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Invitrogen
MYLIP Polyclonal Antibody
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MYLIP Antibody (PA5-96524) in WB
Western blot analysis of MYLIP in extracts of various cell lines. Samples were incubated with MYLIP Polyclonal antibody (Product # PA5-96524) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-96524
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 266-445 of human MYLIP (NP_0373942)
Conjugate
Form
Liquid
Concentration
1.03 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808326
Product Specific Information
Immunogen sequence: RAITETHAFY RCDTVTSAVM MQYSRDLKGH LASLFLNENI NLGKKYVFDI KRTSKEVYDH ARRALYNAGV VDLVSRNNQS PSHSPLKSSE SSMNCSSCEG LSCQQTRVLQ EKLRKLKEAM LCMVCCEEEI NSTFCPCGHT VCCESCAAQL QSCPVCRSRV EHVQHVYLPT HTSLLNLTVI; Positive Samples: Jurkat, Mouse kidney, Mouse liver, Mouse spleen, Rat brain; Cellular Location: Cell membrane, Cytoplasm, Peripheral membrane protein
Target Information
The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: band 4.1 superfamily member BZF1; cellular modulator of immune recognition (c-MIR); E3 ubiquitin ligase-inducible degrader of the low density lipoprotein receptor; E3 ubiquitin-protein ligase MYLIP; Idol; Inducible degrader of the LDL-receptor; MIR; Myosin regulatory light chain interacting protein; Myosin regulatory light chain-interacting protein; RING-type E3 ubiquitin transferase MYLIP
Gene Aliases: 9430057C20Rik; BM-023; BZF1; IDOL; MIR; MYLIP; PP5242
UniProt ID: (Human) Q8WY64, (Mouse) Q8BM54, (Rat) D3ZDI6
Entrez Gene ID: (Human) 29116, (Mouse) 218203, (Rat) 306825
ubiquitin-protein transferase activity
protein binding
cytoskeletal protein binding
zinc ion binding
ligase activity
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
movement of cell or subcellular component
nervous system development
negative regulation of low-density lipoprotein particle clearance
protein ubiquitination
protein destabilization
regulation of low-density lipoprotein particle receptor catabolic process
cholesterol homeostasis
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
positive regulation of protein catabolic process
response to low-density lipoprotein particle
protein polyubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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