Invitrogen

MUC5B Polyclonal Antibody

MUC5B Antibody in Immunocytochemistry (ICC/IF)

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MUC5B Antibody (PA5-82342) in ICC/IF

Immunofluorecent analysis of MUC5B in human cell line A549 using MUC5B Polyclonal Antibody (Product # PA5-82342). Staining shows localization to vesicles.

MUC5B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

MUC5B Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of MUC5B demonstrates antibody specificity. Immunohistochemical analysis of MUC5B using anti-MUC5B Polyclonal Antibody (Product #PA5-82342), shows significant staining of MUC5B in colon and shows minimal or weak staining in skeletal muscle tissues. The relative expression levels of MUC5B within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-82342

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human MUC5B. Recombinant protein control fragment (Product #RP-88563).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.11 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2789501

Product Specific Information

Immunogen sequence: CTCTYEDRTY SYQDVIYNTT DGLGACLIAI CGSNGTIIRK AVACPGTPAT TPFTFTTAWV PHSTTSPALP VSTVCVREVC RWSSWYNGHR PEPGLGGGDF ETFENLRQRG YQVCPVLA

Target Information

Mucins (MUCs) are heavily glycosylated glycoproteins that are synthesized either as membrane-bound or secreted proteins. Mucins are noteworthy because they have an aberrant expression profile in various malignancies. Mucins normally play a protective role for epithelial tissues and are expressed in the gastrointestinal, respiratory, and genito-urinary tracts. Mucins may also be involved in the renewal and differentiation of the epithelium, and the modulation of cell adhesion and cell signaling. MUC5B is thought to play a vital role in maintaining oral health, and may also be involved in respiratory and oral disease.2 MUC5B expression has been studied in gastric carcinoma as well as chronic obstructive pulmonary disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A comparative study of in vitro air-liquid interface culture models of the human airway epithelium evaluating cellular heterogeneity and gene expression at single cell resolution.

Respiratory research

Prescott RA,Pankow AP,de Vries M,Crosse KM,Patel RS,Alu M,Loomis C,Torres V,Koralov S,Ivanova E,Dittmann M,Rosenberg BR

PA5-82342 was used in Immunohistochemistry-immunofluorescence to support BCi-NS1.1-derived HAE cultures as a valuable tool for the study of airway infectious disease.

Mon Aug 28 00:00:00 UTC 2023

Engineered tRNAs suppress nonsense mutations in cells and in vivo.

Nature

Albers S,Allen EC,Bharti N,Davyt M,Joshi D,Perez-Garcia CG,Santos L,Mukthavaram R,Delgado-Toscano MA,Molina B,Kuakini K,Alayyoubi M,Park KJ,Acharya G,Gonzalez JA,Sagi A,Birket SE,Tearney GJ,Rowe SM,Manfredi C,Hong JS,Tachikawa K,Karmali P,Matsuda D,Sorscher EJ,Chivukula P,Ignatova Z

PA5-82342 was used in Immunohistochemistry to provide a framework for the development of tRNA-based therapies with a high molecular safety profile and high efficacy in targeted PTC suppression.

Thu Jun 01 00:00:00 UTC 2023

Quantification of increased MUC5AC expression in airway mucus of smoker using an automated image-based approach.

Microscopy research and technique

Groiss S,Somvilla I,Daxböck C,Fuchs J,Lang-Olip I,Stiegler P,Leber B,Liegl-Atzwanger B,Brislinger D

PA5-82342 was used in Immunohistochemistry-immunofluorescence to present a simple, image-based, and high-processing computational method that allows determining the ratio of MUC5AC and MUC5B within the overall airway mucus while providing information on their spatial distribution.

Sat Jan 01 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: Cervical mucin; cervical mucin MUC5B; High molecular weight salivary mucin MG1; MUC-5B; mucin 5, subtype B, tracheobronchial; Mucin-5 subtype B, tracheobronchial; Mucin-5B; Sublingual gland mucin

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Gene Aliases: MG1; MUC-5B; MUC5; MUC5B; MUC9

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UniProt ID: (Human) Q9HC84

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Entrez Gene ID: (Human) 727897

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Function(s)

protein binding

Process(es)

O-glycan processing defense response to bacterium regulation of macrophage activation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MUC5B Polyclonal Antibody

MUC5B Antibody (PA5-82342) in ICC/IF

Product Details

PA5-82342

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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