Proteintech

MYLK Polyclonal Antibody

MYLK Antibody in Immunocytochemistry (ICC/IF)

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MYLK Antibody (21642-1-AP) in ICC/IF

Immunofluorescent analysis of Hela cells, using MYLK antibody 21642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

MYLK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

21642-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:400

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MYLK Fusion Protein Ag16306 (1748-1914 aa encoded by BC113456)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Smooth muscle MYLK generates three different proteins: long MYLK, short MYLK, and telokin. The 130 kDa smooth muscle, or short, MYLK (130 kDa) is expressed in smooth muscles. It has 8 isoforms produced by alternative splicing and alternative initiation and this MYLK antibody can recognize these isoforms.

Immunogen sequence: TGNAVRAIG RLSSMAMISG LSGRKSSTGS PTSPLNAEKL ESEEDVSQAF LEAVAEEKPH VKPYFSKTIR DLEVVEGSAA RFDCKIEGYP DPEVVWFKDD QSIRESRHFQ IDYDEDGNCS LIISDVCGDD DAKYTCKAVN SLGEATCTAE LIVETMEEGE GEGEEEEE (1748-1914 aa encoded by BC113456)

Target Information

MYLK (MLCK) is a serine/threonine kinase that phosphorylates myosin regulatory light chains to assist in the production of contractile activity (myosin-actin interaction). MLCK is responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Heat-Killed Lacticaseibacillus paracasei Repairs Lipopolysaccharide-Induced Intestinal Epithelial Barrier Damage via MLCK/MLC Pathway Activation.

Nutrients

Xie Z,Zhang G,Liu R,Wang Y,Tsapieva AN,Zhang L,Han J

Tue Apr 04 00:00:00 UTC 2023

The m⁶A methyltransferase METTL3 promotes trophoblast cell invasion by regulating MYLK expression.

Placenta

Zhao J,Ding H,Ding J,Shi X,He Y,Zhu H,Yuan H,Zhang T,Zhang J

Tue Nov 01 00:00:00 UTC 2022

Intestinal Flora-Derived Kynurenic Acid Protects Against Intestinal Damage Caused by Candida albicans Infection via Activation of Aryl Hydrocarbon Receptor.

Frontiers in microbiology

Wang Z,Yin L,Qi Y,Zhang J,Zhu H,Tang J

Fri Aug 05 00:00:00 UTC 2022

Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.

Frontiers in medicine

He F,Ding H,Zhou Y,Wang Y,Xie J,Yang S,Zhu Y

Sun May 01 00:00:00 UTC 2022

Protective effects of curcumin against rat intestinal inflammation‑related motility disorders.

Molecular medicine reports

Yao Y,Luo R,Xiong S,Zhang C,Zhang Y

Sat May 01 00:00:00 UTC 2021

Lanthanum chloride causes blood-brain barrier disruption through intracellular calcium-mediated RhoA/Rho kinase signaling and myosin light chain kinase.

Metallomics : integrated biometal science

Wu J,Yang J,Yu M,Sun W,Han Y,Lu X,Jin C,Wu S,Cai Y

Wed Dec 23 00:00:00 UTC 2020

Doxorubicin induces detrusor smooth muscle impairments through myosin dysregulation, leading to a risk of lower urinary tract dysfunction.

American journal of physiology. Renal physiology

Iguchi N,Dönmez Mİ,Carrasco A,Wilcox DT,Pineda RH,Malykhina AP,Cost NG

Mon Jul 01 00:00:00 UTC 2019

Aryl Hydrocarbon Receptor Activation Modulates Intestinal Epithelial Barrier Function by Maintaining Tight Junction Integrity.

International journal of biological sciences

Yu M,Wang Q,Ma Y,Li L,Yu K,Zhang Z,Chen G,Li X,Xiao W,Xu P,Yang H

Tue Feb 12 00:00:00 UTC 2019

Aryl Hydrocarbon Receptor Activation Modulates Intestinal Epithelial Barrier Function by Maintaining Tight Junction Integrity.

International journal of biological sciences

Yu M,Wang Q,Ma Y,Li L,Yu K,Zhang Z,Chen G,Li X,Xiao W,Xu P,Yang H

Tue Feb 12 00:00:00 UTC 2019

Aryl Hydrocarbon Receptor Activation Modulates Intestinal Epithelial Barrier Function by Maintaining Tight Junction Integrity.

International journal of biological sciences

Yu M,Wang Q,Ma Y,Li L,Yu K,Zhang Z,Chen G,Li X,Xiao W,Xu P,Yang H

Tue Feb 12 00:00:00 UTC 2019

Aryl Hydrocarbon Receptor Activation Modulates Intestinal Epithelial Barrier Function by Maintaining Tight Junction Integrity.

International journal of biological sciences

Yu M,Wang Q,Ma Y,Li L,Yu K,Zhang Z,Chen G,Li X,Xiao W,Xu P,Yang H

Tue Feb 12 00:00:00 UTC 2019

Dihydroartemisinin counteracts fibrotic portal hypertension via farnesoid X receptor-dependent inhibition of hepatic stellate cell contraction.

The FEBS journal

Xu W,Lu C,Zhang F,Shao J,Yao S,Zheng S

Sun Jan 01 00:00:00 UTC 2017

Transmigrated neutrophils in the intestinal lumen engage ICAM-1 to regulate the epithelial barrier and neutrophil recruitment.

Mucosal immunology

Sumagin R,Robin AZ,Nusrat A,Parkos CA

Tue Jul 01 00:00:00 UTC 2014

Transmigrated neutrophils in the intestinal lumen engage ICAM-1 to regulate the epithelial barrier and neutrophil recruitment.

Mucosal immunology

Sumagin R,Robin AZ,Nusrat A,Parkos CA

Tue Jul 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: Kinase-related protein; KRP; MLCK; mylk; Myosin light chain kinase, smooth muscle; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase; Telokin

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Gene Aliases: 9530072E15Rik; A930019C19Rik; AAT7; AW489456; KRP; MLCK; MLCK1; MLCK108; MLCK210; MSTP083; MYLK; MYLK1; nmMlck; smMLCK

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UniProt ID: (Human) Q15746

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Entrez Gene ID: (Human) 4638, (Mouse) 107589, (Rat) 288057

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Function(s)

actin binding myosin light chain kinase activity protein binding calmodulin binding ATP binding metal ion binding nucleotide binding protein kinase activity protein serine/threonine kinase activity calmodulin-dependent protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups enzyme binding

Process(es)

positive regulation of defense response to virus by host protein phosphorylation smooth muscle contraction actin filament organization tonic smooth muscle contraction phosphorylation positive regulation of cell migration bleb assembly positive regulation of calcium ion transport aorta smooth muscle tissue morphogenesis cellular hypotonic response positive regulation of wound healing mitophagy in response to mitochondrial depolarization muscle contraction xenophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MYLK Polyclonal Antibody

MYLK Antibody (21642-1-AP) in ICC/IF

Product Details

21642-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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