen Y

MA5-12345 was used in Immunohistochemistry to provide evidence that A. muciniphila as a probiotic has potential benefits for ulcerative colitis.

Fri Oct 22 00:00:00 UTC 2021

Host Specificity of Flagellins from Segmented Filamentous Bacteria Affects Their Patterns of Interaction with Mouse Ileal Mucosal Proteins.

Applied and environmental microbiology

Chen H,Yin Y,Wang Y,Wang X,Xiang C

MA5-12345 was used in Immunocytochemistry to investigate the gene diversity and host specificity of segmented filamentous bacterial flagellin genes.

Fri Sep 15 00:00:00 UTC 2017

Early Gastric Cancer-Like Advanced Gastric Cancer versus Advanced Gastric Cancer-Like Early Gastric Cancer.

Clinical endoscopy

Park HS,Lee SY,Hong SN,Kim JH,Sung IK,Park HS,Shim CS,Jin CJ

MA5-12345 was used in immunohistochemistry to study endoscopic differences between early gastric cancer that resembles advanced gastric cancer and advanced gastric cancer that resembles early gastric cancer

Fri Mar 01 00:00:00 UTC 2013

MUC5AC, cytokeratin 20 and HER2 expression and K-RAS mutations within mucinogenic growth in congenital pulmonary airway malformations.

Histopathology

Rossi G,Gasser B,Sartori G,Migaldi M,Costantini M,Mengoli MC,Piccioli S,Cavazza A,Rivasi F

MA5-12345 was used in immunohistochemistry to investigate the changes of gene expression in congenital pulmonary airway malformations

Fri Jun 01 00:00:00 UTC 2012

Change of mucosal color in early gastric cancer.

Journal of digestive diseases

Lee HH,Lee SY,Yoon HY,Hong SN,Kim JH,Sung IK,Park HS,Shim CS,Han HS

MA5-12345 was used in immunohistochemistry to report on the diagnostic value of mucosal color changes in early gastric cancer

Mon Oct 01 00:00:00 UTC 2012

Unclassified mucin phenotype of gastric adenocarcinoma exhibits the highest invasiveness.

Journal of gastroenterology and hepatology

Han HS,Lee SY,Lee KY,Hong SN,Kim JH,Sung IK,Park HS,Jin CJ,Min YI

MA5-12345 was used in immunohistochemistry to investigate phenotypic mucin expression in human gastric adenocarcinoma

Wed Apr 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: colonic mucin; Intestinal mucin-2; MCM; MUC-2; muc2; mucin 2, intestinal/tracheal; Mucin-2; mucin-like protein; secreted gel-forming mucin

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Gene Aliases: 2010015E03Rik; MCM; MLP; MUC-2; MUC2; SMUC; wnn

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UniProt ID: (Human) Q02817

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Entrez Gene ID: (Human) 4583, (Mouse) 17831

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Function(s)

protein binding

Process(es)

O-glycan processing maintenance of gastrointestinal epithelium epithelial cell development apoptotic process negative regulation of cell proliferation negative regulation of cell migration positive regulation of apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MUC2 Monoclonal Antibody (996/1)

MUC2 Antibody (MA5-12345) in IHC (P)

Product Details

MA5-12345

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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