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Invitrogen
MURF3 Polyclonal Antibody
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FIGURE: 1 / 1
MURF3 Antibody (PA5-88991) in WB
Western blot analysis of MURF3 in extracts of various cell lines. Samples were incubated with MURF3 Polyclonal antibody (Product # PA5-88991) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-88991
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 179-358 of human TRIM54 (NP_9127302)
Conjugate
Form
Liquid
Concentration
2.35 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805278
Product Specific Information
Immunogen sequence: AMLVAGNDRV QAVITQMEEV CQTIEDNSRR QKQLLNQRFE SLCAVLEERK GELLQALARE QEEKLQRVRG LIRQYGDHLE ASSKLVESAI QSMEEPQMAL YLQQAKELIN KVGAMSKVEL AGRPEPGYES MEQFTVRVEH VAEMLRTIDF QPGASGEEEE VAPDGEEGSA GPEEERPDGP; Positive Samples: A-549, Mouse heart; Cellular Location: Cytoplasm, Z line, cytoskeleton, myofibril, sarcomere
Target Information
The protein encoded by this gene contains a RING finger motif and is highly similar to the ring finger proteins RNF28/MURF1 and RNF29/MURF2. In vitro studies demonstrated that this protein, RNF28, and RNF29 form heterodimers, which may be important for the regulation of titin kinase and microtubule-dependent signal pathways in striated muscles. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MuRF; MuRF-3; Muscle-specific RING finger protein; Muscle-specific RING finger protein 3; muscle-specific RING-finger protein; muscle-specific RING-finger protein 3; OTTHUMP00000122598; RING finger protein 30; tripartite motif-containing 54; Tripartite motif-containing protein 54
Gene Aliases: 4930486E09Rik; 4930566I02Rik; MURF; MURF-3; MURF3; RNF30; TRIM54
UniProt ID: (Human) Q9BYV2, (Mouse) Q9ERP3
Entrez Gene ID: (Human) 57159, (Mouse) 58522
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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