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MUC20 Antibody (PA5-90867) in WB
Western blot analysis of MUC20 in extracts of various cell lines. Samples were incubated with MUC20 Polyclonal antibody (Product # PA5-90867) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-90867
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human MUC20 (NP_6898863)
Conjugate
Form
Liquid
Concentration
2.06 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806267
Product Specific Information
Immunogen sequence: PNFMVLIATS VETSAASGSP EGAGMTTVQT ITGSDPEEAI FDTLCTDDSS EEAKTLTMDI LTLAHTSTEA KGLSSESSAS SDGPHPVITP SRASESSASS D; Positive Samples: 293T, A-549, DU145, LO2, U-251MG, HT-29, Mouse kidney, Mouse lung, Rat kidney; Cellular Location: Apical cell membrane, Basolateral cell membrane, Cell projection, microvillus membrane
Target Information
This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The shorter isoform expressed by this gene is localized to the plasma membrane, whereas the longer isoform might be secreted. The C terminus of this protein associates with the multifunctional docking site of the met proto-oncogene and suppresses activation of some downstream met signaling cascades. The protein features a tandem repeat domain that varies between 2 and 6 copies in most individuals. The allele represented in the human reference assembly contains 12 copies. Multiple transcript variants encoding different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MUC-20; Mucin-20; transmembrane mucin MUC20S
Gene Aliases: BC026367; KIAA1359; MUC-20; MUC20; UNQ2782/PRO7170
UniProt ID: (Human) Q8N307, (Mouse) Q8BUE7
Entrez Gene ID: (Human) 200958, (Mouse) 224116, (Rat) 303886
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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