Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
MYL12A Antibody (PA5-97190) in WB
Western blot analysis of MYL12A in extracts of various cell lines. Samples were incubated with MYL12A Polyclonal antibody (Product # PA5-97190) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-97190
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 92-171 of human MYL12A (NP_0064621)
Conjugate
Form
Liquid
Concentration
2.87 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808992
Product Specific Information
Immunogen sequence: KLNGTDPEDV IRNAFACFDE EATGTIQEDY LRELLTTMGD RFTDEEVDEL YREAPIDKKG NFNYIEFTRI LKHGAKDKDD; Positive Samples: LO2, A375, HeLa, K-562, Jurkat
Target Information
This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Epididymis secretory protein Li 24; HEL-S-24; MLC-2; MLC-2A; MLC-2B; MLC20; Myosin regulatory light chain 12A; Myosin regulatory light chain 12B; Myosin regulatory light chain 2, nonsarcomeric; Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; myosin regulatory light chain 3; Myosin regulatory light chain MRLC2; Myosin regulatory light chain MRLC3; Myosin RLC; myosin, light chain 12A, regulatory, non-sarcomeric; myosin, light polypeptide, regulatory, non-sarcomeric (20kD); SHUJUN-1
Gene Aliases: HEL-S-24; MLC-2B; MLCB; MRCL3; MRLC2; MRLC3; MYL12A; MYL12B; MYL2B; MYLC2B; RLC
UniProt ID: (Human) P19105
Entrez Gene ID: (Human) 10627
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support