Invitrogen

MYH6 Monoclonal Antibody (GT565)

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MYH6 Antibody (MA5-27820) in WB

Western blot was performed using Anti-MYH6 Monoclonal Antibody (GT565) (Product # MA5-27820) and a 240 kDa band corresponding to MYH6 was observed only in Mouse Heart when compared to other tissue extracts tested which are reported to be low. Tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Mouse Skeletal Muscle (Lane 2), Mouse Liver (Lane 3), Mouse Brain (Lane 4), Mouse Kidney (Lane 5) and Mouse Lung (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred... View More

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MYH6 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue extracts tested owing to their inherent genetic constitution. Relative expression of MYH6 was observed only in Mouse Heart as compared to Mouse Skeletal Muscle, Mouse Liver, Mouse Brain, Mouse Kidney and Mouse Lung using MYH6 Monoclonal Antibody (GT565) (Product # MA5-27820) in Western Blot. {RE}

Product Details

MA5-27820

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

GT565

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MYH6. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.74 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2735281

Product Specific Information

Positive Control: mouse heart

Predicted Reactivity: Human (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located approximately 4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A remarkable adaptive paradigm of heart performance and protection emerges in response to marked cardiac-specific overexpression of ADCY8.

eLife

Tarasov KV,Chakir K,Riordon DR,Lyashkov AE,Ahmet I,Perino MG,Silvester AJ,Zhang J,Wang M,Lukyanenko YO,Qu JH,Barrera MC,Juhaszova M,Tarasova YS,Ziman B,Telljohann R,Kumar V,Ranek M,Lammons J,Bychkov R,de Cabo R,Jun S,Keceli G,Gupta A,Yang D,Aon MA,Adamo L,Morrell CH,Otu W,Carroll C,Chambers S,Paolocci N,Huynh T,Pacak K,Weiss R,Field L,Sollott SJ,Lakatta EG

MA5-27820 was used in Western Blotting to show that TGAC8 mice show classical cardiac hypertrophy markers were absent in TGAC8, and that total left ventricular (LV) mass was not increased: a reduced LV cavity volume in TGAC8 was encased by thicker LV walls harboring an increased number of small cardiac myocytes, and a network of small interstitial proliferative non-cardiac myocytes compared to wild type (WT) littermates compared to wild type littermates.

Wed Dec 14 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: alpha cardiac MHC; alpha myosin; cardiac myosin heavy chain alpha isoform; cardiomyopathy, hypertrophic 1; MyHC-alpha; myosin 6; Myosin heavy chain 6; Myosin heavy chain, cardiac muscle alpha isoform; Myosin heavy chain, cardiac muscle alpha isoform (MyHC-alpha); myosin heavy chain, cardiac muscle, adult; myosin, heavy polypeptide 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1); Myosin-6

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Gene Aliases: A830009F23Rik; AA517445; alpha-MHC; ASD3; CMD1EE; CMH14; MYH6; MYHC; Myhc-a; MYHCA; SSS3

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UniProt ID: (Human) P13533, (Mouse) Q02566

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Entrez Gene ID: (Human) 4624, (Mouse) 17888

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Function(s)

microfilament motor activity actin binding calmodulin binding ATP binding ATPase activity myosin phosphatase activity protein kinase binding actin-dependent ATPase activity nucleotide binding motor activity protein binding calcium-dependent ATPase activity identical protein binding protein homodimerization activity protein heterodimerization activity

Process(es)

in utero embryonic development regulation of the force of heart contraction regulation of heart rate muscle contraction striated muscle contraction adult heart development visceral muscle development regulation of heart contraction metabolic process regulation of blood pressure actin filament-based movement muscle filament sliding myofibril assembly BMP signaling pathway regulation of ATPase activity sarcomere organization ATP metabolic process cardiac muscle fiber development atrial cardiac muscle tissue morphogenesis ventricular cardiac muscle tissue morphogenesis cardiac muscle contraction canonical Wnt signaling pathway regulation of heart growth protein dephosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MYH6 Monoclonal Antibody (GT565)

MYH6 Antibody (MA5-27820) in WB

Product Details

MA5-27820

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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