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Invitrogen
MYH9 Monoclonal Antibody (K1E005_16A9)
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MYH9 Antibody (MA5-55857) in IHC (P)
Immunohistochemistry analysis of MYH9 in paraffin-embedded kidney tissue. Sample was incubated with MYH9 monoclonal antibody (Product # MA5-55857) at a dilution of 2.5 µg/mL (RT, 1 hour). Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. Po... View More
Product Details
MA5-55857
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
K1E005_16A9
Immunogen
Recombinant human MYH9 (immunogen range is 1621-1960).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_3668089
Product Specific Information
Sequence of this protein is as follows: DLEAHIDSAN KNRDEAIKQL RKLQAQMKDC MRELDDTRAS REEILAQAKE NEKKLKSMEA EMIQLQEELA AAERAKRQAQ QERDELADEI ANSSGKGALA LEEKRRLEAR IAQLEEELEE EQGNTELIND RLKKANLQID QINTDLNLER SHAQKNENAR QQLERQNKEL KVKLQEMEGT VKSKYKASIT ALEAKIAQLE EQLDNETKER QAACKQVRRT EKKLKDVLLQ VDDERRNAEQ YKDQADKAST RLKQLKRQLE EAEEEAQRAN ASRRKLQREL EDATETADAM NREVSSLKNK LRRGDLPFVV PRRMARKGAG DGSDEEVDGK ADGAEAKPAE
Target Information
TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the -gamma-secretase but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the -gamma- and epsilon-sites and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer's disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased -gamma-secretase activity but not epsilon-secretase activity, and increased Abeta production, demonstrating that TMP21 can modulate -gamma-secretase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cellular myosin heavy chain, type A; MGC104539; Myosin heavy chain 9; Myosin heavy chain, non-muscle IIa; Myosin-9; myosin9; NMHC-II A; NMMHC II-a; NMMHC IIA; NMMHC-A; non-muscle myosin heavy chain 9; Non-muscle myosin heavy chain A; Non-muscle myosin heavy chain IIa; non-muscle myosin heavy polypeptide 9; nonmuscle myosin heavy chain II-A
Gene Aliases: BDPLT6; DFNA17; EPSTS; FTNS; MHA; MYH9; NMHC-II-A; NMMHC-IIA; NMMHCA
UniProt ID: (Human) P35579
Entrez Gene ID: (Human) 4627
microfilament motor activity
motor activity
actin binding
protein binding
calmodulin binding
ATP binding
ATPase activity
protein domain specific binding
actin-dependent ATPase activity
protein homodimerization activity
protein anchor
ADP binding
poly(A) RNA binding
actin filament binding
cadherin binding involved in cell-cell adhesion
meiotic spindle organization
cytokinesis
angiogenesis
in utero embryonic development
establishment of T cell polarity
membrane protein ectodomain proteolysis
phagocytosis, engulfment
integrin-mediated signaling pathway
myoblast fusion
regulation of cell shape
protein transport
actin filament-based movement
platelet formation
monocyte differentiation
actomyosin structure organization
actin cytoskeleton reorganization
uropod organization
blood vessel endothelial cell migration
leukocyte migration
establishment of meiotic spindle localization
platelet aggregation
negative regulation of actin filament severing
positive regulation of protein processing in phagocytic vesicle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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