Invitrogen

MYH11 Recombinant Rabbit Monoclonal Antibody (17H3L51)

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MYH11 Antibody (702544) in WB

Western blot analysis was performed on Tissue Extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Lung (Lane 2), Mouse Heart (Lane 3), Rat Ovary (Lane 5) and Whole cell extracts of C2C12 (Lane 4). The blots were probed with Anti-MYH11 Recombinant Rabbit Monoclonal Antibody (Product # 702544, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4,000 dilution). A 227 kDa band corresponding to MYH11 was observed across the... View More

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MYH11 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Expression of MYH11 was observed specifically at high level in Heart Tissue,at medium levels in lung tissue and no expression in Brain tissue using MYH11 Recombinant Rabbit Monoclonal Antibody (product # 702544) in Western blot. {RE}

Product Details

702544

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

Immunohistochemistry (IHC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

17H3L51

Immunogen

Peptides corresponding to human MYH11 [1) aa1915 -aa1933 2) aa1795 - aa1809]

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2734821

Product Specific Information

This antibody is predicted to react with Monkey, Horse, Pig.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

SM-MHC is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmently regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

702544 was used in Immunohistochemistry to establish both direct and indirect roles for ILC2 in peri-aortic CaPO4 injury-induced mouse abdominal aortic aneurysms.

Wed Mar 01 00:00:00 UTC 2023

IgE Contributes to Atherosclerosis and Obesity by Affecting Macrophage Polarization, Macrophage Protein Network, and Foam Cell Formation.

Arteriosclerosis, thrombosis, and vascular biology

Zhang X,Li J,Luo S,Wang M,Huang Q,Deng Z,de Febbo C,Daoui A,Liew PX,Sukhova GK,Metso J,Jauhiainen M,Shi GP,Guo J

702544 was used in Immunohistochemistry to demonstrate that IgE deficiency reduced atherosclerosis lesion burden, lesion lipid deposition, smooth muscle cell and endothelial cell contents, chemokine MCP (monocyte chemoattractant protein)-1 expression and macrophage accumulation.

Sun Mar 01 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: myh11 {ECO:0000312|RGD:3136}; Myosin heavy chain 11; myosin heavy chain 11, smooth muscle; myosin heavy chain 21; Myosin heavy chain, smooth muscle isoform; myosin, heavy chain 11, smooth muscle; myosin, heavy polypeptide 11, smooth muscle; Myosin-11; SMMHC; smooth muscle myosin heavy chain

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Gene Aliases: AAT4; AV071570; FAA4; KIAA0866; MYH11; SM1; SM2; SMHC; SMMHC

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UniProt ID: (Human) P35749

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Entrez Gene ID: (Human) 4629, (Rat) 24582, (Mouse) 17880

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Function(s)

motor activity protein binding calmodulin binding ATP binding structural constituent of muscle actin filament binding actin binding nucleotide binding

Process(es)

smooth muscle contraction metabolic process elastic fiber assembly cardiac muscle fiber development muscle contraction skeletal muscle myosin thick filament assembly mitophagy in response to mitochondrial depolarization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MYH11 Recombinant Rabbit Monoclonal Antibody (17H3L51)

MYH11 Antibody (702544) in WB

Product Details

702544

Western Blot (WB)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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