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Invitrogen
MYLK Recombinant Rabbit Monoclonal Antibody (SU40-06)
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Product Details
MA5-32136
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SU40-06
Immunogen
Synthetic peptide within Human MYLK aa 1,865-1,914
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2809426
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
MYLK (MLCK) is a serine/threonine kinase that phosphorylates myosin regulatory light chains to assist in the production of contractile activity (myosin-actin interaction). MLCK is responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Kinase-related protein; KRP; MLCK; mylk; Myosin light chain kinase, smooth muscle; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase; Telokin
Gene Aliases: 9530072E15Rik; A930019C19Rik; AAT7; AW489456; KRP; MLCK; MLCK1; MLCK108; MLCK210; MSTP083; MYLK; MYLK1; nmMlck; smMLCK
UniProt ID: (Human) Q15746
Entrez Gene ID: (Human) 4638, (Mouse) 107589, (Rat) 288057
actin binding
myosin light chain kinase activity
protein binding
calmodulin binding
ATP binding
metal ion binding
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
calmodulin-dependent protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
enzyme binding
positive regulation of defense response to virus by host
protein phosphorylation
smooth muscle contraction
actin filament organization
tonic smooth muscle contraction
phosphorylation
positive regulation of cell migration
bleb assembly
positive regulation of calcium ion transport
aorta smooth muscle tissue morphogenesis
cellular hypotonic response
positive regulation of wound healing
mitophagy in response to mitochondrial depolarization
muscle contraction
xenophagy
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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