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Invitrogen
MYLK2 Polyclonal Antibody
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FIGURE: 1 / 2
MYLK2 Antibody (PA5-115609) in ICC/IF
Immunocytochemistry analysis of MYLK2 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MYLK2 polyclonal antibody (Product # PA5-115609) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-115609
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MYLK2(Accession Q9H1R3), corresponding to amino acid residues P264-K314.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2900244
Product Specific Information
Antibody detects endogenous levels of total MYLK2.
Target Information
MYLK2 (skMLCK) is a calcium/calmodulin-dependent serine/threonine kinase expressed exclusively in adult skeletal muscle. MYLK2 encodes a myosin light chain kinase, a calcium/calmodulin dependent enzyme, that is exclusively expressed in adult skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MLCK2; myosin light chain kinase 2, skeletal muscle; Myosin light chain kinase 2, skeletal/cardiac muscle; myosin, light polypeptide kinase 2
Gene Aliases: 9830004H17Rik; BB138278; Klmc; KMLC; MLCK; MLCK2; MYLK2; skMLCK
UniProt ID: (Human) Q9H1R3, (Mouse) Q8VCR8, (Rat) P20689
Entrez Gene ID: (Human) 85366, (Mouse) 228785, (Rat) 117558
calmodulin-dependent protein kinase activity
myosin light chain kinase activity
protein binding
calmodulin binding
ATP binding
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
myosin light chain binding
striated muscle contraction
neuromuscular synaptic transmission
positive regulation of gene expression
skeletal muscle satellite cell differentiation
peptidyl-threonine phosphorylation
regulation of muscle filament sliding
skeletal muscle cell differentiation
protein autophosphorylation
cardiac muscle tissue morphogenesis
cardiac muscle contraction
protein phosphorylation
water transport
regulation of muscle contraction
phosphorylation
positive regulation of fast-twitch skeletal muscle fiber contraction
regulation of MAPK cascade
regulation of neuronal synaptic plasticity
synaptic vesicle transport
regulation of cell cycle
cardiac muscle cell differentiation
muscle contraction
peptidyl-serine phosphorylation
positive regulation of cellular component movement
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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