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MUTYH Polyclonal Antibody

MUTYH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MUTYH Antibody (19650-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50 (under 40x lens).

MUTYH Antibody in Immunoprecipitation (IP)

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Product Details

19650-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MUTYH Fusion Protein Ag7526 (187-535 aa encoded by BC003178)

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.28 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MPRTAETLQ QLLPGVGRYT AGAIASIAFG QATGVVDGNV ARVLCRVRAI GADPSSTLVS QQLWGLAQQL VDPARPGDFN QAAMELGATV CTPQRPLCSQ CPVESLCRAR QRVEQEQLLA SGSLSGSPDV EECAPNTGQC HLCLPPSEPW DQTLGVVNFP RKASRKPPRE ESSATCVLEQ PGALGAQILL VQRPNSGLLA GLWEFPSVTW EPSEQLQRKA LLQELQRWAG PLPATHLRHL GEVVHTFSHI KLTYQVYGLA LEGQTPVTTV PPGARWLTQE EFHTAAVSTA MKKVFRVYQG QQPGTCMGSK RSQVSSPCSR KKPRMGQQVL DNFFRSHIST DAHSLNSAAQ (187-535 aa encoded by BC003178)

Target Information

MYH (mutY homolog (E. coli )) is a DNA glycosylase mismatch repair enzyme that in conjunction with mutM (OGG1), cleaves adenine residues paired with either oxidized (8-hydroxyguanines) or non-modified guanines in order to correct A/G and A/C mismatches. Repair of most modified and mispaired bases in the genome is initiated by DNA glycosylases, which bind and cleave N-glycosyl bonds to initiate base excision repair. MYH is crucial for the avoidance of mutations resulting from oxidative DNA damage. Multiple N-terminal splice variants of MYH exist in mammalian cells. Increasing levels of MYH in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival. Biallelic MYH germ-line mutations predispose humans to colorectal adenomas and carcinomas. MYH is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MUTYH Actively Contributes to Microglial Activation and Impaired Neurogenesis in the Pathogenesis of Alzheimer's Disease.

Oxidative medicine and cellular longevity

Mizuno Y,Abolhassani N,Mazzei G,Sakumi K,Saito T,Saido TC,Ninomiya T,Iwaki T,Yamasaki R,Kira JI,Nakabeppu Y

Thu Mar 03 00:00:00 UTC 2022

Downregulation of MUTYH contributes to cisplatin‑resistance of esophageal squamous cell carcinoma cells by promoting Twist‑mediated EMT.

Oncology reports

Guo Y,Jia Y,Wang S,Liu N,Gao D,Zhang L,Lin Z,Wang S,Kong F,Peng C,Liu Y

Sun Dec 01 00:00:00 UTC 2019

Potent and specific MTH1 inhibitors targeting gastric cancer.

Cell death & disease

Zhou W,Ma L,Yang J,Qiao H,Li L,Guo Q,Ma J,Zhao L,Wang J,Jiang G,Wan X,Adam Goscinski M,Ding L,Zheng Y,Li W,Liu H,Suo Z,Zhao W

Tue Jun 04 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: A/G-specific adenine DNA glycosylase; Adenine DNA glycosylase; adenine-DNA glycosylase; hMYH; MGC4416; mMYH; MutY homolog; mutY homolog alpha; mutY-like protein; OTTHUMP00000009098; OTTHUMP00000009102; rMYH; RP4-534D1.2; the full-length form type A, 5' region

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Gene Aliases: 5730495A01Rik; MUTYH; Mutyha; Mutyhalpha; Mutyhb; Mutyhbeta; Mutyhc; MYH

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UniProt ID: (Human) Q9UIF7, (Mouse) Q99P21, (Rat) Q8R5G2

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Entrez Gene ID: (Human) 4595, (Mouse) 70603, (Rat) 170841

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Function(s)

DNA binding protein binding DNA N-glycosylase activity MutLalpha complex binding MutLbeta complex binding MutSalpha complex binding MutSbeta complex binding metal ion binding 4 iron, 4 sulfur cluster binding purine-specific mismatch base pair DNA N-glycosylase activity catalytic activity uracil DNA N-glycosylase activity hydrolase activity hydrolase activity, acting on glycosyl bonds single-strand selective uracil DNA N-glycosylase activity DNA-3-methylbase glycosylase activity iron-sulfur cluster binding nicotinamide riboside hydrolase activity nicotinic acid riboside hydrolase activity deoxyribonucleoside 5'-monophosphate N-glycosidase activity

Process(es)

DNA repair base-excision repair cellular response to DNA damage stimulus response to oxidative stress metabolic process mismatch repair depurination

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MUTYH Polyclonal Antibody

MUTYH Antibody (19650-1-AP) in IHC (P)

Product Details

19650-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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